Bottleneck　issues　such　as　difficult　biofilm　culturing,　time-consuming　granular　formation　and　excessive　nitrate　production,　always　limited　the　application　of　anammox　processes.　By　using　the　common　but　meaningful　suspended　activated　sludge　instead　of　biofilm　or　granule　activated　sludge,　a　combined　anammox　process,　simultaneous　partial　nitrification,　anammox　and　denitrification　(SNAD),　was　established　in　a　sequencing　batch　reactor　(SBR)　in　this　study　to　treat　real　domestic　sewage　and　was　operated　for　300　days.　Results　indicated　that　a　high　ammonia　removal　rate　and　nitrogen　removal　rate　(1.1　and　1.03　(kgN/(m(3)center　dot　d)),　respectively)　were　achieved　during　the　baseline　phase　treating　high　ammonia　wastewater　(350-550　mg/L).　During　the　experimental　phase　treating　domestic　sewage　with　a　C/N　of　3-3.5,　86.1%　of　total　nitrogen　removal　efficiency　was　achieved　and　only　1.02　mg/L　of　nitrate　concentration　was　detected　in　the　effluent　with　an　averagely　64.6　mg/L　of　ammonium　in　the　influent.　Nitrogen　mass　balance　analysis　demonstrated　that　about　89%　of　total　nitrogen　(TN)　was　removed　by　anammox　and　the　remaining　11%　of　TN　was　removed　by　denitrification.　Bacterial　abundance　of　Candidatus　Brocadia　and　Thauera　increased　from　0.02%　to　0.5%　and　0.39%　to　2.24%　(from　baseline　phase　to　experimental　phase),　respectively,　which　made　an　essential　contribution　to　the　nitrogen　removal.　The　advanced　nitrogen　removal　performance　reached　in　the　SNAD　process　showed　the　possibility　of　using　suspended　sludge　as　a　substitute　of　biofilm　and　granule　sludge　concerning　anammox,　and　the　process　could　treat　real　domestic　sewage　with　a　C/N　of　3-3.5　without　pretreatment　of　chemical　oxygen　demand　(COD).