The　two-stage　aeration　operational　mode　was　used　in　an　aerobic　granular　sludge　(AGS)　sequencing　batch　reactor,　under　different　high　and　low　aeration　time　distribution.　Obtained　results　showed　that　the　introduction　of　the　low　aeration　stage　provided　a　good　denitrification　environment,　alleviating　the　competition　for　nutrients　between　heterotrophic　aerobic　microorganisms.　This　method　had　quickly　realized　the　process　of　short　range　simultaneous　nitrification　and　denitrification　(SSND),　the　total　nitrogen　removal　rate　increased　from　40%　to　about　75%.　The　ratio　of　PN/PS　rose　from　1.27　to　the　highest　value　of　1.81,　which　improved　the　mass　transfer　efficiency　in　a　single　particle　and　enhanced　the　stability　of　AGS.　Furthermore,　it　was　known　from　the　results　of　confocal　laser　scanning　microscope　(CLSM)　and　high-throughput　pyrosequencing　and　phylogenetic　assignment　that　the　nitrite-oxidizing　bacteria　(NOB)　were　not　completely　washed,　but　rather　stored　in　dead　cells,　in　the　inner　layer　of　AGS.　Consequently,　this　method　could　save　the　energy　needed　for　aeration　and　improve　the　nitrogen　removal　performance　and　stability　of　AGS.