Objective:　To　study　the　effect　of　anti-HIV-1　virus　with　extracts　from　the　tuber　of　Typhonium　giganteum,　so　as　to　discover　new　and　high　efficient　anti-HIV-1　leading　compounds　from　natural　products　and　Chinese　materia　medica.　Methods:　Extract　from　the　tuber　of　T.　giganteum　was　isolated　and　purified　by　the　way　of　phytochemistry,　such　as　column　chromatography.　Then　anti-HIV-1　activities　of　the　extracts　were　assessed　by　in　vitro　method,　MT4　cells　and　HIV　III　B　virus　were　used　for　the　experiment.　The　results　were　judged　by　cytopathic　effect　(CPE)　method　and　p24　antigen　assay　method.　Mechanism　studies　were　carried　out　by　BIA　techniques　(BIACORE®　3000　molecule　coupled　equipment).　Results:　The　extracts　of　T.　giganteum　showed　potential　anti-HIV-1　activities.　Two　of　them　showed　gp-41　transmembrance　protein　and　Vif　protein　expression　inhibition,　respectively.　Conclusion:　These　extracts　in　the　tuber　of　T.　giganteum　might　rewardingly　contribute　to　anti-HIV-1　activities,　which　could　be　developed　to　be　more　efficient　and　less　toxic　leading　compounds　in　the　further.