This　paper　presents　a　novel　manipulation　method　for　micro-objects　using　acoustically　oscillating　bubbles　with　a　controllable　position　based　on　the　gas　permeability　of　polydimethylsiloxane.　The　oscillating　bubble　trapped　within　the　side　channel　attracts　the　neighboring　micro-objects,　and　the　position　of　the　air-liquid　interface　is　controlled　by　generating　temporary　pressure　difference　between　the　side　channel　and　the　air　channel.　To　demonstrate　the　feasibility　of　the　method　in　technological　applications,　polystyrene　microparticles　of　10　mu　m　in　diameter　were　successfully　captured,　transported,　and　released.　The　influence　of　pressure　difference　on　the　movement　speed　of　the　air-liquid　interface　was　demonstrated　in　our　experiments,　and　the　manipulation　performance　was　also　characterized　by　varying　the　frequency　of　the　acoustic　excitation　and　the　pressure　difference.　Since　the　bubble　generation　and　the　air-liquid　interface　movement　in　our　manipulation　method　do　not　need　any　electrochemical　reaction　and　any　high　temperature,　this　on-chip　manipulation　method　provides　a　controllable,　efficient,　and　noninvasive　tool　for　handling　microobjects　such　as　particles,　cells,　and　other　entities.　The　whole　manipulation　process,　including　capturing,　transporting,　and　releasing　of　particles,　spent　less　than　1　min.　It　can　be　used　to　select　the　cells　and　particles　in　the　microfluidic　device　or　change　the　cell　culture　medium.　Published　by　AIP　Publishing.