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Treatment of real leachate from municipal landfill with high ammonia nitrogen content was investigated by using lab-scale anoxic/anaerobic UASB-A/O process. On the basis of achieving simultaneous COD and nitrogen removal, how to achieve and stabilize partial nitrification in the A/O reactor was studied. Denitrification and methanogenesis were conducted in UASB reactor, and the average removal rate of organics and NOx--N were 5.3, 1.1 kg/(m3·d), respectively. Partial nitrification was achieved (nitrite accumulation ratio was above 50%) after 54 days operation, and after 70days, nitrite accumulation ratio in A/O reactor reached above 90% at ambient temperature of 12 to 30.6°C. Stable and long term partial nitrification was achieved due to the cooperative effect of free acid (FA) and free nitrous acid (FAN). Destruction of partial nitrification caused by extended aeration was avoided by using pH as process control parameter. Nitrifying population was optimized by establishing favorable environment of AOB and inhibiting the growth of NOB, then NOB was washed out from the biological system, which was proved by fluorescence in situ hybridization (FISH).
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