The　separation　and　analytical　technique　is　one　of　the　core　technologies　in　proteomic　studies.　The　advances　in　the　applications　of　separation　and　analytical　techniques　in　proteomic　studies　on　protein　expression　profiling;　protein　modification　profiling;　protein　complex　and　interaction　network　as　well　as　proteome　quantitation　are　reviewed;　and　89　references　are　cited.