Increasingly　serious　microbial　infections　call　for　the　development　of　new　simpler　methods　for　the　precise　diagnosis　and　specific　inhibition　of　such　pathogens.　In　this　work,　a　peptide　mineralized　Au　cluster　probe　was　applied　as　a　new　simplified　strategy　to　both　recognize　and　inhibit　a　single　bacteria　species　of　Staphylococcus　aureus　(S.　aureus)　simultaneously.　The　probes　are　composed　of　peptides　and　Au　clusters.　Moreover,　the　peptides　specifically　target　S.　aureus　cells　and　the　Au　clusters　provide　fluorescent　imaging　and　have　an　antibacterial　effect.　These　new　probes　enable　the　simultaneous　specific　detection　and　effective　destruction　S.　aureus　cells　in　situ.