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Factors of rapid start-up for partial nitrification were investigated in the sequencing batch reactor (SBR) treating real domestic wastewater with low CODATN ratio. It was concluded that the optimal conditions consisted of temperature 30°C, dissolved oxygen (DO) 2.0mg/L and sludge retention time(SRT) 7 days. Partial nitrification to nitrite was achieved under real-time control in 32 cycles. The nitrite accumulation rate (NO2--N/NOx--N) was over 90% when the TN elimination efficiency was above 95%. In the subsequent 64 days, the system was also successfully operated at room temperature (20~24°C). Fluorescent in-situ hybridization (FISH) analysis showed that the population of ammonia oxidizing bacteria (AOB) increased by 38.9% while nitrite oxidizing bacteria(NOB) decreased by 53.2% after 32 cycles. NOB could be gradually washed out by the online control of DO and aerobic duration, so steady partial nitrification and nitrogen removal efficiency could be achieved and enhanced, respectively.
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