Silver　nanomaterials　have　remarkable　application　in　biomedical　detection　due　to　their　unique　surface　plasmon　resonance　(SPR)　characteristics.　It　can　be　used　for　surface-enhanced　Raman　scattering　(SERS)　and　surface-enhanced　fluorescence　(SEF).　Current　research　elaborates　a　technique　for　improvement　of　SYBR　Green　I　detection　obtained　from　surface-enhanced　Raman　scattering　(SERS)　and　surface-enhanced　fluorescence　(SEF)　by　silver　nanoparticles　with　the　average　size　about　70　nm.　Primarily,　SYBR　Green　I　is　an　important　fluorescent　dye　used　in　polymerase　chain　reaction　(PCR).　It　is　found　that　both　Raman　and　fluorescence　can　be　used　for　detection　of　this　dye.　Furthermore,　the　enhanced　efficiency　of　the　Raman　and　fluorescence　by　SERS　and　SEF　is　observed　in　this　study,　the　enhancement　factor　for　Raman　signals　is　3.2　x　10(3),　and　the　fluorescence　intensity　bincreased　two　times　by　SEF.　The　quantitative　detection　of　SYBR　Green　I　by　SERS　and　SEF　can　be　achieved.　The　present　work　can　be　used　to　improve　the　detection　of　SYBR　Green　I　by　SERS　and　SEF.　It　would　also　be　employed　for　high-sensitive　detection　of　other　materials　in　the　future.