Tumor　cell　invasion　is　pivotal　to　the　development,　metastasis,　and　prognosis　of　tumors.　It　is　reported　that　the　invasive　ability　of　tumor　cells　is　mainly　dependent　on　the　expression　levels　of　membrane　type-1　matrix　metalloproteinase　(MT1-MMP)　and　integrin　alpha(V)beta(3)　proteins　on　cell　membranes.　To　precisely　distinguish　between　tumor　cells　with　different　invasive　abilities,　it　is　important　to　establish　a　highly　sensitive　and　precise　quantification　method　to　differentiate　the　expression　levels　of　MT1-MMP　and　integrin　alpha(V)beta(3)　in　the　same　single　tumor　cell　at　the　same　time.　Herein,　two　functional　peptides　to　construct　red-emissive　Au-26　clusters　and　green-emissive　Ag-12　clusters　are　reported.　Moreover,　the　Au-26　clusters　and　Ag-12　clusters　have　the　ability　to　specifically　target　MT1-MMP　and　integrin　alpha(V)beta(3),　respectively,　in　the　same　single　cell　at　the　same　time.　By　utilizing　the　fluorescent　properties　and　metallic　compositions　of　metal　clusters,　the　MT1-MMP　and　integrin　alpha(V)beta(3)　levels　of　the　more　invasive　SiHa　cells　or　the　less　invasive　HeLa　cells　are　simultaneously　and　quantitatively　differentiated　via　laser　ablation　inductively　coupled　plasma　mass　spectrometry.　This　method　of　quantitatively　detecting　multiple　invasive　proteins　on　the　same　cell　is　of　great　value　for　accurately　diagnosing　aggressive　tumors　and　monitoring　the　invasiveness　of　these　tumors.