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A sludge population optimization strategy aims to select the ammonia oxidation bacteria (AOB) by using sodium chloride (NaCl) as a selective inhibitor to nitrite oxidation bacteria (NOB) is applied in biological nitrogen removal process during municipal wastewater treatment using a sequencing batch reactor(SBR). Different salinity tests contributed to the best inhibition salinity chosen were applied. In order to optimize nitrifying microbial communities to get steady nitrite pathway, 7.6 g/L salinity and 4 month salt inhibition period were applied. FISH analysis indicated that AOB (Nitrosospira) became the dominant nitrifying bacteria and NOB (Nitrobacter) had been washed out of the activated sludge. An understanding of salt inhibition mechanism on NOB is also discussed. © 2010 Peking University.
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