Graphene　oxide　has　great　potentials　for　preparing　multilayers　with　polyelectrolytes　due　to　its　abundant　carboxyl　groups.　The　as-resulting　polyelectrolyte　multilayers　(PEMs)　showed　excellent　performance　and　significant　improved　stability　in　membrane　separation　applications.　However,　the　stability　enhancement　of　GO　nanosheets　in　the　GO　incorporated　PEMs　is　still　lack　of　systematic　discussion.　In　this　study,　the　enhancements　of　GO　were　confirmed　by　comparing　the　pristine　and　GO　incorporated　PEMs　membranes　through　layer-by-layer　self-assembly　strategy.　The　membranes　were　post-treated　with　NaCIO/NaOH/HCI　solutions,　and　the　morphologies,　chemical　properties,　and　separation　capabilities　of　the　multilayer　membranes　before　and　after　treatment　were　compared.　The　results　indicate　that　the　probable　mechanism　for　the　enhancements　is　attributed　to　the　confinement　effects　of　GO　nanosheets　on　the　migration　and　rearrangement　of　polyelectrolyte　chains.　Thus,　we　anticipate　that　this　study　could　provide　a　comprehensive　understanding　of　GO　based　multilayers　and　an　approach　to　prepare　stable　PEMs　membranes　with　GO　nanosheets　and　other　2D　materials.