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A quick process was proposed to screen PAOs based on the aerobic/anaerobic cultivation. 15 strains, which had over 60% phosphate removal rate, were screened out and then tested by 16S rDNA and biochemical analyses. Except 2 strains of Bacillus sp., the other strains were γ-Proteobacteria, mainly Klebsiella sp. and Pseudomonas sp.. The phosphate removal experiment indicated that the pH values at the end of fermentation increased with the increase in the removal capacity of the PAOs. The phosphate removal rate increased from 52% to 73.3% after adding the PAOs strain screened into active sludge. The addition of the PAOs strain was an effective way to enhance the phosphate removal capacity of activated sludge.
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