This　study　compares　the　microstructure　and　tensile　properties　of　Ti6Al4V　components　fabricated　by　laser　direct　metal　deposition　(LDMD)　additive　manufacturing　(AM)　in　the　transverse　and　longitudinal　directions.　The　results　show　anisotropic　tensile　properties　with　the　transverse　direction　having　high　tensile　and　fracture　strengths　and　the　longitudinal　direction　having　a　high　elongation　and　reduction　of　cross　section.　The　anisotropic　mechanical　properties　are　attributed　to　the　anisotropic　microstructural　distribution.　The　transverse　tensile　specimen　is　composed　of　short　columnar　prior-beta　grains　which　grow　perpendicular　to　the　tensile　direction,　and　have　a　lamellar　structure.　Along　the　beta　grain　boundary,　alpha(GB)　and　large　alpha　colonies　were　identified.　However,　the　longitudinal　specimen　shows　that　the　long　beta　structure　is　parallel　to　the　tensile　axis　and　that　the　microstructure　is　composed　of　basket-woven　alpha　phases　with　shorter　alpha　plates　and　smaller　colony　sizes　compared　with　those　in　the　transverse　specimen.　The　fracture　mechanism　induced　by　the　anisotropic　microstructure　along　the　transverse　and　longitudinal　directions　was　compared　by　examining　the　fracture　process　in　real-time　using　uniaxial　in-situ　scanning　electron　microscopy　(SEM)　tensile　testing.　The　results　show　that　shear　fracture,　which　is　caused　by　the　vertical　beta　grain　boundaries　and　large　alpha　colonies　with　long　alpha　plates,　occurs　in　the　transverse　specimen.　The　shear　mode　is　the　main　reason　behind　the　enhanced　tensile　strength　and　fracture　strength　due　to　the　high　resistance　to　microcrack　propagation.　However,　in　the　longitudinal　specimens,　symmetric　necking　behavior　due　to　the　fine　a　grains　resulted　in　uniform　deformation　of　the　grains　on　both　sides　of　the　grain　boundaries,　inducing　greater　elongation.