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In order to investigate the influences of activated sludge aerobic granulation process on microbial diversity and the differences of microbial community structure in aerobic granular sludge (AGS) which was cultivated by different carbon sources or different granulation methods, the microbial community structure of seed sludge and AGS (AGS with artificial wastewater, AGS with real domestic wastewater by adding diatomite and powered activated carbon respectively) were analyzed by PCR-DGGE. The activated sludge aerobic granulation process led to a low microbial diversity. The bacteria which might impact the stability of granular sludge were eliminated. However, the bacteria which were capable of phosphorus accumulation, denitrification or bio-degradation of refractory organic substances were retained. In the activated sludge aerobic granulation process the ammonia oxidation bacteria (AOB) were enriched to a certain extent. Compared with the seed activated sludge, the diversity index and evenness index of AOB were increased. The dominant bacterial communities in AGS were Proteobacteria, Bacteroidetes and uncultured bacterium. The dominant AOB in AGS were all belonged to Nitrosomonas. © 2016, Chinese Society for Environmental Sciences. All right reserved.
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