Based　on　simultaneous　nitrification　and　denitrification　(SND)　in　a　continuous　anoxic/aerobic　flow-separation　biofilm　system,　the　influence　of　different　nitrogen　sources　on　functional　bacteria　and　aerobic　denitrification　characteristics　in　oxygen-rich　condition　were　studied.　The　results　show　that　the　concentrations　for　total　nitrogen　and　ammonia　nitrogen　were　gradually　decreasing,　while　nitrite　or　nitrate　were　not　accumulated　during　the　process　under　25-30℃　together　with　a　high　dissolved　oxygen　concentration　(4.0~6.0　mg·L-1).　Both　nitrification　and　aerobic　denitrification　were　achieved　in　the　wastewater　with　a　low　C/N　ratio.　In　addition,　nitrite　and　nitrate　as　main　nitrogen　sources　were　respectively　used　to　confirm　the　function　of　aerobic　denitrifier.　The　results　show　the　removal　efficiency　of　nitrite　and　nitrate　within　18　h　under　high　temperature　(>40℃)　was　nearly　100%.　Therefore,　the　aerobic　denitrifier　in　aerobic　flow-separation　biofilm　was　proved　to　effectively　remove　ammonium　from　different　nitrogenous　effluents,　while　it　could　adapt　to　high　temperature　environment.　Comparing　with　the　pure　cultures,　the　functional　flora　as　object　was　more　superior　in　engineering　practice.　©　2016,　Science　Press.　All　right　reserved.