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In order to improve the quality of reclaimed water, a comparative study was conducted under different C/N and HRT conditions to examine the effect on advanced nitrogen and phosphorus removal by using sponge iron/sulfur composite fillers and low electrical current. The results indicated that both the sponge iron/sulfur composite fillers and low electrical current treatment can strengthen the removal efficiency of nitrogen and phosphorus, and their combination can further stabilize the pH value of 7.2~8.5 in the denitrification system. It was found that the removal of total nitrogen (TN) was mainly depended on the process of heterotrophic denitrification, hydrogen autotrophic denitrification and sulfur autotrophic denitrification, while 94.04% of the total phosphorus (TP) were removed in the form of iron-phosphate precipitation. Furthermore, biofilm was taken from the fillers to build the bacterial 16S rRNA gene clone library by adopting the high-throughput sequencing technologies. The results showed that the bacteria which can use both organic carbon and elemental hydrogen as its electron donor for denitrification accounted for 29.47% of the bacterial community in the sponge iron fillers system. The proportion of Thiobacillus bacteria which can use elemental sulfur as its electron donor reached 60.47% and 40.62% of the bacterial community in the system of sponge iron/sulfur composite fillers and the combined system of composite fillers with low electrical current, respectively. Therefore, there are obvious advantages of employing sponge iron/sulfur composite fillers and low electrical current to enhance the effect of advanced nitrogen and phosphorus removal from reclaimed water. © 2016, Chinese Society for Environmental Sciences. All right reserved.
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