A　rapid　and　quantitative　method　for　the　determination　of　N-6-Benzylademine　(N-6-BA)　was　established　through　the　application　of　surface-enhanced　Raman　spectroscopy　(SERS).　The　Raman　peak　intensities　of　N-6-BA　at　1002cm(-1)　positively　correlated　to　N-6-BA　concentrations　in　sprout　extracts.　The　R-2　reached　0.99,　and　RSDs　calculated　below　10%　at　the　concentration　range　of　0.1　approximate　to　5g　mL(-1).　The　average　recoveries　were　80.0%　approximate　to　98.2%　for　blank　samples　intentionally　contaminated　at　differing　levels　of　0.04,　0.4,　and　1g　g(-1).　The　whole　procedure,　including　sample　preparation　and　SERS　detection,　did　not　exceed　30min　for　a　set　of　6　samples.　This　study　indicates　that　SERS　is　a　promising　technique　for　rapid　tracing　analysis　and　on-site　testing　of　N-6-BA.