Subcellular localization of APOBEC3G by confocal laser scanning microscope (CLSM) - 文章详情页

作者：

Yang, Y.S. (Yang, Y.S..) | Li, L. (Li, L..) | Li, Z.L. (Li, Z.L..) | Zeng, Y. (Zeng, Y..)

收录：

Scopus PKU CSCD

摘要：

Apolipoprotein　B　mRNA-editing　enzyme　catalytic　polypeptide-like　3G　(APOBEC3G)　cDNA　was　amplified　from　total　RNA　prepared　from　nonpermissive　H9　cells　by　RT-PCR.　APOBEC3G　cDNA　is　1155nt　long,　encoding　384　amino　acids.　The　APOBEC3G　gene　was　then　cloned　into　the　eukaryotic　expression　vector　pEGFP-C3.　The　generated　pEGFP-3G　construct　was　then　transfected　into　CD4+　HeLa　cell　to　determine　the　expression　and　the　subcellular　localization　of　GFP-APOBEC3G　fusion　protein.　Under　CLSM　the　localization　of　the　expressed　GFP-APOBEC3G　in　the　cytoplasm　of　CD4+　HeLa　cells　was　observed.

关键词：

作者机构：

[ 1 ] [Yang, Y.S.]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100022, China
[ 2 ] [Li, L.]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100022, China
[ 3 ] [Li, Z.L.]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100022, China
[ 4 ] [Zeng, Y.]College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100022, China

通讯作者信息：

电子邮件地址：

yishu-y@bjut.edu.cn

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