We　present　an　attractive　model　of　surface-enhanced　Raman　scattering-based　lateral　flow　immunoassay　(SERS-LFIA)　for　the　sensitive　and　accurate　detection　of　Mycoplasma　pneumoniae　(MP)　infection　in　human　serum.　The　SERS-LFIA　strip　uses　Au@Ag　nanoparticles　(Au@Ag　NPs)　loaded　with　two　layers　of　Raman　dye　5,5-dithiobis-(2-nitrobenzoic　acid)　(DTNB)　as　SERS　tags.　The　advantages　of　the　dual　dye-loaded　SERS　tags　(Au/DTNB@Ag/DTNB)　are　the　high　sensitivity　and　the　bioconjugation　flexibility　of　the　detection　antibody.　As　determined　from　our　SERS-LFIA　strip,　human　IgM　was　quantified　by　monitoring　the　SERS　signal　on　the　test　line.　The　limit　of　detection　for　human　IgM　was　0.1　ng　mL(-1),　which　was　100　times　more　sensitive　than　that　by　using　the　colorimetric　method.　Our　assay　results　for　20　MP-specific　IgM　positive　serum　specimens　showed　100%　accuracy　and　detection　rate,　whereas　the　parallel　enzyme-linked　immunosorbent　assay　only　showed　85%　detection　rate.　The　SERS-LFIA　strip　also　exhibited　high　specificity　and　potential　clinical　applications.　Therefore,　our　SERS-based　LFIA　strip　has　strong　potential　for　practical　applications　in　the　sensitive　and　rapid　detection　of　MP.