Glycidoxypropyl-functionalized　magnetic　mesoporous　SiO2/Fe3O4　hollow　microspheres　(FMMS)　with　various　amounts　of　terminally　bonded　groups　in　the　pore　channels　were　synthesized　by　the　co-condensation　of　tetraethoxysilane　(TEOS)　and　3-glycidoxypropyltrimethoxylsilane　(GPTMS)　in　the　presence　of　cetyltrimethylammonium　bromide　and　1,3,5-triisoporpylbenzene,　and　utilized　as　supports　for　laccase　immobilization.　It　is　revealed　that　glycidoxypropyl　groups　have　been　covalently　attached　to　the　pore　wall　of　magnetic　mesoporous　SiO2/Fe3O4　hollow　microspheres　(MMS).　The　magnetic　mesoporous　SiO2/Fe3O4　microspheres　(sample10%　FMMS)　have　a　saturation　magnetization　of　13.6　emu.g-1　a　low　coercivity　(50　Oe).　The　functional　materials　preserve　a　mesoscopic　ordering　at　a　concentration　of　GPTMS　as　high　as　15%.　The　BET　surface　area　and　pore　volume　of　the　functionalized　materials　decrease　with　increasing　amount　of　GPTMS,　but　a　desirable　pore　structure　remains　when　the　GPTMS　amount　increases　to　10%.　The　10%　FMMS　material　exhibits　higher　laccase　immobilization　and　stability　than　pure　MMS　because　of　the　covalent　interaction　between　the　amino　groups　of　laccase　and　the　epoxy　groups　of　the　functional　MMS.　©　2016　Trans　Tech　Publications,　Switzerland.