Shear　force　is　of　significance　for　culturing　stem　cells　on　the　biochip,　which　depends　mainly　on　velocity　gradient　of　the　culture　medium.　Based　on　fluid　dynamics,　eight　types　of　culturing　areas　were　studied　using　finite　element　method　and　realizable　κ-ε　model　in　this　paper.　Distribution　of　velocity　in　culturing　area　and　shear　force　in　the　center　area　were　obtained.　By　comparing　analysis　results,　one　was　selected　from　eight　types　of　culturing　areas　according　to　the　requirements　of　minimizing　shear　force　and　remaining　less　old　medium,　which　were　fitful　for　providing　medium　for　stem　cells　on　microfluidic　chip.　According　to　the　results,　two　pieces　of　design　experience　were　summed　up.