EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humoral protective response against EV71 lethal challenge Microbiology - 文章详情页

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Scopus PubMed

摘要：

Background:　Enterovirus　71　(EV71)　is　the　most　common　causative　pathogens　of　hand,　foot　and　mouth　disease　(HFMD)　associated　with　severe　neurological　complications.　There　is　a　great　need　to　develop　prophylactic　vaccine　against　EV71　infection.　Results:　EV71　virus-like　particle　(VLP)　was　produced　in　yeast　expression　system　by　the　co-expression　of　four　EV71　structural　proteins　VP1-VP4.　Immunization　with　the　recombinant　VLPs　elicited　potent　anti-EV71　antibody　responses　in　adult　mice　and　anti-VLP　sera　were　able　to　neutralize　EV71　virus　in　vitro.　Neonatal　mice　model　demonstrated　VLP　immunization　conferred　protection　to　suckling　mice　against　the　lethal　viral　challenge.　Conclusions:　Co-expression　of　four　EV71　structural　proteins　VP1-VP4　in　yeast　expression　systems　is　an　effective　method　to　produce　EV71　VLPs.　VLP-based　vaccine　shows　great　potential　to　prevent　EV71　infection.　©　2016　Wang　et　al.

关键词：

Enterovirus 71; Hand foot and mouth disease; Vaccine; Virus-like particle; Yeast

作者机构：

[ 1 ] [Wang, X.]College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, China
[ 2 ] [Xiao, X.]College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, China
[ 3 ] [Zhao, M.]College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, China
[ 4 ] [Liu, W.]College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, China
[ 5 ] [Pang, L.]Department of Neurology, Beijing Ditan Hospital, Capital Medical University, Beijing, China
[ 6 ] [Sun, X.]Research Center for Life Science, Beihua University, Jilin, China
[ 7 ] [Cen, S.]Department of Virology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Science, Beijing, China
[ 8 ] [Yang, B.B.]Department of Laboratory Medicine and Pathobiology, University of Toronto, 2075 Bayview Avenue, Toronto, M4N 3M5, Canada
[ 9 ] [Huang, Y.]Department of Neurology, Beijing Ditan Hospital, Capital Medical University, Beijing, China
[ 10 ] [Sheng, W.]College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, China
[ 11 ] [Zeng, Y.]College of Life Science and Bioengineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing, China

通讯作者信息：

黄艳
[Huang, Y.]Department of Neurology, Beijing Ditan Hospital, Capital Medical UniversityChina

电子邮件地址：

hyming@medmail.com.cn

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