Transforming activity of a novel mutant of HPV16 E6E7 fusion gene - 文章详情页

作者：

Xie, Q. (Xie, Q..) | Zhou, Z.-X. (Zhou, Z.-X..) (学者：周志祥) | Li, Z.-L. (Li, Z.-L..) | Zeng, Y. (Zeng, Y..)

收录：

Scopus PubMed CSCD

摘要：

An　optimized　recombinant　HPV16　E6E7　fusion　gene　(HPV16　ofE6E7)　was　constructed　according　to　codon　usage　for　mammalian　cell　expression,　and　a　mutant　of　HPV16　ofE6E7　fusion　gene　(HPV16　omfE6E7)　was　generated　by　site-directed　mutagenesis　at　L57G,　C113R　for　the　E6　protein　and　C24G,　E26G　for　the　E7　protein　for　HPV16　ofE6E7　[patent　pending　(CN　101100672)].　The　HPV16　omfE6E7　gene　constructed　in　this　work　not　only　lost　the　transformation　capability　to　NIH　3T3　cells　and　tumorigenicity　in　SCID　mice,　but　also　maintained　very　good　stability　and　antigenicity.　These　results　suggests　that　the　HPV16　omfE6E7　gene　should　undergo　further　study　for　application　as　a　safe　antigen-specific　therapeutic　vaccine　for　HPV16-associated　tumors.　©　2011　Wuhan　Institute　of　Virology,　CAS　and　Springer-Verlag　Berlin　Heidelberg.

关键词：

E6; E7; Gene mutation; Gene optimization; Human papillomavirus (HPV); Transformation

作者机构：

[ 1 ] [Xie, Q.]College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
[ 2 ] [Zhou, Z.-X.]College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
[ 3 ] [Li, Z.-L.]College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
[ 4 ] [Zeng, Y.]College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China

通讯作者信息：

周志祥
[Zhou, Z.-X.]College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China

电子邮件地址：

zhouzhixiang@bjut.edu.cn

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