Background:　This　study　was　aimed　at　developing　a　myocardial　bridge-mural　coronary　artery　simulative　device　and　analyzing　the　relationship　between　shear　stress　on　the　mural　coronary　artery　and　atherosclerosis.　Methods:　A　myocardial　bridge-mural　coronary　artery　simulative　device　was　used　to　simulate　experiments　in　vitro.　In　the　condition　of　maintaining　any　related　parameters　such　as　system　temperature,　average　flow　rate,　and　heart　rate,　we　calculated　and　observed　changes　in　proximal　and　distal　mean　values,　and　oscillatory　value　of　shear　stress　on　the　mural　coronary　artery　by　regulating　the　compression　level　of　the　myocardial　bridge　to　the　mural　coronary　artery.　Results:　Under　0%　compression,　no　significant　differences　were　observed　in　distal　and　proximal　mean　values　and　oscillatory　value　of　the　shear　stress　on　the　mural　coronary　artery.　With　the　increase　in　the　degree　of　compression,　the　mean　shear　stress　at　the　distal　end　was　greater　than　that　at　the　proximal　end,　but　the　oscillatory　value　of　the　shear　stress　at　the　proximal　end　was　greater　than　that　at　the　distal　end.　Conclusions:　The　experimental　results　of　this　study　indicate　that　myocardial　bridge　compression　leads　to　abnormal　hemodynamics　at　the　proximal　end　of　the　mural　coronary　artery.　This　abnormal　phenomenon　is　of　great　significance　in　the　study　of　atherosclerosis　hemodynamic　pathogenesis,　which　has　potential　clinical　value　for　pathological　effects　and　treatments　of　myocardial　bridge.