Based　on　fluid　dynamics,　17　types　of　air　chambers　are　studied　using　finite　element　method　and　Realizable　κ-ε　model　in　this　paper.　By　comparing　analysis　results,　two　are　selected　from　17　types　of　air　chambers　according　to　changing　air　quickly　and　remaining　less　old　air,　which　are　fitful　for　providing　air　for　stem　cells　on　microfluidic　chip.　Two　experiences　are　obtained　from　the　analysis.　In　practical　terms,　appropriate　improvement　is　made　for　remaining　less　old　air　further.　Results　show　that　the　chosen　and　improved　chamber　is　suitable　for　using　microfluidic　chip　to　culture　stem　cells.