All　over　the　world,　cancer　has　become　a　major　public　health　problem.　Epidemiological　studies　have　shown　that　about　80%-90%　of　human　cancers　are　caused　by　the　environment;　the　most　important　cancer-causing　factors　are　chemical　factors.　Di-region　theory,　one　mechanism　of　chemical　carcinogens,　pointed　out　that　environmental　carcinogenic　agents　were　metabolized　into　a　specific　bi-functional　alkylation　agent,　then　induced　DNA　interstrand　cross-linking　and　final　caused　cancer.　Although　there　are　many　methods　to　detect　DNA　interstrand　cross-linking,　its　mechanism　that　causes　normal　cells　to　become　tumor　is　still　unclearly,　and　has　also　become　the　focus　of　attention.　Based　on　the　structure,　formation　process　and　detection　methods　of　DNA　interstrand　cross-linking,　the　research　progress　on　the　relationship　of　DNA　interstrand　cross-linking　and　cancer　was　reviewed　in　this　paper.　It　hopes　to　provide　some　ideas　for　the　research　on　the　mechanism　of　environmental　carcinogens.