We　examined　the　expression　of　vascular　endothelial　growth　factor　(VEGF)　and　characteristics　of　human　neural　stem　cells　after　transfection　with　the　lentiviral　vector　encoding　the　VEGF　165　gene.　The　latter　gene　was　amplified　from　the　human　breast　cancer　cell　line　MCF-7　using　RT-PCR;　then　the　target　gene　was　cloned　into　the　pCDH-CMV-MCS-EF1-copGFP,　a　lentiviral　expressing　plasmid.　After　transformation,　enzyme　digestion　led　to　a　correct　length　of　the　VEGF165　gene,　and　DNA　sequencing　analysis　confirmed　that　the　VEGF165　gene　sequence　was　exactly　the　same　as　that　reported　by　the　GeneBank.　Then,　the　recombinant　lentivirus　produced　by　293T　cells　and　packaging　plasmids　were　transfected　into　fourthpassage　human　neural　stem　cells　(NSCs).　One　week　after　transfection　with　pCDH-VEGF165,　NSCs　expressed　VEGF　stably,　and　their　proliferation　ability　significantly　increased.　Furthermore,　human　NSCs　kept　their　characteristics　and　multiple　differentiation　activity　after　transfection.　Our　results　indicate　that　human　NSCs　can　express　vascular　endothelial　growth　factor　highly　and　stably　via　transfection　with　the　lentiviral　vector　encoding　the　VEGF165　gene,　which　may　be　useful　for　future　research　on　function　recovery　after　stroke.