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Volatile fatty acids(VFAs) are suitable carbon sources for nitrogen and phosphorus removal. A large amount of VFAs can be produced in the alkaline fermentation process of WAS. Temperature and sludge concentration are two critical factors for waste activated sludge (WAS) fermentation, therefore the effect of temperature and sludge concentration on hydrolysis and acidification of WAS was investigated at 15°C, 35°C and pH=10 under anaerobic condition. The results showed that soluble chemical oxygen demand (SCOD) production yields were impacted little by sludge concentration. Low or high sludge concentrations were disadvantage for acid production, the best sludge concentration for acid production was VSS=8.54 g/L. At any sludge concentration investigated, acetic acid dominated in the six volatile short chain fatty acids produced by sludge fermentation, besides the percentage of acetic acids were higher at low sludge concentration than high sludge concentration. Temperature impacted significantly on the hydrolysis of WAS with higher sludge concentration, but it impacted significantly on the acidification of WAS with lower sludge concentration. Medium sludge concentration at 6.832, 8.540 g/L influenced little on ammonia release at 15°C and 35°C, sludge concentration influenced more significantly on phosphorus release at 35°C than at 15°C.
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