In　order　to　explore　the　relationship　between　human　papilloma　virus　(　HPV)　and　upper　gastrointestinal　cancer(esophageal　cancer),　An　esophageal　squamous　cell　carcinoma(ESCC)　tissue　was　obtained　from　a　76　year　old　Chinese　female　patient　from　Anyang　city,　a　high-incidence　area　for　esophageal　cancer,　in　China.　Transplanted　tumor　was　formed　through　direct　SCID　mouse　tumorigenicity　experiment　and　cultured　monolayer　cells　were　obtained　after　several　passages　and　screenings　Immunofluorescence　test,　cell　growth　curve,　soft　agar　assay,　chromosome　analysis　and　tissues　HE　staining　were　also　performed　to　confirm　the　epithelial　cell　origin.　Cell　DNA　STR　typing　results　showed　that　no　three　alleles　was　observed,indicating　no　contamination　of　human　cells.　DNA　analysis　revealed　the　presence　of　HPV　type　18　DNA　in　this　cell　line.　DOLINK　test　found　the　E6　protein　expression　of　HPV　virus.　We　concluded　that　the　established　cell　line　is　a　new　esophageal　squamous　cell-origincarcinoma　cell　line　with　HPV　DNA　positive　and　expression　of　viral　oncoprotein.　It　provides　new　cytologic　material　for　performing　etiology　studies　on　the　occurrence　and　development　of　esophageal　cancer.