UiO-66　analogues　are　good　candidates　as　stationary　phase　in　HPLC　because　of　their　chemical/thermal　stability,　large　surface　area,　and　two　cage　structures.　Here,　two　UiO-66　analogues,　UiO-66-NH2　and　UiO-67,　were　synthesized　and　used　as　stationary　phase　in　HPLC　to　evaluate　their　performance　in　the　separation　of　substituted　benzenes　(SBs)　and　polycyclic　aromatic　hydrocarbons　(PAHs).　The　results　showed　that　SBs　could　be　well　separated　on　UiO-66-NH2　column　but　not　on　UiO-67　column.　Nonetheless,　PAHs　could　be　well　separated　on　UiO-67　column.　The　separation　mechanisms　of　SBs　and　PAHs　on　UiO-66　analogues　may　be　involved　in　the　pore　size　and　functional　group　in　the　frameworks　of　UiO-66　analogues.　Introduction　of　the-NH2　into　UiO-66　significantly　reduced　its　adsorption　capacity　for　SB　congeners,　which　resulted　in　less　separation　of　SBs　on　UiO-66-NH2.　As　for　the　separation　of　PAHs　on　UiO-67　column,　the　Pi-Pi　stacking　effect　was　supposed　to　play　a　vital　role.