The　common　methods　of　detection　deoxynivalenol　(DON)　are　limited　to　time-consuming　or　lower　sensitivity.　The　carboxyl　modification　of　core/shell　structure　up-converting　nanoparticles　was　produced　and　conjugated　to　anti-DON　antibodies,　and　then　applied　in　lateral　flow　strip　to　detection　DON.　The　detection　of　limitation　in　cornmeal　was　0.7　ng/mL.　The　50%　of　inhibit　concentration　was　9　ng/mL.　The　detection　range　was　from　0.7　to　50　ng/mL　and　the　recovery　rates　were　from　90%　to　156　%.　The　study　has　developed　method　for　detecting　DON　rapidly　and　sensitively　based　on　up-converting　immunochromatographic　technology,　which　can　also　provided　reference　to　the　detection　of　veterinary　residues.　©　2015,　Beijing　University　of　Technology.　All　right　reserved.