BACKGROUND:　Placenta　is　a　valuable　source　of　mesenchymal　stem　cells　for　stem　cell　therapy　and　future　application　in　the　field　of　regenerative　medicine.　However,　conventional　methods　cannot　acquire　a　large　amount　of　purified　human　placenta-derived　mesenchymal　stem　cells.　Here,　we　present　a　new　method　for　isolating　human　placenta-derived　mesenchymal　stem　cells　suitable　for　banking　strategies　and　for　future　clinical　applications.　OBJECTIVE:　To　analyze　the　biological　characteristics　of　human　placenta-derived　mesenchymal　stem　cells　cultured　by　tissue　dissociating　and　collagenase　digestion.　METHODS:　Human　placenta-derived　mesenchymal　stem　cells　were　obtained　from　human　placenta　by　tissue　dissociating　and　collagenase　digestion　method.　Immunophenotype　was　analyzed　by　flow　cytometry.　Growth　curve　was　determined　by　MTT　assay,　and　differentiation　ability　was　evaluated　by　in　vitro　adipogenic,　osteogenic　and　chondrogenic　induction　as　well.　RESULTS　AND　CONCLUSION:　Human　placenta-derived　mesenchymal　stem　cells　could　be　passaged　stably　in　vitro.　Furthermore,　the　cells　expressed　CD73,　CD90,　CD105,　but　were　negative　for　the　markers　of　CD11b,　CD19,　CD34,　CD45,　and　HLA-DR.　Human　placenta-derived　mesenchymal　stem　cells　proliferated　actively　and　began　to　grow　logarithmically　at　days　3-5　followed　by　a　plateau　period　at　day　6.　In　addition,　the　isolated　cells　could　be　induced　into　adipocytes,　osteocytes,　chondrocytes　in　vitro.　In　a　word,　the　results　of　this　study　demonstrated　that　the　tissue　dissociating　and　collagenase　digestion　method　is　an　efficient　method　for　obtaining　a　large　amount　of　human　placenta-derived　mesenchymal　stem　cells　that　can　be　stably　cultured　in　vitro　and　have　strong　proliferative　ability.　©　2015,　Journal　of　Clinical　Rehabilitative　Tissue　Engineering　Research.　All　rights　reserved.