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The bacterial diversity in an anoxic/aerobic membrane bioreactor process (A/O-MBR) was studied by 16S rDNA cloning and sequencing method. The experimental results showed that the clone library of aerobic sludge sample comprised 9 major groups. The largest fractions were Proteobacteria and Bacteroidetes, which accounted for 37.04% and 14.81% of the total clones, respectively. The protions of Acidobacteria, uncultured bacterium, Planctomycetes, Chlorobi and uncultured Chloroflexi bacterium were 11.11%, 11.11%, 5.56%, 7.41% and 7.41%, respectively. The relatively lower portions were 1.85% Nitrospirae and 1.85% Euglenozoa. The clone library of anoxic sludge sample was found to include 10 major groups, the largest fractions were Proteobacteria, Bacteroidetes and uncultured bacterium, which accounted for 27.91%, 13.95% and 12.79%, respectively. The protions of Planctomycetes, Acidobacteria and Chloroflexi in this library were 9.3%, 9.3% and 9.3%, respectively. The relatively lower portions were 6.98% Nitrospirae,8.14% Euglenozoa,1.16% Gemmatimonadetes and 1.16% Actinobacteria. The main bacteria groups were similar in the two pools, but the species and proportions were different. Proteobacteria was identified to be the main denitrifer genera in the system. ©, 2015, Science Press. All right reserved.
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