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作者:

Zheng, L. (Zheng, L..) | Li, J. (Li, J..) | Ren, J. (Ren, J..) | Hou, A. (Hou, A..) | Zheng, Z. (Zheng, Z..)

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摘要:

The bacterial diversity in an anoxic/aerobic membrane bioreactor process (A/O-MBR) was studied by 16S rDNA cloning and sequencing method. The experimental results showed that the clone library of aerobic sludge sample comprised 9 major groups. The largest fractions were Proteobacteria and Bacteroidetes, which accounted for 37.04% and 14.81% of the total clones, respectively. The protions of Acidobacteria, uncultured bacterium, Planctomycetes, Chlorobi and uncultured Chloroflexi bacterium were 11.11%, 11.11%, 5.56%, 7.41% and 7.41%, respectively. The relatively lower portions were 1.85% Nitrospirae and 1.85% Euglenozoa. The clone library of anoxic sludge sample was found to include 10 major groups, the largest fractions were Proteobacteria, Bacteroidetes and uncultured bacterium, which accounted for 27.91%, 13.95% and 12.79%, respectively. The protions of Planctomycetes, Acidobacteria and Chloroflexi in this library were 9.3%, 9.3% and 9.3%, respectively. The relatively lower portions were 6.98% Nitrospirae,8.14% Euglenozoa,1.16% Gemmatimonadetes and 1.16% Actinobacteria. The main bacteria groups were similar in the two pools, but the species and proportions were different. Proteobacteria was identified to be the main denitrifer genera in the system. ©, 2015, Science Press. All right reserved.

关键词:

16S rDNA clone library; AO-MBR; Bacterial diversity

作者机构:

  • [ 1 ] [Zheng, L.]College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, 100124, China
  • [ 2 ] [Li, J.]College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, 100124, China
  • [ 3 ] [Ren, J.]College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, 100124, China
  • [ 4 ] [Hou, A.]College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, 100124, China
  • [ 5 ] [Zheng, Z.]College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, 100124, China

通讯作者信息:

  • [Li, J.]College of Architecture and Civil Engineering, Beijing University of TechnologyChina

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来源 :

Chinese Journal of Environmental Engineering

ISSN: 1673-9108

年份: 2015

期: 5

卷: 9

页码: 2503-2509

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