A　novel　DEAMOX　system　was　developed　for　nitrogen　removal　from　domestic　wastewater　and　nitrate　(NO3--N)　sewage　in　sequencing　batch　reactor　(SBR).　High　nitrite　(NO2--N)　was　produced　from　NO3--N　reduction　in　partial-denitrification　process,　which　served　as　electron　acceptor　for　anammox　and　was　removed　with　ammonia　(NH4--N)　in　domestic　wastewater　simultaneously.　A　500-days　operation　demonstrated　that　the　efficient　and　stable　nitrogen　removal　performance　could　be　achieved　by　DEAMOX.　The　total　nitrogen　(TN)　removal　efficiency　was　as　high　as　95.8%　with　influent　NH4--N　of　63.58　mg　L-1　and　NO3--N　of　69.24　mg　L-1.　The　maximum　NH4--N　removal　efficiency　reached　up　to　94.7%,　corresponding　to　the　NO3--N　removal　efficiency　of　97.8%.　The　biomass　of　partial-denitrification　and　anammox　bacteria　was　observed　to　be　wall-growth.　The　deteriorated　nitrogen　removal　performance　occurred　due　to　excess　denitrifying　microbial　growth　in　the　outer　layer　of　sludge　consortium,　which　prevented　the　substrate　transfer　for　anammox　inside.　However,　an　excellent　nitrogen　removal　could　be　guaranteed　by　scrapping　the　superficial　denitrifying　biomass　at　regular　intervals.　Furthermore,　the　high-throughput　sequencing　analysis　revealed　that　the　Thauera　genera　(26.33%)　was　possibly　responsible　for　the　high　NO2--N　accumulation　in　partial-denitrification　and　Candidatus　Brocadia　(1.7%)　was　the　major　anammox　species.　(C)　2017　Elsevier　Ltd.　All　rights　reserved.