An　ultra-high　performance　liquid　chroniatography-tandein　mass　spectrometry　(UPLC-MS/MS)　method　with　molecularly　imprinted　solid　phase　extraction　for　the　determina-tion　of　five　β2-gonists　residues　in　pork　has　been　developed.　After　the　sample　preparation,　the　ammonium　acetate/acetic　acid　buffer　was　added.　followed　by　the　internal　standard　and　β-glu-curonidase/arylsidfat　ase　enzyme.　The　solution　was　incubated　at　55　°C　for　2　h.　After　adjusting　the　pH　of　the　solution,　it　was　purified　by　a　molecularly　imprinted　solid　phase　extraction　col-umn,　then　analyzed　on　a　BEH　C18　column　with　inethanol-0.1%　(v/v)　formic　acid　aqueous　solution　as　the　mobile　phases　in　gradient　elution　mode.　The　MS/MS　analysis　was　in　positive　ion　mode　and　multiple　reaction　monitoring　modi1.　The　analytcs　were　quantified　by　the　internal　standard　method.　The　limits　of　detection　(LODs,　S/N=3)　and　the　limits　of　quantification　(LOQs,　S/N=　10)　were　0.005-0.009　μg/kg　and　0.　015-0.　025　μg/kg,　respectively,　hi　the　range　of　0-10　μg/kg,　the　correlation　coefficients　of　linear　calibration　curves　were　not　less　than　0.　993　3.　At　the　spiked　levels　of　0.　25.　1.　0　and　5.　0　μg/kg,　the　recoveries　were　80.　4%-92.　9%　with　the　relative　standard　deviations　of　1.3%-6.　3%.　The　method　is　of　high　sensitivity,　good　reproducibility,　high　recovery,　and　is　useful　for　the　simultaneous　determination　of　multiple　β2-agonists　residues.