The　aim　of　the　present　work　was　to　evaluate　the　effects　of　carbon　sources　and　chemical　oxygen　demand　(COD)/NO2--N　ratios　on　the　anammox-denitrification　coupling　process　of　the　simultaneous　partial　nitrification,　anammox　and　denitrification　(SNAD)　biofilm.　Also,　the　anammox　activities　of　the　SNAD　biofilm　were　investigated　under　different　temperature.　Kaldnes　rings　taken　from　the　SNAD　biofilm　reactor　were　operated　in　batch　tests　to　determine　the　nitrogen　removal　rates.　As　a　result,　with　the　carbon　source　of　sodium　acetate,　the　appropriate　(COD)/NO2--N　ratios　for　the　anammox-denitrification　coupling　process　were　1　and　2.　With　the　(COD)/NO2--N　ratios　of　1,　2,　3,　4　and　5,　the　corresponding　NO2--N　consumption　via　anammox　was　87.1%,　52.2%,　29.3%,　23.7%　and　16.3%,　respectively.　However,　with　the　carbon　source　of　sodium　propionate　and　glucose,　the　anammox　bacteria　was　found　to　perform　higher　nitrite　competitive　ability　than　denitrifiers　at　the　(COD)/NO2--N　ratio　of　5.　Also,　the　SNAD　biofilm　could　perform　anammox　activity　at　15　degrees　C　with　the　nitrogen　removal　rate　of　0.071　kg　total　inorganic　nitrogen　per　kg　volatile　suspended　solids　per　day.　These　results　indicated　that　the　SNAD　biofilm　process　might　be　feasible　for　the　treatment　of　municipal　wastewater　at　normal　temperature.