Single　or　few-layer　nanosheets　of　MoS2　(MoS2　nanosheets)　and　a　composite　composed　of　MoS2　nanosheets,　Au　nanoparticles　(AuNPs)　and　hemin　(HE)　(denoted　as　MoS2-Au-HE)　were　prepared.　The　composites　possessed　high　synergetic　catalysis　activity　towards　the　electroreduction　of　hydrogen　peroxide.　Furthermore,　glucose　oxidase　(GOD)　and　AuNPs　were　used　as　marker　of　the　complementary　DNA　(cDNA)　strand　of　kanamycin　aptamer　to　prepare　a　conjugate　(reffered　as　cDNA-Au-GOD)　that　was　designed　as　the　signal　probe.　Both　cDNA-Au-GOD　and　MoS2-Au-HE　were　applied　to　fabricate　aptasensor　for　kanamycin.　MoS2-Au-HE　acted　as　solid　platform　for　kanamycin　aptamer　and　signal　transmitters.　AuNPs　were　employed　as　the　supporter　of　cDNA　and　GOD　which　catalyze　dissolved　oxygen　to　produce　hydrogen　peroxide　in　the　presence　of　glucose.　Then　cathodic　peak　current　of　H2O2　was　recorded　by　differential　pulse　voltammetry　(DPV).　The　electrochemical　reduction　of　H2O2　was　catalyzed　by　MoS2-Au-HE　that　was　modified　onto　the　surface　of　a　glassy　carbon　electrode　(GCE).　The　cathodic　peak　current　of　H2O2　was　highly　linearly　decreased　with　an　increase　of　kanamycin　concentrations　from　1.0　ng/L　to　1.0x10(5)　ng/L,　with　a　detection　limit　of　0.8　ng/L.　This　aptasensor　can　be　used　to　detect　kanamycin　in　milk　with　high　specificity,　sensitivity　and　selectivity.