In　order　to　solve　the　problem　of　low　adenovirus-transfected　efficiency　on　cancer　cells　of　tumor　gene　therapy,　efficient　and　low-toxicity　transfection　reagents　have　been　considered　as　a　promising　method.　In　this　research,　n-Dodecyl-β-D-Maltoside　(DDM),　DC-Chol　and　SDS　were　affected　synergistically　with　eGFP-Ad　to　transfect　HepG2,　EC109,　L-02　and　Het-1A　cells.　The　drug　effects　were　evaluated　by　detection　and　comparison　of　the　green　fluorescence　expression　after　48　hours　of　adenovirus　transfection.　The　data　showed　that　the　transfection　efficiency　of　DDM,　DC-Chol,　and　SDS　was　significantly　improved　in　HepG2　and　EC109　cancer　cells　when　comparing　to　L-02　and　Het-1A　human　normal　cells.　The　incremental　transfect　efficiencies　of　DDM　(3　μg/mL),　DC-Chol　(1　000　μg/mL)　and　SDS(0.5　μg/mL)　on　tumor　cells　HepG2　and　EC109,　at　about　85%　and　78%,　80%　and　76%,　45%　and　41%,　were　16　times　and　14.　6　times,　15　times　and　14.　2　times,　8　times　and　7.　2　times　as　great　as　the　non-treated　groups.　The　results　indicated　that　DDM　significantly　improved　the　transfection　efficiency　of　adenovirus　on　tumor　cells,　which　can　provide　the　technical　reference　for　adenovirus-mediated　gene　therapy.　©　2017,　Editorial　Department　of　Journal　of　Beijing　University　of　Technology.　All　right　reserved.