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学者姓名：高景峰
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摘要 ：
Phenacetin (PNCT), a common antipyretic and analgesic drug, is often used to treat fever and headache. However, the effect of PNCT on nitrifiers in wastewater treatment processes remains unclear. The practicability of attaining partial nitrification (PN) through inhibitor-PNCT was investigated in this study. The optimal treatment conditions of soaking once for 18 h with 2.50 x 10(-3) g PNCT/(g MLSS) were applied to the PN stability experiment. The results showed that ammonia oxidation activity recovered quickly after 3 cycles of operation, while nitrite oxidation activity was suppressed steadily. In addition, average ammonium removal efficiency and nitrite accumulation ratio during 138 cycles could reach 94.94% and 85.38%, respectively. Complimentary DNA high-throughput sequencing and oligotyping analysis showed that the activity of Nitrosomonas would gradually surpass Nitrospira after PNCT treatment only once. The decrease of Nitrospira activity was accompanied by the simplification of oligotypes after PNCT treatment, while Nitrosomonas could adapt to PNCT stress by reducing the differences between oligotypes. Metagenomics revealed that the decrease in the number of NXR in the nitrogen metabolism pathways was the key reason for achieving PN. The potential mechanisms might be that the dominant nitrite-oxidizing bacteria and complete ammonia oxidizers were bio-killed by PNCT. (C) 2021 Elsevier B.V. All rights reserved.
关键词 ：
cDNA high-throughput sequencing cDNA high-throughput sequencing Metagenomics Metagenomics Nitrogen metabolism pathways Nitrogen metabolism pathways Oligotyping analysis Oligotyping analysis Partial nitrification Partial nitrification Phenacetin Phenacetin
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| GB/T 7714 | Wu, Zejie , Gao, Jingfeng , Cui, Yingchao et al. Metagenomics insights into the selective inhibition of NOB and comammox by phenacetin: Transcriptional activity, nitrogen metabolism and mechanistic understanding [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
| MLA | Wu, Zejie et al. "Metagenomics insights into the selective inhibition of NOB and comammox by phenacetin: Transcriptional activity, nitrogen metabolism and mechanistic understanding" . | SCIENCE OF THE TOTAL ENVIRONMENT 803 (2022) . |
| APA | Wu, Zejie , Gao, Jingfeng , Cui, Yingchao , Li, Dingchang , Dai, Huihui , Guo, Yi et al. Metagenomics insights into the selective inhibition of NOB and comammox by phenacetin: Transcriptional activity, nitrogen metabolism and mechanistic understanding . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
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摘要 ：
The abuse of antibiotics on animals could induce the development of antibiotic resistant genes (ARGs) and antibiotic resistant bacteria (ARB), and acesulfame potassium (ACE) is the widely used artificial sweetener in animal feed. Generally speaking, ACE and ARB often coexist in livestock wastewater, however, the impact of the co-occurrence of ACE and ARB on the transmission of ARGs is still unknown. In this study, the effects of ACE on vertical gene transfer (VGT) and horizontal gene transfer (HGT) were both evaluated. For VGT, ACE may hinder the spread of sul gene in Pseudomonas HLS-6 by blocking ARB growth. As for HGT (from Escherichia coli DH5 alpha to Pseudomonas HLS-6), environmentally relevant ACE concentration could facilitate the conjugative transfer. The underlying mechanisms of HGT were characterized by enhanced cell membrane permeability, reactive oxygen species overproduction, SOS response, energy supply, which were all further verified by the changes in transcription levels of related genes. Interestingly, intracellular Mg2+ in donor strain was found for the first time as an indicator for the conjugation occurrence in ACE treated mating system. This study may provide new insights into the role of ACE on ARGs proliferation and highlight its potential environmental impacts.
关键词 ：
Acesulfame potassium Acesulfame potassium Horizontal gene transfer Horizontal gene transfer Intracellular Mg2+ Intracellular Mg2+ Reactive oxygen species Reactive oxygen species Vertical gene transfer Vertical gene transfer
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| GB/T 7714 | Li, Ziqiao , Gao, Jingfeng , Guo, Yi et al. Enhancement of antibiotic resistance dissemination by artificial sweetener acesulfame potassium: Insights from cell membrane, enzyme, energy supply and transcriptomics [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2022 , 422 . |
| MLA | Li, Ziqiao et al. "Enhancement of antibiotic resistance dissemination by artificial sweetener acesulfame potassium: Insights from cell membrane, enzyme, energy supply and transcriptomics" . | JOURNAL OF HAZARDOUS MATERIALS 422 (2022) . |
| APA | Li, Ziqiao , Gao, Jingfeng , Guo, Yi , Cui, Yingchao , Wang, Yuwei , Duan, Wanjun et al. Enhancement of antibiotic resistance dissemination by artificial sweetener acesulfame potassium: Insights from cell membrane, enzyme, energy supply and transcriptomics . | JOURNAL OF HAZARDOUS MATERIALS , 2022 , 422 . |
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摘要 ：
Antibiotic resistant bacteria (ARB) and the antibiotic resistance genes (ARGs) dissemination via plasmid-mediated conjugation have attracted considerable attentions. In this research, sulfidated nanoscale zerovalent iron (S-nZVI)/peroxymonosulfate (PMS) and S-nZVI/peroxydisulfate (PDS) process were investigated to inactivate ARB (Escherichia coli DH5 alpha with RP4 plasmid, Pseudomonas. HLS-6 contains sul1 and intI1 on genome DNA sequence). S-nZVI/PMS system showed higher efficiency than S-nZVI/PDS on ARB inactivation. Thus, the optimal condition 28 mg/L S-nZVI coupled with 153.7 mg/L (0.5 mM) PMS was applied to remove both intracellular ARGs (iARGs) and ARB. The oxidative damage of ARB cell was systemically studied by cell viability, intracellular Mg2+ levels, the changes of extracellular and internal structure, integrity of cell walls and membranes and enzymatic activities. S-nZVI/PMS effectively inactivated ARB (similar to 7.32 log) within 15 min. These effects were greatly higher than those achieved individually. Moreover, removal efficiencies of iARGs sul1, intI1 and tetA were 1.52, 1.79 and 1.56 log, respectively. These results revealed that S-nZVI and PMS have a synergistic effect against ARB and iARGs. The regrowth assays illustrated that the ARB were effectively inactivated. By verifying the inhibitory impacts of S-nZVI/PMS treatment on conjugation transfer, this work highlights a promising alternative technique for inhibiting the horizontal gene transfer.
关键词 ：
Antibiotic resistant bacteria and genes Antibiotic resistant bacteria and genes Conjugative transfer Conjugative transfer Peroxydisulfate Peroxydisulfate Peroxymonosulfate Peroxymonosulfate Sulfidated nanoscale zerovalent iron Sulfidated nanoscale zerovalent iron
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| GB/T 7714 | Liu, Ying , Gao, Jingfeng , Wang, Yuwei et al. The removal of antibiotic resistant bacteria and genes and inhibition of the horizontal gene transfer by contrastive research on sulfidated nanoscale zerovalent iron activating peroxymonosulfate or peroxydisulfate [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2022 , 423 . |
| MLA | Liu, Ying et al. "The removal of antibiotic resistant bacteria and genes and inhibition of the horizontal gene transfer by contrastive research on sulfidated nanoscale zerovalent iron activating peroxymonosulfate or peroxydisulfate" . | JOURNAL OF HAZARDOUS MATERIALS 423 (2022) . |
| APA | Liu, Ying , Gao, Jingfeng , Wang, Yuwei , Duan, Wanjun , Liu, Jie , Zhang, Yi et al. The removal of antibiotic resistant bacteria and genes and inhibition of the horizontal gene transfer by contrastive research on sulfidated nanoscale zerovalent iron activating peroxymonosulfate or peroxydisulfate . | JOURNAL OF HAZARDOUS MATERIALS , 2022 , 423 . |
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摘要 ：
Using inhibitors to selectively suppress the activity of nitrite-oxidizing bacteria (NOB) was an emerging way to rapidly achieve partial nitrification (PN). This study explored the feasibility of inactivating NOB by a novel inhibitor chloroxylenol (PCMX) in real domestic wastewater. Different frequencies (periodic strategy and concentrative time strategy) of PCMX side-stream sludge treatment were used to achieve and maintain PN during 250 days. PN was realized by PCMX treatment once a day about 20 days, due to the inhibition of Nitrospira. PN was completely destroyed after 212 days by periodic strategy, caused by the increase of Candidatus Nitrotoga. PN maintained without PCMX in following 201 days by concentrative time strategy. The risks of PCMX were assessed and almost no PCMX was detected in the effluent of mainstream sequencing batch reactors. These results meant PN realized by PCMX side-stream sludge treatment was feasible and concentrative time strategy was a better operating strategy.
关键词 ：
Chloroxylenol Chloroxylenol Domestic wastewater Domestic wastewater Nitrite oxidizing bacteria Nitrite oxidizing bacteria Partial nitrification Partial nitrification Side-stream sludge treatment Side-stream sludge treatment
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| GB/T 7714 | Wang, Zhiqi , Gao, Jingfeng , Zhang, Da et al. Achieving stable and long-term partial nitrification of domestic wastewater by side-stream sludge treatment using a novel nitrite oxidation inhibitor chloroxylenol [J]. | BIORESOURCE TECHNOLOGY , 2021 , 342 . |
| MLA | Wang, Zhiqi et al. "Achieving stable and long-term partial nitrification of domestic wastewater by side-stream sludge treatment using a novel nitrite oxidation inhibitor chloroxylenol" . | BIORESOURCE TECHNOLOGY 342 (2021) . |
| APA | Wang, Zhiqi , Gao, Jingfeng , Zhang, Da , Dai, Huihui , Zhao, Yifan , Li, Dingchang et al. Achieving stable and long-term partial nitrification of domestic wastewater by side-stream sludge treatment using a novel nitrite oxidation inhibitor chloroxylenol . | BIORESOURCE TECHNOLOGY , 2021 , 342 . |
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摘要 ：
The addition of second metal (Co) to nanoscale iron particles (NIPs) is an attractive strategy to improve catalytic capacity. However, the nanoparticles tend to form chain-like aggregates. In this study, bacterial 16S rRNA gene, antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) from secondary effluent were significantly removed by Ginkgo biloba L. modified nanoscale iron-cobalt particles (GNICPs). When the Co loading, initial pH value, DO and dosage were 10%, 7.33, 8.94 mg/L and 1.12 g/L, some ARGs and MGEs could be reduced below the detection limit at the 2nd or 3rd cycle. Illumina MiSeq sequencing demonstrated that negative correlations were found between ARGs and reaction time/cycles. The predicted microbial functions by FAPROTAX database indicated GNICPs were effective in eliminating human_pathogens_all. Furthermore, oligotyping revealed all ARGs and MGEs were positively correlated with oligotype 10, which indicated GNICPs removed oligotype 10 easily.
关键词 ：
Antibiotic resistance genes Antibiotic resistance genes Function prediction Function prediction Ginkgo biloba L. modified nanoscale iron-cobalt particles Ginkgo biloba L. modified nanoscale iron-cobalt particles Oligotyping analysis Oligotyping analysis Secondary effluent Secondary effluent
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| GB/T 7714 | Duan Wanjun , Gao Jingfeng , Wu Zhilong et al. Enhanced removal of antibiotic resistance genes by nanoscale iron-cobalt particles modified with Ginkgo biloba L. leaf: Combining Illumina MiSeq sequencing and oligotyping analysis. [J]. | Bioresource technology , 2021 , 321 : 124453 . |
| MLA | Duan Wanjun et al. "Enhanced removal of antibiotic resistance genes by nanoscale iron-cobalt particles modified with Ginkgo biloba L. leaf: Combining Illumina MiSeq sequencing and oligotyping analysis." . | Bioresource technology 321 (2021) : 124453 . |
| APA | Duan Wanjun , Gao Jingfeng , Wu Zhilong , Dai Huihui , Wang Zhiqi , Li Dingchang et al. Enhanced removal of antibiotic resistance genes by nanoscale iron-cobalt particles modified with Ginkgo biloba L. leaf: Combining Illumina MiSeq sequencing and oligotyping analysis. . | Bioresource technology , 2021 , 321 , 124453 . |
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摘要 ：
Exposure to airborne particulate matter (PM2.5) is associated with cardiovascular diseases. In order to investigate the molecular mechanisms of air pollution-induced CVDs toxicity, human umbilical vein endothelial cells (HUVECs) were exposed to PM2.5 collected from January, 2016 winter in Beijing, China. We performed RNA sequencing to elucidate key molecular mechanism of PM 2.5-mediated toxicity in HUVECs. A total of 1753 genes, 864 up-regulated and 889 down-regulated, were observed to be differentially expressed genes (DEGs). Among these, genes involved in metabolic response, oxidative stress, inflammatory response, and vascular dysfunction were significantly differentially expressed (log2 FC > 4). The results were validated by quantitative real-time PCR (qPCR) and Western blot for CYP1B1, HMOX1, IL8, and GJA4. Pathway analysis revealed that DEGs were involved in the biological processes related to metabolism, inflammation, and host defense against environmental insults. This research is providing a further understanding of the mechanisms underlying PM2.5-induced cardiovascular diseases (CVDs).
关键词 ：
5 5 CVDs CVDs HUVEC HUVEC PM2 PM2 RNA-seq RNA-seq
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| GB/T 7714 | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis [J]. | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 . |
| MLA | Zhou, Zhixiang et al. "Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis" . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH (2021) . |
| APA | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara , Li, Wenke , Niu, Bingyu , Li, Jiangshuai et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 . |
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摘要 ：
In present study, copper (Cu), zinc (Zn), tetracycline (TC) and ampicillin (AMP) were selected to study the individual and synergistic effects of antibiotics and heavy metals on the microbial communities and resistance genes on polyvinyl chloride microplastics (PVC MPs) and surrounding sewage after 28 and 84 days. The results indicated that PVC MPs enriched many microorganisms from surrounding sewage, especially pathogenic bacteria such as Mycobacterium and Aquabacterium. The resistance gene with the highest abundance enriched on PVC MPs was tnpA (average abundance of 1.0 × 107 copies/mL sewage). The single presence of Zn, TC and AMP inhibited these enrichments for a short period of time (28 days). But the single presence of Cu and the co-existence of antibiotics and heavy metals inhibited these enrichments for a long period of time (84 days), resulting in relatively low microbial diversities and resistance genes abundances. Transpose tnpA had significantly positive correlations (p © 2020 Elsevier B.V.
关键词 ：
Antibiotics Antibiotics Bacteria Bacteria Chlorine compounds Chlorine compounds Genes Genes Heavy metals Heavy metals Microplastic Microplastic Polyvinyl chlorides Polyvinyl chlorides Sewage Sewage
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| GB/T 7714 | Zhao, Yifan , Gao, Jingfeng , Wang, Zhiqi et al. Responses of bacterial communities and resistance genes on microplastics to antibiotics and heavy metals in sewage environment [J]. | Journal of Hazardous Materials , 2021 , 402 . |
| MLA | Zhao, Yifan et al. "Responses of bacterial communities and resistance genes on microplastics to antibiotics and heavy metals in sewage environment" . | Journal of Hazardous Materials 402 (2021) . |
| APA | Zhao, Yifan , Gao, Jingfeng , Wang, Zhiqi , Dai, Huihui , Wang, Yuwei . Responses of bacterial communities and resistance genes on microplastics to antibiotics and heavy metals in sewage environment . | Journal of Hazardous Materials , 2021 , 402 . |
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摘要 ：
The biotransformation of triclosan (TCS) during wastewater treatment occurred frequently, while little researches are known the identity of microorganisms involved in the biodegradation process. In this work, DNA-based stable isotope probing (DNA-SIP) was occupied to investigate the TCS assimilation microbes originated from a full-scale cyclic activated sludge system in Beijing. Results of TCS removal pathway showed that the TCS removal in nitrification process was mainly contributed by the metabolism of heterotrophic bacteria, accounting for about 18.54%. DNA-SIP assay indicated that Sphingobium dominated the degradation of TCS. Oligotyping analysis further indicated that oligotype GCTAAT and ATGTTA of Sphingobium played important roles in degrading TCS. Furthermore, the Kyoto Encyclopedia of Genes and Genomes functional abundance statistics based on PICRUSt2 showed that glutathione transferase was the most prevalent enzyme involved in TCS metabolism, and TCS might be removed through microbial carbon metabolism. Metagenomics made clear that Sphingobium might play irrelevant role on the propagation of antibiotics resistance genes (ARGs), even though, it could degrade TCS. Thauera and Dechloromonas were identified as the key hosts of most ARGs. This study revealed the potential metabolic pathway and microbial ecology of TCS biodegradation in nitrification process of wastewater treatment system. © 2020 Elsevier B.V.
关键词 ：
Activated sludge process Activated sludge process Bacteria Bacteria Biodegradation Biodegradation DNA DNA Ecology Ecology Genes Genes Isotopes Isotopes Metabolism Metabolism Nitrification Nitrification Reclamation Reclamation Wastewater treatment Wastewater treatment
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| GB/T 7714 | Dai, Huihui , Gao, Jingfeng , Li, Dingchang et al. Metagenomics combined with DNA-based stable isotope probing provide comprehensive insights of active triclosan-degrading bacteria in wastewater treatment [J]. | Journal of Hazardous Materials , 2021 , 404 . |
| MLA | Dai, Huihui et al. "Metagenomics combined with DNA-based stable isotope probing provide comprehensive insights of active triclosan-degrading bacteria in wastewater treatment" . | Journal of Hazardous Materials 404 (2021) . |
| APA | Dai, Huihui , Gao, Jingfeng , Li, Dingchang , Wang, Zhiqi , Duan, Wanjun . Metagenomics combined with DNA-based stable isotope probing provide comprehensive insights of active triclosan-degrading bacteria in wastewater treatment . | Journal of Hazardous Materials , 2021 , 404 . |
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摘要 ：
Microplastics (MPs) and pharmaceuticals are common emerging pollutants in sewage, and their coexistence may have more negative effects on the environments. This study chose tetracycline (TC), ampicillin (AMP) and tridosan (TCS) to investigate the responses of antibiotic resistance genes (ARGs) and microbial communities on different MPs (polyvinyl chloride (PVC), polyethylene (PE)) biofilms (plastisphere). The adsorption capacity of three pharmaceuticals on PVC and PE decreased in the order of AMP > IC > TCS. PE was more conducive to microbial attachment than PVC. MPs led to the increase of the total copies of ARGs and mobile genetic elements (MGEs) in the sewage. Importantly, multidrug ARGs and MGEs were enriched on plastisphere. Furthermore, the co-occurrence of IC and MPs led to higher risks of spreading ARGs and MGEs. In addition, potential pathogenic bacteria Legionella, Mycobacterium, Neisseria and Arcobacter were more abundant on plastisphere than those in sewage, and these bacteria might be the hosts for ARGs and MGEs. This study showed that plastisphere could be repositories of ARGs and MGEs in sewage and accumulated potential pathogenic bacteria. (C) 2021 Elsevier B.V. All rights reserved.
关键词 ：
Adsorption Adsorption Antibiotic resistance genes Antibiotic resistance genes Microbial communities Microbial communities Pharmaceuticals Pharmaceuticals Plastisphere Plastisphere Potential pathogenic bacteria Potential pathogenic bacteria
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| GB/T 7714 | Wang, Zhiqi , Gao, Jingfeng , Zhao, Yifan et al. Plastisphere enrich antibiotic resistance genes and potential pathogenic bacteria in sewage with pharmaceuticals [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 768 . |
| MLA | Wang, Zhiqi et al. "Plastisphere enrich antibiotic resistance genes and potential pathogenic bacteria in sewage with pharmaceuticals" . | SCIENCE OF THE TOTAL ENVIRONMENT 768 (2021) . |
| APA | Wang, Zhiqi , Gao, Jingfeng , Zhao, Yifan , Dai, Huihui , Jia, Jingxin , Zhang, Da . Plastisphere enrich antibiotic resistance genes and potential pathogenic bacteria in sewage with pharmaceuticals . | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 768 . |
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摘要 ：
Antibiotic resistance genes (ARGs) are often detected in secondary effluents of wastewater treatment plants. Nanoscale zero valent iron (nZVI) modified by Ginkgo biloba L. leaf extract (G-nZVI) as an effective catalyst exhibited excellent activation of sodium persulfate (PS), which could achieve higher removal efficiency of ARGs than PS + nZVI system. However, the roles and specific components of Ginkgo biloba L. leaf (Ginkgo biloba L.) for nZVI modification are still not understood. X-ray diffraction and X-ray photoelectron spectroscopy of nZVI and G-nZVI indicated content of surface Fe2+ of G-nZVI increased by 1.66 times. Under such circumstances, removal efficiencies of genes were further compared by two systems (G + nZVI + PS/G-nZVI + PS). However, the degradation efficiencies of sul1, intI1 and bacterial 16S rRNA gene did not significantly differ. Therefore, the role of Ginkgo biloba L. extract was probably to increase active sites of nZVI by electron transfer, but not to prevent agglomeration of nZVI. Additionally, the findings of electron spin resonance elucidated that PS could be activated by Ginkgo biloba L. extract to produce radicals. Notably, metabolic interactive pathways (iPath) showed that there were accumulated lipids in nZVI, which may arise electron transfer and redox reactions. Additionally, the seven significantly down regulation metabolites might reduce the Fe3+ to Fe2+ and became the most important contributors to modify nZVI. © 2020 Elsevier B.V.
关键词 ：
Antibiotics Antibiotics Degradation Degradation Efficiency Efficiency Effluents Effluents Electron spin resonance spectroscopy Electron spin resonance spectroscopy Electron transitions Electron transitions Electron transport properties Electron transport properties Free radical reactions Free radical reactions Genes Genes Iron compounds Iron compounds Iron metallography Iron metallography Magnetic moments Magnetic moments Metabolites Metabolites Nanotechnology Nanotechnology Redox reactions Redox reactions RNA RNA Sewage treatment plants Sewage treatment plants Sodium compounds Sodium compounds Wastewater treatment Wastewater treatment X ray photoelectron spectroscopy X ray photoelectron spectroscopy
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| GB/T 7714 | Duan, Wanjun , Gao, Jingfeng , Li, Dingchang et al. Unravelling the roles of Ginkgo biloba L. for modification of nanoscale zero valent iron in persulfate system to remove antibiotic resistance genes by the tool of metabonomic analysis [J]. | Chemical Engineering Journal , 2021 , 417 . |
| MLA | Duan, Wanjun et al. "Unravelling the roles of Ginkgo biloba L. for modification of nanoscale zero valent iron in persulfate system to remove antibiotic resistance genes by the tool of metabonomic analysis" . | Chemical Engineering Journal 417 (2021) . |
| APA | Duan, Wanjun , Gao, Jingfeng , Li, Dingchang , Dai, Huihui , Wang, Zhiqi , Zhang, Wenzhi et al. Unravelling the roles of Ginkgo biloba L. for modification of nanoscale zero valent iron in persulfate system to remove antibiotic resistance genes by the tool of metabonomic analysis . | Chemical Engineering Journal , 2021 , 417 . |
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