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摘要 ：
针对日益提升的污水处理氮排放标准使低碳氮比污水的深度脱氮遇到严峻考验的问题,以实际生活污水为处理对象,通过长期的实验研究硫自养反硝化滤池(简称硫池)脱氮性能和对TN去除的贡献。结果表明,当硫池的水力停留时间不低于1.4 h时,NO_x~--N的去除率能达到100%,硫池的NO_x~--N最高去除负荷为0.25 kg/(m~3·d)。硫池的硫酸根生成量和硝酸氮去除量的质量浓度比(Δρ(SO_4~(2-))/Δρ(NO_3~--N))为6.50,低于理论的7.54,表明硫池还存在着部分异养反硝化或异养硫酸盐还原作用,或硫的不完全氧化。可为低碳氮比实际污水的深度脱氮提供现实依据。
关键词 ：
硫自养反硝化 硫自养反硝化 去除负荷 去除负荷 深度脱氮 深度脱氮 贡献率 贡献率 硫酸盐 硫酸盐
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| GB/T 7714 | 徐康康 , 闫慧娟 , 崔有为 . A~2/O硫自养反硝化处理低碳氮比废水计量学研究 [J]. | 水处理技术 , 2021 , 47 (07) : 118-122 . |
| MLA | 徐康康 等. "A~2/O硫自养反硝化处理低碳氮比废水计量学研究" . | 水处理技术 47 . 07 (2021) : 118-122 . |
| APA | 徐康康 , 闫慧娟 , 崔有为 . A~2/O硫自养反硝化处理低碳氮比废水计量学研究 . | 水处理技术 , 2021 , 47 (07) , 118-122 . |
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摘要 ：
Efficient start-up, cultivation and maintenance of aerobic granular sludge (AGS) in continuous flow reactors (CFRs) is critical for its full-scale application in wastewater treatment plants (WWTPs). The successful implementation of AGS under continuous flow condition depends on the configuration of CFR that provides the selective pressures and hydraulic shear force (HSF) to sludge required for rapid granulation. Important progress has been witnessed with respect to CFR configurations in recent decades for in-situ granulation of aerobic sludge. This review summarized the difficulties of granulation during continuous flow, as well as the reactor configuration, granulation mechanism and the AGS characteristics, along with the future research prospects. It is of significance to promote the innovation of continuous flow configuration in order to realize the AGS application in mainstream WWTPs in the future. (c) 2021 Society of Chemical Industry (SCI).
关键词 ：
granulation mechanism granulation mechanism granulation in continuous flow granulation in continuous flow selective pressure selective pressure reactor configuration reactor configuration aerobic granular sludge (AGS) aerobic granular sludge (AGS)
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| GB/T 7714 | Yan, Jin-Lei , Cui, You-Wei , Huang, Ji-Lin . Continuous flow reactors for cultivating aerobic granular sludge: configuration innovation, principle and research prospect [J]. | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY , 2021 , 96 (10) : 2721-2734 . |
| MLA | Yan, Jin-Lei 等. "Continuous flow reactors for cultivating aerobic granular sludge: configuration innovation, principle and research prospect" . | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 96 . 10 (2021) : 2721-2734 . |
| APA | Yan, Jin-Lei , Cui, You-Wei , Huang, Ji-Lin . Continuous flow reactors for cultivating aerobic granular sludge: configuration innovation, principle and research prospect . | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY , 2021 , 96 (10) , 2721-2734 . |
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摘要 ：
本发明公开了一种快速富集反硝化硫氧化菌群的方法。该技术按两段式磁强化模式进行：第一阶段将清洗后的新鲜活性污泥接种至磁诱导反应器中，加入反硝化硫氧化菌群生长所需的富集底物溶液进行搅拌混合培养并控制磁场为5‑70mT，待底物利用完全后停止搅拌，静置沉淀后排出上清液，重复上述过程2‑3次；第二阶段调整磁场强度为5‑20mT重复上述培养过程2‑5次，直至所培养菌群实现快速脱氮并保持脱氮性能稳定。该方法通过磁场诱导产生磁生物效应，激发混合菌群中反硝化硫氧化菌群的快速生长和代谢活性，实现反硝化硫氧化菌群的快速富集。
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| GB/T 7714 | 崔有为 , 闫慧娟 . 一种快速富集反硝化硫氧化菌群的方法 : CN202110071863.8[P]. | 2021-01-20 . |
| MLA | 崔有为 等. "一种快速富集反硝化硫氧化菌群的方法" : CN202110071863.8. | 2021-01-20 . |
| APA | 崔有为 , 闫慧娟 . 一种快速富集反硝化硫氧化菌群的方法 : CN202110071863.8. | 2021-01-20 . |
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摘要 ：
以赤泥为原料通过共沉淀法制备了双层金属氢氧化物(LDHs),并用于废水中磷酸盐的吸附。结果表明,LDHs吸附磷酸盐符合Langmuir等温吸附和准2级动力学模型,吸附反应为单层表面化学吸附,自发吸热反应。LDHs对磷酸盐的吸附容量与温度和初始磷酸盐含量呈正相关,与溶液的pH呈负相关,实际P的最大吸附容量为88.24 mg/g。废水中共存的F-对磷酸盐的吸附有显著的抑制作用。LDHs吸附磷酸盐可能主要通过配位作用、离子交换和沉淀反应协同作用实现的。研究可为赤泥作为环境污染治理材料的开发提供一种可能的利用方式。
关键词 ：
吸附 吸附 层状双金属氢氧化物(LDHs) 层状双金属氢氧化物(LDHs) 磷酸盐 磷酸盐 赤泥 赤泥
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| GB/T 7714 | 李延龙 , 崔有为 . 赤泥基LDHs制备及其吸附废水中磷酸盐特性 [J]. | 水处理技术 , 2021 , 47 (11) : 36-41 . |
| MLA | 李延龙 等. "赤泥基LDHs制备及其吸附废水中磷酸盐特性" . | 水处理技术 47 . 11 (2021) : 36-41 . |
| APA | 李延龙 , 崔有为 . 赤泥基LDHs制备及其吸附废水中磷酸盐特性 . | 水处理技术 , 2021 , 47 (11) , 36-41 . |
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摘要 ：
The utilization of halophilic bioresources is limited due to a lack of isolation and characterization work. A halophilic bacterium strain SND-01 of Exiguobacterium mexicanum was isolated in this study, which is the first report on its novel function in heterotrophic nitrification-aerobic denitrification (HN-AD). The strain SND-01 is slightly halophilic, surviving at 0 up to 9% (w/v) salinity. When utilizing ammonium, nitrate or nitrite as the sole nitrogen source in aerobic conditions, the isolated strain showed the maximum nitrogen removal rate of 2.24 ± 0.14 mg/(L·h), 3.63 ± 0.21 mg/(L·h) and 2.30 ± 0.23 mg/(L·h), respectively. Functional genes and key enzymes involved in heterotrophic-aerobic nitrogen transformations were characterized, establishing the pathway of HN-AD. The nitrogen removal via HN-AD is dependent on the C/N ratio, salinity and temperature. The halophilic Exiguobacterium mexicanum strain SND-01 shows a significant potential in biotreatment of saline wastewater in an easy and cost-effective way.
关键词 ：
Aerobic denitrification Aerobic denitrification Halophilic bacterium Halophilic bacterium Heterotrophic nitrification Heterotrophic nitrification Nitrogen removal Nitrogen removal Saline wastewater Saline wastewater
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| GB/T 7714 | Cui Yuan , Cui You-Wei , Huang Ji-Lin . A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification. [J]. | Bioresource technology , 2021 , 333 : 125189 . |
| MLA | Cui Yuan 等. "A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification." . | Bioresource technology 333 (2021) : 125189 . |
| APA | Cui Yuan , Cui You-Wei , Huang Ji-Lin . A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification. . | Bioresource technology , 2021 , 333 , 125189 . |
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摘要 ：
The reuse of zirconium-modified red mud (Zr-RM) as a phosphate adsorbent for wastewater treatment was assessed herein. The physicochemical properties and phosphate adsorption performance of Zr-RM were investigated, revealing a single-layer adsorption pattern according to the Langmuir isotherm model. The adsorption rate followed a pseudo-second-order model. The maximum phosphate adsorption capacity was 33.14 mg/g and was positively correlated with temperature and initial phosphate concentration, and negatively correlated with the solution pH. In a solution containing fluoride and sulfate ions, due to competitive adsorption, the adsorption capacity of phosphate decreased. The material and wastewater treatment costs of Zr-RM are 0.99 and 61.3 $/ kg of P, respectively. This study showed a potential method for using RM with high value for environmental protection.
关键词 ：
Adsorption Adsorption Phosphate removal Phosphate removal Red mud Red mud Waste reuse Waste reuse Zirconium modification Zirconium modification
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| GB/T 7714 | Li, Yan-Long , Alam, Fakhri , Cui, You-Wei . Red mud reuse for phosphate adsorption via zirconium modification: performance, kinetics, and mechanism [J]. | DESALINATION AND WATER TREATMENT , 2021 , 225 : 331-339 . |
| MLA | Li, Yan-Long 等. "Red mud reuse for phosphate adsorption via zirconium modification: performance, kinetics, and mechanism" . | DESALINATION AND WATER TREATMENT 225 (2021) : 331-339 . |
| APA | Li, Yan-Long , Alam, Fakhri , Cui, You-Wei . Red mud reuse for phosphate adsorption via zirconium modification: performance, kinetics, and mechanism . | DESALINATION AND WATER TREATMENT , 2021 , 225 , 331-339 . |
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摘要 ：
Suitability of different substrates for enriched mixed microbial cultures (MMCs) is of importance to the polyhydroxyalkanoate (PHA) fermentation using renewable carbon. In this study, three enriched MMCs were evaluated for their fermentation features and kinetics with different carbon sources (sodium acetate, glucose, or starch). The results showed that the highly specific bacterial community composition was developed depending on the applied carbon source. Correspondence analysis suggested that the genus affiliated in Gammaproteobacteria_unclassified was related to 3-hydroxybutyrate (HB) synthesis in acetate-fed MMC (relative abundance of 38%) and glucose-fed MMC (relative abundance of 76.7%), whereas Vibrio genus was related to 3-hydroxyvalerate (HV) production in glucose-fed MMC (relative abundance of 0.4%) and starch-fed MMC (relative abundance of 94.6%). The acetate-fed MMC could not use glucose and starch as fermentation carbon sources, showing the limitation of microbial species developed with the specific metabolic substrate. Glucose-fed MMC produced the highest PHA cell content of 64.2% cell dry weight when using sodium acetate as the fermentation carbon. Glucose-fed MMC showed wide resilience and adaptation to various carbon sources. When actual landfill leachate was used for fermentation by glucose-fed MMC, maximum PHA cell content of 45.5% cell dry weight and the PHA volumetric productivity of 0.265 g PHA/(L center dot h) were obtained. This study suggested carbon sources applied in the MMC enrichment stage had a significant influence on utilization of carbon in the fermentation stage.
关键词 ：
Carbon source adaptation Carbon source adaptation Fermentation Fermentation Landfill leachate Landfill leachate Mixed microbial culture (MMC) Mixed microbial culture (MMC) Polyhydroxyalkanoates (PHAs) Polyhydroxyalkanoates (PHAs)
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| GB/T 7714 | Zhao, Jin , Cui, You-Wei , Zhang, Hong-Yu et al. Carbon Source Applied in Enrichment Stage of Mixed Microbial Cultures Limits the Substrate Adaptability for PHA Fermentation Using the Renewable Carbon [J]. | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY , 2021 , 193 (10) : 3253-3270 . |
| MLA | Zhao, Jin et al. "Carbon Source Applied in Enrichment Stage of Mixed Microbial Cultures Limits the Substrate Adaptability for PHA Fermentation Using the Renewable Carbon" . | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 193 . 10 (2021) : 3253-3270 . |
| APA | Zhao, Jin , Cui, You-Wei , Zhang, Hong-Yu , Gao, Ze-Liang . Carbon Source Applied in Enrichment Stage of Mixed Microbial Cultures Limits the Substrate Adaptability for PHA Fermentation Using the Renewable Carbon . | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY , 2021 , 193 (10) , 3253-3270 . |
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摘要 ：
Granulation of halophilic activated sludge is an important solution to solve the problem of solid-liquid separation in biological treatment of saline wastewater. This study demonstrated that by adding divalent cations into the saline influent with low organic load, halophilic granular sludge with an average diameter of 910 ± 10 μm can be cultivated. The close correlation between divalent cations and particle size indicated that Ca2+ played a major role in the granulation process. Ca2+ was accumulated in halophilic granular sludge, which provided an inorganic carrier for microbial aggregation and leaded to the dominance of halophilic bacteria of the family Flavobacteriaceae. The halophilic bacteria secreted a large amount of extracellular polymeric substances (EPS), which contained 70.0 ± 0.02% protein. By enhancing the EPS network of protein and Ca2+, halophilic granular sludge was formed. The addition of Mg2+ enhanced the network of Mg2+ and loosely bound EPS, which could be destroyed due to Na+ substitution. This study provides an effective granulation method for halophilic activated sludge. © 2020 Elsevier Ltd
关键词 ：
Bacteria Bacteria Biological water treatment Biological water treatment Granular materials Granular materials Granulation Granulation Particle size Particle size Positive ions Positive ions Proteins Proteins Wastewater treatment Wastewater treatment
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| GB/T 7714 | Cui, You-Wei , Huang, Ji-Lin , Alam, Fakhri . Fast granulation of halophilic activated sludge treating low-strength organic saline wastewater via addition of divalent cations [J]. | Chemosphere , 2021 , 264 . |
| MLA | Cui, You-Wei et al. "Fast granulation of halophilic activated sludge treating low-strength organic saline wastewater via addition of divalent cations" . | Chemosphere 264 (2021) . |
| APA | Cui, You-Wei , Huang, Ji-Lin , Alam, Fakhri . Fast granulation of halophilic activated sludge treating low-strength organic saline wastewater via addition of divalent cations . | Chemosphere , 2021 , 264 . |
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摘要 ：
How polymer synthesis is mobilized or activated as a biological response of Haloferax mediterranei against hypertonic conditions remains largely unexplored. This study investigated the protein expression of H. mediterranei in response to high salinity by using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis. The microbes were harvested at end of fermentation at the NaCl salinity of 75 and 250 g L-1. Among the identified 2123 proteins, 170 proteins were differentially expressed. Gene ontology annotation revealed that the highest number of proteins was annotated in biological process category, which was responsible for metabolic process, cellular component and catalytic activity. Differentially expressed proteins were belonged to the class of response to stimulus as well as catalytic activity and binding. Under high salinity conditions, three pathways were established as key responses of PHA and EPS production to hypertonic pressure. Two overexpressed proteins, beta-ketoacyl-ACP reductase and 3-hydroxyacyl-CoA dehydrogenase, enhanced the synthesis of PHAs. The serine-pyruvate transaminase and serine-glyoxylate transaminase were upregulated, thereby increasing the conversion of glucose to PHA. Downregulated levels of sulfate-adenylyl transferase and adenylyl-sulfate kinase could cause diminished EPS synthesis. This study could contribute to better understanding of the proteomic mechanisms of the synthesized polymers in defending against salt stress. SIGNIFICANCE: Haloferax mediterranei, a family member of halophilic archaea, is well known for its fermentative production of poly-β-hydroxyalkanoates (PHAs). PHAs are natural polymers that exhibit great potential in a wide range of applications such as a good alternative to petroleum-based plastics and the biocompatible material. For decades, the functional role of PHAs synthesized by H. mediterranei is deemed to be carbon and energy reservations. The finding proved that differential production of PHA and EPS in H. mediterranei exposed to elevated salinity was caused by differential protein expression. This is the first report on how PHA and EPS synthesized by H. mediterranei is mobilized as the response of increased salinity, contributing to the understanding of halophilic archaea's response to hypertonic stress and the precise control of fermentation production. Despite its advantages as a PHA cell factory, H. mediterranei synthesized EPS simultaneously, thereby lowering the maximum yield of PHA production. Overall, salinity can be used as a vital microbial fermentation parameter to obtain the highest harvest of PHA, as well as the lowest EPS synthesis in industrial fermentation.
关键词 ：
Exopolysaccharide Exopolysaccharide Haloferax mediterranei Haloferax mediterranei Hypertonic condition Hypertonic condition iTRAQ analysis iTRAQ analysis Metabolism Metabolism Polyhydroxyalkanoates Polyhydroxyalkanoates
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| GB/T 7714 | Pacholak Amanda , Gao Ze-Liang , Gong Xiao-Yu et al. The metabolic pathways of polyhydroxyalkanoates and exopolysaccharides synthesized by Haloferax mediterranei in response to elevated salinity. [J]. | Journal of proteomics , 2021 , 232 : 104065 . |
| MLA | Pacholak Amanda et al. "The metabolic pathways of polyhydroxyalkanoates and exopolysaccharides synthesized by Haloferax mediterranei in response to elevated salinity." . | Journal of proteomics 232 (2021) : 104065 . |
| APA | Pacholak Amanda , Gao Ze-Liang , Gong Xiao-Yu , Kaczorek Ewa , Cui You-Wei . The metabolic pathways of polyhydroxyalkanoates and exopolysaccharides synthesized by Haloferax mediterranei in response to elevated salinity. . | Journal of proteomics , 2021 , 232 , 104065 . |
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摘要 ：
为了探究好氧丰盛/缺氧饥饿(O/A-F/F)模式驯化的微生物的内源积累能力和缺氧段内源反硝化的特性,并比较其与传统好氧丰盛/饥饿(O-F/F)驯化模式的差别,以添加乙酸的生活污水为底物,在F/F=0.1的条件下,采用O/A-F/F和O-F/F两种不同模式驯化活性污泥.结果表明,O/A-F/F模式的PHA产率为0.52,O-F/F模式的PHA产率为0.78.虽然O/A-F/F模式的PHA产率低,但是O/A-F/F模式具有内源反硝化功能,比内源反硝化速率为2.76 mg/(h·g).O/A-F/F模式下还原1 mol的硝酸盐需要2 mol的内源PHA.2种选择模式下的菌群结构呈现差异,O/A-F/F模式下优势菌群为Betaproteobacteria、Unclassified Bacteroidetes、Lgnavibacteria和Bacteroidia.O-F/F模式富集的微生物主要为Betaproteobacteria、Flavobacteria、Alphaproteobacteria和Gammaproteobacteria.O/A-F/F运行模式可以实现好氧阶段污水中有机物的内源储存并在缺氧段用于反硝化.
关键词 ：
好氧丰盛/饥饿 好氧丰盛/饥饿 菌群结构 菌群结构 糖原 糖原 内源反硝化 内源反硝化 好氧丰盛/缺氧饥饿 好氧丰盛/缺氧饥饿 聚羟基脂肪酸酯 聚羟基脂肪酸酯
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| GB/T 7714 | 崔有为 , 王好韩 , 金常林 . 好氧丰盛/缺氧饥饿模式驯化微生物积累内源聚合物并实现内源反硝化 [J]. | 北京工业大学学报 , 2020 , 46 (11) : 1283-1290 . |
| MLA | 崔有为 et al. "好氧丰盛/缺氧饥饿模式驯化微生物积累内源聚合物并实现内源反硝化" . | 北京工业大学学报 46 . 11 (2020) : 1283-1290 . |
| APA | 崔有为 , 王好韩 , 金常林 . 好氧丰盛/缺氧饥饿模式驯化微生物积累内源聚合物并实现内源反硝化 . | 北京工业大学学报 , 2020 , 46 (11) , 1283-1290 . |
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