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学者姓名：樊晓燕
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摘要 ：
Effect of copper (Cu) on antibiotic resistance genes (ARGs) and bacterial/archaeal community of activated sludge under gradient increasing (0.5-10 mg/L) or decreasing exposure (10-0.5 mg/L) modes was explored. Here, 29 genes were detected among 48 selected ARGs and mobile gene elements (MGEs). Two exposure modes showed dissimilar effects on ARGs and distribution was more affected by environmental concentrations of Cu, which promoted transmission of ARGs (multiple drug resistance and sulfonamide). Cellular protection was main resistance mechanism, which was less inhibited than efflux pumps. The tnpA-02, as main MGE, interacted closely with ARGs (sul2, floR, etc.). Gradient increasing exposure mode had more effects on bacterial/archaeal structure and composition. Bacteria were main hosts for specific ARGs and tnpA-02, while archaea carried multiple ARGs (cmx(A), adeA, etc.), and bacteria (24.24 %) contributed more to changes of ARGs than archaea (19.29 %). This study clarified the impacts of Cu on the proliferation and transmission of ARGs.
关键词 ：
archaeal communities archaeal communities decreasing exposure decreasing exposure Copper Copper Correlations Correlations Antibiotic resistance genes Antibiotic resistance genes Bacterial Bacterial modes modes Gradient increasing Gradient increasing
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| GB/T 7714 | Gao, Yu -Xi , Li, Xing , Fan, Xiao-Yan et al. Fates of antibiotic resistance genes and bacterial/archaeal communities of activated sludge under stress of copper: Gradient increasing/decreasing exposure modes [J]. | BIORESOURCE TECHNOLOGY , 2022 , 363 . |
| MLA | Gao, Yu -Xi et al. "Fates of antibiotic resistance genes and bacterial/archaeal communities of activated sludge under stress of copper: Gradient increasing/decreasing exposure modes" . | BIORESOURCE TECHNOLOGY 363 (2022) . |
| APA | Gao, Yu -Xi , Li, Xing , Fan, Xiao-Yan , Zhao, Jun-Ru , Zhang, Zhong-Xing . Fates of antibiotic resistance genes and bacterial/archaeal communities of activated sludge under stress of copper: Gradient increasing/decreasing exposure modes . | BIORESOURCE TECHNOLOGY , 2022 , 363 . |
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摘要 ：
For comprehensive insights into the effects of disinfection strategy on antibiotic resistance genes (ARGs) in pipe wall biofilm to ensure the biosafety of domestic hot water system (DHWS), this study investigated the occurrence, removal and interactions of ARGs, as well as their potential hosts. The shifts in biofilm community composition and microbial functions (level three) were observed under different disinfection strategies. A total of 40 ARG subtypes within seven antibiotic types were predicted. The total gene counts ranged from 6.10 x 104 to 2.31 x 105, and decreased after the chlorine, chlorine dioxide, silver ions (Ag+), ultraviolet (UV), and Ag+-UV disinfection. The ARGs in samples under different disinfection strategies were divided into four groups, and the removal and enrichment of the same ARGs in each group were almost consistent. The complex interactions among ARGs were weakened and altered by disinfection (edges decreased from 237 to 178), with yebQ and marC as the hub ARGs, but positive correlations were still dominant. Moreover, 36 nonpathogenic bacteria, including high- (Blastocatella), mid- (Ornatilinea) and low-abundant genera (Bdellovibrio), as well as 18 potential pathogens (Vibrio and Bacillus) were identified as the potential hosts harboring ARGs. Overall, this study provides some new insights into the occurrence of ARGs and their fate under eight disinfection strategies, which is of great significance for a comprehensive understanding and control of ARGs in DHWS.
关键词 ：
Antibiotic resistance genes Antibiotic resistance genes Disinfection Disinfection Domestic hot water system Domestic hot water system Potential hosts Potential hosts Removal and enrichment Removal and enrichment
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| GB/T 7714 | Li, Na , Li, Xing , Yang, Yan-Lin et al. Antibiotic resistance genes in pipe wall biofilm under eight disinfection strategies in domestic hot water system: Occurrence, removal and interactions [J]. | JOURNAL OF WATER PROCESS ENGINEERING , 2021 , 43 . |
| MLA | Li, Na et al. "Antibiotic resistance genes in pipe wall biofilm under eight disinfection strategies in domestic hot water system: Occurrence, removal and interactions" . | JOURNAL OF WATER PROCESS ENGINEERING 43 (2021) . |
| APA | Li, Na , Li, Xing , Yang, Yan-Lin , Fan, Xiao-Yan . Antibiotic resistance genes in pipe wall biofilm under eight disinfection strategies in domestic hot water system: Occurrence, removal and interactions . | JOURNAL OF WATER PROCESS ENGINEERING , 2021 , 43 . |
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摘要 ：
A pilot-scale two-stage tidal flow constructed wetland (TFCW) with working volume of 0.46 m3/d packing with shale ceramsite (SC) and activated alumina (AA) was constructed (named as SC-AA-TFCW) for nutrients removal at low temperature ( © 2020 Elsevier Ltd
关键词 ：
Activated alumina Activated alumina Aerobic bacteria Aerobic bacteria Aluminum oxide Aluminum oxide Ascorbic acid Ascorbic acid Denitrification Denitrification Dissolved oxygen Dissolved oxygen Nutrients Nutrients Phosphorus Phosphorus Temperature Temperature Wetlands Wetlands
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| GB/T 7714 | Tan, Xu , Yang, Yan-Ling , Liu, Yong-Wang et al. The synergy of porous substrates and functional genera for efficient nutrients removal at low temperature in a pilot-scale two-stage tidal flow constructed wetland [J]. | Bioresource Technology , 2021 , 319 . |
| MLA | Tan, Xu et al. "The synergy of porous substrates and functional genera for efficient nutrients removal at low temperature in a pilot-scale two-stage tidal flow constructed wetland" . | Bioresource Technology 319 (2021) . |
| APA | Tan, Xu , Yang, Yan-Ling , Liu, Yong-Wang , Yin, Wen-Chao , Fan, Xiao-Yan . The synergy of porous substrates and functional genera for efficient nutrients removal at low temperature in a pilot-scale two-stage tidal flow constructed wetland . | Bioresource Technology , 2021 , 319 . |
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摘要 ：
This study aimed to explore the dynamics of microbial communities and antibiotic resistance genes (ARGs) during biofilm formation on polypropylene random (PPR), polyvinyl chloride and stainless steel pipes in domestic hot water system (DHWS), as well as their interactions. Full-scale classification was used to divide abundant and rare genera with 0.1% and 1% as the thresholds. The biofilm community structure presented a temporal pattern, which was mainly determined by conditionally rare or abundant taxa (CRAT) and conditionally rare taxa (CRT). The dynamics of microbial community during biofilm formation were observed, and the effect of pipe material on conditionally abundant taxa (CAT) and CRAT was greater than CRT and rare taxa (RT). CRAT showed the most complex internal associations and were identified as the core taxa. Notably, CRT and RT with low relative abundance, also played an important role in the network. For potential pathogens, 17 genera were identified in this study, and their total relative abundance was the highest (3.6-28.9%) in PPR samples. Enterococcus of CRAT was the dominant potential pathogen in young biofilms. There were 36 more co-exclusion patterns (140) observed between potential pathogens and nonpathogenic bacteria than co-occurrence (104). A total of 38 ARGs were predicted, and 109 negative and 165 positive correlations were detected between them. Some potential pathogens (Escherichia/Shigella and Burkholderia) and nonpathogenic bacteria (Meiothermus and Sphingopyxis) were identified as the possible hosts of ARGs. This study is helpful for a comprehensive understanding of the biofilm microbial community and ARGs, and provides a reference for the management and biosafety guarantee of newly-built DHWS.
关键词 ：
Antibiotic resistance genes Antibiotic resistance genes Full-scale classification Full-scale classification Network analysis Network analysis Pipe materials Pipe materials Pipe wall biofilms Pipe wall biofilms Potential pathogens Potential pathogens
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| GB/T 7714 | Li Na , Li Xing , Zhang Hui-Jin et al. Microbial community and antibiotic resistance genes of biofilm on pipes and their interactions in domestic hot water system. [J]. | The Science of the total environment , 2021 , 767 : 144364 . |
| MLA | Li Na et al. "Microbial community and antibiotic resistance genes of biofilm on pipes and their interactions in domestic hot water system." . | The Science of the total environment 767 (2021) : 144364 . |
| APA | Li Na , Li Xing , Zhang Hui-Jin , Fan Xiao-Yan , Liu Yuan-Kun . Microbial community and antibiotic resistance genes of biofilm on pipes and their interactions in domestic hot water system. . | The Science of the total environment , 2021 , 767 , 144364 . |
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摘要 ：
Silver-loaded stainless steel (Ag-SS) was applied in domestic hot water system (DHWS) to control the biofilm contamination. The antibacterial properties of Ag-SS, as well as the bacterial communities, potential pathogens and antibiotic resistance genes (ARGs) of pipe wall biofilm were investigated, with SS as control. Ag-SS main-tained a high antibacterial efficiency on heterotrophic bacteria (70.60 ? 13.22 %) and Escherichia coli (73.56 ? 10.62 %) by contact bacteriostasis. Dominant phyla exhibited a lower relative abundance in Ag-SS than SS. The bacteria were further classified into abundant (? 1 %), moderately abundant (0.1-1 %) and rare (? 0.1 %) taxa to investigate their responses to silver. There were 15.33 % of shared operational taxonomic units (OTUs) belonged to abundant taxa, while most unique OTUs (> 80 %) of each sample were identified as rare taxa. As the primary abundant genera, Mesorhizobium and Bradyrhizobium exhibited resistant and sensitive to silver, respectively. Most dominant moderately abundant and rare genera were resistant to silver. Moreover, among 19 detected potential pathogens, Legionella, Parachlamydia and Aeromonas were sensitive to silver, while Pseudo-monas and Acinetobacter were resistant to silver. Furthermore, a total of 48 ARGs were detected and they exhibited similar distributions in both samples. The total abundance of ARGs in Ag-SS increased by 0.08 fold compared with that in SS, with efflux pumps as the main resistance mechanisms. This study highlights the different responses of abundant, moderately abundant and rare taxa to silver, and the occurrences of ARGs, confirming that Ag-SS was an effective disinfection strategy for biosafety protection of DHWS.
关键词 ：
Abundant and rare taxa Abundant and rare taxa Antibacterial efficiency Antibacterial efficiency Antibiotic resistance genes Antibiotic resistance genes Pipe wall biofilm Pipe wall biofilm Silver-loaded stainless steel Silver-loaded stainless steel
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| GB/T 7714 | Li, Na , Li, Xing , Shi, Zhi-Yuan et al. Bacterial communities, potential pathogens and antibiotic resistance genes of silver-loaded stainless steel pipe wall biofilm in domestic hot water system [J]. | JOURNAL OF WATER PROCESS ENGINEERING , 2021 , 40 . |
| MLA | Li, Na et al. "Bacterial communities, potential pathogens and antibiotic resistance genes of silver-loaded stainless steel pipe wall biofilm in domestic hot water system" . | JOURNAL OF WATER PROCESS ENGINEERING 40 (2021) . |
| APA | Li, Na , Li, Xing , Shi, Zhi-Yuan , Fan, Xiao-Yan , Zhou, Zhi-Wei . Bacterial communities, potential pathogens and antibiotic resistance genes of silver-loaded stainless steel pipe wall biofilm in domestic hot water system . | JOURNAL OF WATER PROCESS ENGINEERING , 2021 , 40 . |
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摘要 ：
Constructed wetlands (CWs) usually exhibit limits in functional redundancy and diversity of microbial community contributing to lower performances of nutrients removal in decentralized domestic sewage treatment. To address this quandary, heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria was added in tidal flow CWs (TFCWs) developing for nitrogen (N) and phosphorus (P) removal. With addition of HN-AD bacteria, TFCWs could be setup more rapidly and obtained better removal efficiencies of 66.9%-70.1% total nitrogen (TN), and 88.2%-92.4% total phosphorus (TP) comparing with control systems (TN: 53.9%; TP: 83.9%) during stable operation. Typical-cycles variations showed that TFCWs with addition of HN-AD bacteria promoted NO3--N and NH4+-N removal respectively under hydraulic retention time (HRT) of 14 h and 8 h with slight NO2--N accumulation. Activated alumina (AA) coupled with HN-AD bacteria decreased P release and relieved its poor removal performance in CWs. Based on metagenomic taxa and functional annotation, Pseudomonas and Thauera played pivotal roles in N removal in TFCWs. Furthermore, gradient oxic environments by 8 h-HRT promoted co-occurrence of heterotrophic nitrifiers (mostly Pseudomonas stutzeri) and autotrophic nitrifiers (mostly Nitrosomonas europaea. and Nitrospira sp.) which potentially accelerated NH4+-N transformation by elevated nitrification and denitrification related genes (e.g. amoABC, hao, napA and nirS genes). Meanwhile, the addition of HN-AD bacteria stimulated nirA and gltD genes of N assimilation processes probably leading to NH4+-N directly removal. The conceptual model of multi-metabolism regulation by HN-AD process highlighted importance of glk, gap2 and PK genes in glycolysis pathway which were vital drivers to nutrients metabolism. Overall, this study provides insights into how ongoing HN-AD bacteria addition effected microbial consortia and metabolic pathways, serving theoretical basis for its engineered applications of TFCWs in decentralized domestic sewage treatment. (C) 2021 Elsevier B.V. All rights reserved.
关键词 ：
Tidal flow constructed wetlands Tidal flow constructed wetlands Heterotrophic nitrification-aerobic denitrification bacteria Heterotrophic nitrification-aerobic denitrification bacteria Metagenomics Metagenomics Enhanced N and P removal Enhanced N and P removal
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| GB/T 7714 | Tan, Xu , Yang, Yan-Ling , Li, Xing et al. Multi-metabolism regulation insights into nutrients removal performance with adding heterotrophic nitrification-aerobic denitrification bacteria in tidal flow constructed wetlands [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 796 . |
| MLA | Tan, Xu et al. "Multi-metabolism regulation insights into nutrients removal performance with adding heterotrophic nitrification-aerobic denitrification bacteria in tidal flow constructed wetlands" . | SCIENCE OF THE TOTAL ENVIRONMENT 796 (2021) . |
| APA | Tan, Xu , Yang, Yan-Ling , Li, Xing , Gao, Yu-Xi , Fan, Xiao-Yan . Multi-metabolism regulation insights into nutrients removal performance with adding heterotrophic nitrification-aerobic denitrification bacteria in tidal flow constructed wetlands . | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 796 . |
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摘要 ：
The responses of microbial communities and antibiotic resistance genes (ARGs) to azithromycin and copper combined pollution under gradient increasing (from 0.5 to 10 mg/L) and decreasing exposure (from 10 to 0.5 mg/L) modes were investigated. Nitrification was inhibited more obviously under gradient increasing exposure mode. Responses of archaeal community and function structure were more obvious than bacteria under both exposure modes. The dominant bacterial and archaeal compositions (Hyphomicrobium, Euryarchaeota, etc.) were affected by two exposure modes, except some rare archaea (Methanoregula and Methanosarcina). There were more positive correlations between bacteria and archaea, and Nitrospira was keystone genus. Ammonia-oxidizing archaea (0.37-3.06%) and complete ammonia oxidizers (Nitrospira_ENR4) were enriched, and Nitrososphaera_viennensis was closely related to denitrifying genes (napA/B, nosZ, etc.). 50 ARG subtypes were detected and specific ARG subtypes (aac, ImrA, etc.) proliferated in two exposure modes. Bacteria and archaea were common hosts for 24 ARGs and contributed to their shifts.
关键词 ：
Combined pollution Combined pollution Potential hosts Potential hosts Gradient increasing and decreasing exposure modes Gradient increasing and decreasing exposure modes Bacterial and archaea communities Bacterial and archaea communities Antibiotic resistance genes Antibiotic resistance genes
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| GB/T 7714 | Gao, Yu-Xi , Li, Xing , Zhao, Jun-Ru et al. Impacts of combined pollution under gradient increasing and gradient decreasing exposure modes on activated sludge: Microbial communities and antibiotic resistance genes [J]. | BIORESOURCE TECHNOLOGY , 2021 , 345 . |
| MLA | Gao, Yu-Xi et al. "Impacts of combined pollution under gradient increasing and gradient decreasing exposure modes on activated sludge: Microbial communities and antibiotic resistance genes" . | BIORESOURCE TECHNOLOGY 345 (2021) . |
| APA | Gao, Yu-Xi , Li, Xing , Zhao, Jun-Ru , Zhang, Zhong-Xing , Fan, Xiao-Yan . Impacts of combined pollution under gradient increasing and gradient decreasing exposure modes on activated sludge: Microbial communities and antibiotic resistance genes . | BIORESOURCE TECHNOLOGY , 2021 , 345 . |
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摘要 ：
This study aimed to investigate the short-term response of abundant-rare genera and antibiotic resistance genes (ARGs) to azithromycin (AZM, 0.05-40 mg/L) and copper (1 mg/L) combined pollution in activated sludge nitrification system at low temperature. Nitrification was as expected inhibited in stress-and post-effects periods under AZM concentration higher than 5 mg/L. Abundant and rare taxa presented dissimilar responses based on full-scale classification. Conditionally rare or abundant taxa (CRAT) were keystone taxa. Relative abundance of ammonia-oxidizing archaea increased, and three aerobic denitrifying bacteria (Brevundimonas, Comamonas and Trichococcus) were enriched (from 9.83% to 68.91% in total). Ammonia nitrogen assimilating into Org-N and denitrification may be nitrogen pathways based on predict analysis. 29 ARGs were found with more co-occurrence patterns and high concentration of AZM (greater than 5 mg/L) caused their proliferation. Impor-tantly, expect for some abundant taxa, rare taxa, potential pathogens and nitrogen-removal functional genera were the main potential hosts of ARGs.
关键词 ：
Antibiotic resistance genes Antibiotic resistance genes Combined pollution Combined pollution Abundant-rare genera Abundant-rare genera Stress and post effects Stress and post effects Interactions of sludge microbiome Interactions of sludge microbiome
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| GB/T 7714 | Gao, Yu-Xi , Li, Xing , Zhao, Jun-Ru et al. Response of microbial communities based on full-scale classification and antibiotic resistance genes to azithromycin and copper combined pollution in activated sludge nitrification laboratory mesocosms at low temperature [J]. | BIORESOURCE TECHNOLOGY , 2021 , 341 . |
| MLA | Gao, Yu-Xi et al. "Response of microbial communities based on full-scale classification and antibiotic resistance genes to azithromycin and copper combined pollution in activated sludge nitrification laboratory mesocosms at low temperature" . | BIORESOURCE TECHNOLOGY 341 (2021) . |
| APA | Gao, Yu-Xi , Li, Xing , Zhao, Jun-Ru , Zhang, Zhong-Xing , Fan, Xiao-Yan . Response of microbial communities based on full-scale classification and antibiotic resistance genes to azithromycin and copper combined pollution in activated sludge nitrification laboratory mesocosms at low temperature . | BIORESOURCE TECHNOLOGY , 2021 , 341 . |
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摘要 ：
Vacuum ultraviolet/persulfate (VUV/PS) has been a prospective method for antibiotics removal, however, the enhanced degradation mechanism is still unclear. This study compared the sulfamethazine (SMT) degradation by VUV/PS and ultraviolet/persulfate (UV/PS) processes using a photoreaction system. Results showed that the SMT degradation rate and PS activation rate by VUV/PS was 1.36 times and 1.39 times those of the conventional UV/PS process under the same dosage of PS, respectively. VUV/PS also achieved a high SMT mineralization. SO4 center dot(-) and HO center dot played important roles in the degradation of SMT by both processes, while the role of center dot O-2(-) was little. SMT concentration changed the distributions of 185 nm and 254 nm photons absorbed by solution components and altered the initiated photolysis reaction. Both processes had faster degradation rates at low initial SMT concentrations and the optimum reaction condition was at solution pH of 5. The inhibited level of the SMT degradation in both processes by four anions was in the following order: NO3- > Cl- > HCO3- > SO42-. VUV/PS process was also more efficient than UV/PS for degrading environmental (200 mu g/L) and high (5 mg/L) concentration of SMT from three kinds of typical water matrices (tap, river and lake water). Finally, ten photo-products of SMT degradation by VUV (or UV) were identified, which were mainly the oxidized products of C-N, -NH2 and S-C on SMT molecules, and four degradation pathways were proposed. Overall, these findings could provide useful insights into the application of VUV/PS in efficient antibiotics removal from water.
关键词 ：
Vacuum ultraviolet Vacuum ultraviolet Ultraviolet Ultraviolet Persulfate Persulfate Sulfamethazine Sulfamethazine Degradation pathway Degradation pathway
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| GB/T 7714 | Li, Hang , Yang, Yan-Ling , Li, Xing et al. Enhanced degradation mechanism of sulfamethazine by vacuum ultraviolet/persulfate [J]. | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2021 , 9 (6) . |
| MLA | Li, Hang et al. "Enhanced degradation mechanism of sulfamethazine by vacuum ultraviolet/persulfate" . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING 9 . 6 (2021) . |
| APA | Li, Hang , Yang, Yan-Ling , Li, Xing , Fan, Xiao-Yan , Wang, Nan . Enhanced degradation mechanism of sulfamethazine by vacuum ultraviolet/persulfate . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2021 , 9 (6) . |
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摘要 ：
一种Fe‑g‑C3N4多功能纳米复合材料制备方法，属于水处理领域。将尿素和六水合氯化铁溶解于无水乙醇中，搅拌蒸发后得到复合物；复合物在马弗炉中进行一次煅烧，降温研磨清洗后得到片状Fe‑g‑C3N4片状复合物；片状Fe‑g‑C3N4复合物置于管式炉中，进行二次煅烧热剥离，得到Fe‑g‑C3N4纳米复合物。本发明还公开上述制备方法制备所得复合材料及其相关应用方法。本发明制备得到的Fe‑g‑C3N4可以实现类芬顿/光催化氧化协同降解污染物，降低了光生电子‑空穴复合率，拓宽了适用的pH值范围。
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| GB/T 7714 | 杨艳玲 , 冀思扬 , 李星 et al. 一种Fe-g-C3N4多功能纳米复合材料制备方法 : CN202010057763.5[P]. | 2020-01-19 . |
| MLA | 杨艳玲 et al. "一种Fe-g-C3N4多功能纳米复合材料制备方法" : CN202010057763.5. | 2020-01-19 . |
| APA | 杨艳玲 , 冀思扬 , 李星 , 周志伟 , 樊晓燕 . 一种Fe-g-C3N4多功能纳米复合材料制备方法 : CN202010057763.5. | 2020-01-19 . |
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