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学者姓名：鲁理平
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摘要 ：
制备了金纳米粒子-氧化石墨烯(AuNPs-GO)复合物,采用透射电镜(TEM)和紫外-可见吸收光谱(UV-Vis)对其进行了表征。将此复合物修饰到玻碳电极(GCE)表面,再通过Au—S键将巯基修饰的富含鸟嘌呤的DNA链(T30695)自组装到电极表面的金纳米粒子上。T30695可与Pb~(2+)结合形成稳定的平行G-四链体,此G-四链体再与氯化血红素(Hemin)结合形成具有过氧化物酶活性的DNA酶(DNAzyme),基于此制得Pb~(2+)敏感的DNA酶电化学传感器。形成的DNA酶可催化H_2O_2氧化对苯二酚(HQ),实现电化学信号的放大和Pb~(2+)的高灵敏检测。在优化的实验条件下,在...
关键词 ：
DNA酶 DNA酶 方波伏安法 方波伏安法 铅离子(Ⅱ) 铅离子(Ⅱ) 传感器 传感器
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| GB/T 7714 | 杨瑞 , 康天放 , 鲁理平 et al. 基于DNA酶/AuNPs-GO传感器方波伏安法高灵敏检测Pb~(2+) [J]. | 分析化学 , 2021 , 49 (02) : 309-317 . |
| MLA | 杨瑞 et al. "基于DNA酶/AuNPs-GO传感器方波伏安法高灵敏检测Pb~(2+)" . | 分析化学 49 . 02 (2021) : 309-317 . |
| APA | 杨瑞 , 康天放 , 鲁理平 , 叶青 . 基于DNA酶/AuNPs-GO传感器方波伏安法高灵敏检测Pb~(2+) . | 分析化学 , 2021 , 49 (02) , 309-317 . |
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摘要 ：
基于MoS2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(II)
关键词 ：
溶出伏安法 溶出伏安法 二硫化钼 二硫化钼 氮掺杂石墨烯 氮掺杂石墨烯 镉 镉
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| GB/T 7714 | 于志辉 , 蒲渝文 , 鲁理平 et al. 基于MoS2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(II) [J]. | 于志辉 , 2021 , 40 (1) : 17-20 . |
| MLA | 于志辉 et al. "基于MoS2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(II)" . | 于志辉 40 . 1 (2021) : 17-20 . |
| APA | 于志辉 , 蒲渝文 , 鲁理平 , 分析试验室 . 基于MoS2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(II) . | 于志辉 , 2021 , 40 (1) , 17-20 . |
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摘要 ：
基于S-Cd和N-Cd的亲和作用,利用一锅法制备了MoS_2-掺氮石墨烯(NG)新型纳米复合材料。采用方波溶出伏安法实现了Cd~(2+)的灵敏测定。在最优实验条件下,Cd~(2+)的溶出峰电流与Cd~(2+)的浓度在0.01～20μmol/L范围内呈线性关系(R~2=0.996),检出限为2.50 nmol/L。该传感器可用于监测实际样品中Cd~(2+)。
关键词 ：
二硫化钼 二硫化钼 氮掺杂石墨烯 氮掺杂石墨烯 溶出伏安法 溶出伏安法 镉 镉
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| GB/T 7714 | 于志辉 , 蒲渝文 , 鲁理平 . 基于MoS_2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(II) [J]. | 分析试验室 , 2021 , 40 (01) : 17-20 . |
| MLA | 于志辉 et al. "基于MoS_2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(II)" . | 分析试验室 40 . 01 (2021) : 17-20 . |
| APA | 于志辉 , 蒲渝文 , 鲁理平 . 基于MoS_2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(II) . | 分析试验室 , 2021 , 40 (01) , 17-20 . |
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摘要 ：
本文基于氧化石墨烯(GO)、电化学还原氧化石墨烯(ERGO)和氮掺杂石墨烯(NG)三种石墨烯材料修饰的电极制备了镉离子(Cd2+)电化学传感器.利用循环伏安法和差分脉冲伏安法分析检测Cd2+,系统的比较了不同石墨烯材料修饰电极的电化学性质及检测效果.结果表明GO修饰的传感器在灵敏度、检测限和可重复性方面优于ERGO和NG,说明了石墨烯上含氧基团的存在提高了Cd2+检测的灵敏度.
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| GB/T 7714 | 金鑫 , 李惠惠 , 鲁理平 . 石墨烯中杂原子对镉离子检测性能的影响研究 [J]. | 化学研究与应用 , 2021 , 33 (9) : 1701-1705 . |
| MLA | 金鑫 et al. "石墨烯中杂原子对镉离子检测性能的影响研究" . | 化学研究与应用 33 . 9 (2021) : 1701-1705 . |
| APA | 金鑫 , 李惠惠 , 鲁理平 . 石墨烯中杂原子对镉离子检测性能的影响研究 . | 化学研究与应用 , 2021 , 33 (9) , 1701-1705 . |
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摘要 ：
基于S-Cd和N-Cd的亲和作用,利用一锅法制备了MoS,-掺氮石墨烯(NG)新型纳米复合材料.采用方波溶出伏安法实现了Cd2+的灵敏测定.在最优实验条件下,Cd2+的溶出峰电流与Cd2+的浓度在0.01～20 μmol/L范围内呈线性关系(R2 =0.996),检出限为2.50 nmol/L.该传感器可用于监测实际样品中Cd2+.
关键词 ：
二硫化钼 二硫化钼 氮掺杂石墨烯 氮掺杂石墨烯 溶出伏安法 溶出伏安法 镉 镉
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| GB/T 7714 | 于志辉 , 蒲渝文 , 鲁理平 . 基于MoS2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(Ⅱ) [J]. | 分析试验室 , 2021 , 40 (1) : 17-20 . |
| MLA | 于志辉 et al. "基于MoS2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(Ⅱ)" . | 分析试验室 40 . 1 (2021) : 17-20 . |
| APA | 于志辉 , 蒲渝文 , 鲁理平 . 基于MoS2/氮掺杂石墨烯纳米复合材料灵敏电化学传感器检测镉(Ⅱ) . | 分析试验室 , 2021 , 40 (1) , 17-20 . |
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摘要 ：
The composite of gold nanoparticles-graphene oxide (AuNPs-GO) was prepared and characterized by transmission electron microscope (TEM) and ultraviolet-visible(UV-vis) spectrophotometry, respectively. The composite was modified on the surface of a glassy carbon electrode (GCE). The mercapto-modified and guanine-rich DNA strand (T30695) was self-assembled on to gold nanoparticles on the surface of the electrode via Au-S bond. T30695 could combine with lead ions (Pb2+) to form a stable parallel G-quadruplex. The G-quadruplex further combined with hemin to form DNAzyme with peroxidase activity. On the basis of this, DNAzyme electrochemical sensor Pb2+ detection was prepared. The DNAzyme could catalyze the oxidation reaction of hydrogen peroxidetowardshydroquinone (HQ) to achieve the amplification of electrochemical signal and highly sensitive detection of Pb2+. Under the optimum conditions, the peak current of square-wave voltammetry (SWV) was linearly dependent on the logarithm of Pb2+ concentration ranging from 5. 0 x10(-9) mol/L to 1. 0 x10(-6) mol/L with a detection limit of 1. 0 x10(-9) mol/L (S/N = 3). The recovery of Pb2+ in real water sample was 96% -105%. The sensor possessed good reproducibility, stability and selectivity to Pb2+.
关键词 ：
DNAzyme DNAzyme Lead ion (II) Lead ion (II) Sensor Sensor Square-wave voltammetry Square-wave voltammetry
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| GB/T 7714 | Yang Rui , Fang Tian-Fang , Lu Li-Ping et al. Highly Sensitive Detection of Pb2+ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry [J]. | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2021 , 49 (2) : 309-317 . |
| MLA | Yang Rui et al. "Highly Sensitive Detection of Pb2+ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry" . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 49 . 2 (2021) : 309-317 . |
| APA | Yang Rui , Fang Tian-Fang , Lu Li-Ping , Ye Qing . Highly Sensitive Detection of Pb2+ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2021 , 49 (2) , 309-317 . |
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摘要 ：
Dopamine participates in many physiological and pathological processes. Dynamic monitoring of dopamine levels in the cytoplasm of a single living cell reflects not only the functional state of dopamine synthesis factors but also the processes of related neurodegenerative diseases. Due to the low content of cytoplasmic dopamine and the difficulty to keep cells alive during the operating process, the detection of cytoplasmic dopamine is still challenging. Herein, a solid-phase microextraction (SPME) technique integrated nanobiosensor was employed to trace and quantify dopamine concentration fluctuations in the cytoplasm of a single living cell. We designed a polypyrrole modified carbon fiber nanoprobe as a bifunctional nanoprobe that can extract cytoplasmic dopamine and then perform electrochemical detection. This bifunctional nanoprobe can detect 10 pmol/L extracted dopamine and detected a 60% decrease of the cytoplasmic dopamine concentration in a single living cell by K+ stimulation. This study allowed for the first time serially detecting cytoplasmic dopamine while keeping the target cell alive, which might yield a new method for research on dopamine neurotoxicity and the related drug action mechanisms for neurodegenerative disease.
关键词 ：
Cytoplasm Cytoplasm Dopamine Dopamine Electrochemistry Electrochemistry Nano-based biosensor Nano-based biosensor Solid-phase microextraction (SPME) Solid-phase microextraction (SPME)
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| GB/T 7714 | Chang Yaran , Chen Yongjia , Shao Yunlong et al. Solid-phase microextraction integrated nanobiosensors for the serial detection of cytoplasmic dopamine in a single living cell. [J]. | Biosensors & bioelectronics , 2021 , 175 : 112915 . |
| MLA | Chang Yaran et al. "Solid-phase microextraction integrated nanobiosensors for the serial detection of cytoplasmic dopamine in a single living cell." . | Biosensors & bioelectronics 175 (2021) : 112915 . |
| APA | Chang Yaran , Chen Yongjia , Shao Yunlong , Li Boye , Wu Yuanyuan , Zhang Wenmei et al. Solid-phase microextraction integrated nanobiosensors for the serial detection of cytoplasmic dopamine in a single living cell. . | Biosensors & bioelectronics , 2021 , 175 , 112915 . |
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摘要 ：
Cell membrane permeability is one of the main indicators of cytotoxicity and related to many critical biological pathways. Here, we determined the Cd2+-induced membrane permeability of human MCF-7 cells using ferrocene methanol molecular probes based on scanning electrochemical microscopy (SECM). The cell height and topography were examined with an impermeable Ru(NH3)(6)Cl-3 probe. The membrane permeability exhibited no significant changes when the Cd2+ incubation time was less than 2 h and its concentration was less than 40 mu M. The permeability increased when the Cd2+ concentration was greater than 60 mu M, or when the incubation time was longer than 3 h. From the combined 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and cytoskeleton imaging experiments, it was found that the changes occurred because the cells exhibited a defensive mode and their membranes contracted when treated with a low concentration of Cd2+ for a short time. However, the cell membranes were irreversibly damaged when the cytoskeleton structures were destroyed, and the cell activities decreased at high concentrations over long periods. Interestingly, through the comparison with an x-scan study, it was found that DPV technology shows a higher performance in the detection of changes in the membrane permeability. Using a combination of cytoskeleton fluorescence imaging and cell-viability tests, the effect of the cadmium metal on the cell membrane permeability can be explored deeper and more comprehensively. This study provides a new idea for exploring the changes in the cell membrane permeability and may be helpful for rapid evaluation of cytotoxicity.
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| GB/T 7714 | Zhang, Biao , Pan, Na , Fan, Xiaoyin et al. Real-time effects of Cd(ii) on the cellular membrane permeability [J]. | ANALYST , 2021 , 146 (19) : 5973-5979 . |
| MLA | Zhang, Biao et al. "Real-time effects of Cd(ii) on the cellular membrane permeability" . | ANALYST 146 . 19 (2021) : 5973-5979 . |
| APA | Zhang, Biao , Pan, Na , Fan, Xiaoyin , Lu, Liping , Wang, Xiayan . Real-time effects of Cd(ii) on the cellular membrane permeability . | ANALYST , 2021 , 146 (19) , 5973-5979 . |
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摘要 ：
Binding of a target by a probe for selective detection depends on the state of the probes on the sensing interface. Here, the hanging strand length of triple-helix DNA was used to form tripod probes immobilized via pi-pi interactions on a reduced graphene-oxide substrate. The spacing between the probes was adjusted by controlling the lengths of the tripod "feet" on the substrate; that is, increased probe spacing occurred when foot size increased over the range of 6-12 bases. The surface coverages and electron-transfer rates mediated the tripod DNA probes were characterized by electrochemical methods and atomic force microscopy. The electron-transfer mediated by the tripod DNA probes was higher than that mediated by doubled-stranded DNA. Then different sizes tripod DNA probes were developed for protein-CEA detection. The DNA probes with 10 bases feet showed the best detection limit of detection of 10(-6) ng/mL in the detection linear range (10(-6) -25 ng/mL). The result demonstrated the tripod DNA probes with different sizes could obtain excellent sensitivity when it applied to the target with appropriate size. This interspace-controlled biosensing interface of tripod DNA probes with enhanced charge transfer should find widespread applications in clinical, medical, biological, and environmental areas for precise detection of differently sized targets.
关键词 ：
Carcinoembryonic antigen (CEA) Carcinoembryonic antigen (CEA) Charge transfer Charge transfer DNA sensor DNA sensor Electrochemistry Electrochemistry Interface Interface Triplex DNA Triplex DNA
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| GB/T 7714 | Jin, Xin , Lu, Liping , Wang, Xiayan . Interspace-controlled biosensing interface with enhanced charge transfer based on tripod DNA probes [J]. | TALANTA , 2021 , 234 . |
| MLA | Jin, Xin et al. "Interspace-controlled biosensing interface with enhanced charge transfer based on tripod DNA probes" . | TALANTA 234 (2021) . |
| APA | Jin, Xin , Lu, Liping , Wang, Xiayan . Interspace-controlled biosensing interface with enhanced charge transfer based on tripod DNA probes . | TALANTA , 2021 , 234 . |
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摘要 ：
Based on the strategy of increasing the number of oxygen vacancies to improve the catalytic performance, we have developed a novel electrochemical sensor based on the multivalent metal oxides cerium dioxide and manganous oxide (Mn3O4/CeO2) for reliable determination of extracellular hydrogen peroxide (H2O2) released from living cells. The Mn3O4/CeO2 nanocomposite was characterized by high-resolution transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The electrochemical performance of the Mn3O4/CeO2 nanocomposite modified glassy carbon electrode (Mn3O4/CeO2/GCE) was investigated. Owing to the abundant oxygen vacancies and strong synergistic effect between the multivalent Ce and Mn, the sensor exhibited excellent catalytic activity and selectivity for the electrochemical detection of H2O2 with a low quantitation limit of 2 nM. Moreover, Mn3O4/CeO2/GCE exhibited excellent reproducibility, repeatability, and long-term storage stability. Because of these remarkable analytical advantages, the constructed sensor was able to determine H2O2 released from living cells with satisfactory results. The results showed that the Mn3O4/CeO2 sensor is a promising candidate for a nanoenzymatic H2O2 sensor with the possibility of applications in physiology and diagnosis.
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| GB/T 7714 | Wu Yalin , Lu Liping , Yu Zhihui et al. Electrochemical sensor based on the Mn3O4/CeO2 nanocomposite with abundant oxygen vacancies for highly sensitive detection of hydrogen peroxide released from living cells. [J]. | Analytical methods : advancing methods and applications , 2021 , 13 (14) : 1672-1680 . |
| MLA | Wu Yalin et al. "Electrochemical sensor based on the Mn3O4/CeO2 nanocomposite with abundant oxygen vacancies for highly sensitive detection of hydrogen peroxide released from living cells." . | Analytical methods : advancing methods and applications 13 . 14 (2021) : 1672-1680 . |
| APA | Wu Yalin , Lu Liping , Yu Zhihui , Wang Xiayan . Electrochemical sensor based on the Mn3O4/CeO2 nanocomposite with abundant oxygen vacancies for highly sensitive detection of hydrogen peroxide released from living cells. . | Analytical methods : advancing methods and applications , 2021 , 13 (14) , 1672-1680 . |
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