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学者姓名：周志祥
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摘要 ：
为了进一步揭示PM_(2.5)暴露对肺的毒性损伤作用,本工作采用16HBE人肺支气管细胞,检测了PM_(2.5)暴露后16HBE的细胞活性、细胞中铁含量、GSH含量、LPO和MDA的产生情况.结果显示,PM_(2.5)(200μg·mL~(-1))处理16HBE细胞24 h后可导致细胞存活率下降、细胞铁含量增加、细胞内LPO和MDA生成量增加、GSH含量降低,而铁死亡抑制剂DFOM (6.25μmol·L~(-1))及Fer-1 (12.50μmol·L~(-1))可以显著减轻PM_(2.5)对细胞的毒性损伤作用,抑制MDA的产生,减少GSH的损耗.透射电子显微镜的形态学观察显示,PM_(2....
关键词 ：
PM_(2.5) PM_(2.5) 人支气管上皮细胞 人支气管上皮细胞 脂质过氧化 脂质过氧化 铁死亡 铁死亡
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| GB/T 7714 | 冯董董 , 洪启浩 , 李疆帅 et al. PM_(2.5)暴露诱发人支气管上皮细胞16HBE铁死亡 [J]. | 环境科学学报 , 2021 , 41 (09) : 3857-3864 . |
| MLA | 冯董董 et al. "PM_(2.5)暴露诱发人支气管上皮细胞16HBE铁死亡" . | 环境科学学报 41 . 09 (2021) : 3857-3864 . |
| APA | 冯董董 , 洪启浩 , 李疆帅 , 李文可 , 牛冰羽 , 王碧琪 et al. PM_(2.5)暴露诱发人支气管上皮细胞16HBE铁死亡 . | 环境科学学报 , 2021 , 41 (09) , 3857-3864 . |
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摘要 ：
Exposure to airborne particulate matter (PM2.5) is associated with cardiovascular diseases. In order to investigate the molecular mechanisms of air pollution-induced CVDs toxicity, human umbilical vein endothelial cells (HUVECs) were exposed to PM2.5 collected from January, 2016 winter in Beijing, China. We performed RNA sequencing to elucidate key molecular mechanism of PM 2.5-mediated toxicity in HUVECs. A total of 1753 genes, 864 up-regulated and 889 down-regulated, were observed to be differentially expressed genes (DEGs). Among these, genes involved in metabolic response, oxidative stress, inflammatory response, and vascular dysfunction were significantly differentially expressed (log2 FC > 4). The results were validated by quantitative real-time PCR (qPCR) and Western blot for CYP1B1, HMOX1, IL8, and GJA4. Pathway analysis revealed that DEGs were involved in the biological processes related to metabolism, inflammation, and host defense against environmental insults. This research is providing a further understanding of the mechanisms underlying PM2.5-induced cardiovascular diseases (CVDs).
关键词 ：
5 5 CVDs CVDs HUVEC HUVEC PM2 PM2 RNA-seq RNA-seq
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| GB/T 7714 | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis [J]. | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 . |
| MLA | Zhou, Zhixiang et al. "Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis" . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH (2021) . |
| APA | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara , Li, Wenke , Niu, Bingyu , Li, Jiangshuai et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 . |
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摘要 ：
在课程中融入思政教育是实现"三全育人"的关键途径.拓宽创新性教育的视野,结合工科院校分子肿瘤学课程的特点和优势,深层次挖掘思政元素,通过修订课程教学目标、创新教学模式、改革考核评价机制,将思政教育引入课堂教学中,实现知识传授与价值引领的有机统一,为培养和引导研究生树立正确的人生观和价值观奠定了基础.
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| GB/T 7714 | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 [J]. | 现代职业教育 , 2021 , (42) : 20-21 . |
| MLA | 张芳 et al. "新工科背景下研究生课程分子肿瘤学思政建设初探" . | 现代职业教育 42 (2021) : 20-21 . |
| APA | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 . | 现代职业教育 , 2021 , (42) , 20-21 . |
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摘要 ：
Oesophageal cancer is one of the deadliest cancers in the world. Oesophageal squamous cell carcinoma (ESCC) is the most prevalent histological type of oesophageal cancer. Oesophageal cancer has a poor prognosis because of its invasiveness. Thus, it is especially important to seek effective treatment methods. Research indicates that long non-coding RNAs (lncRNAs) play a significant role in the occurrence and development of oesophageal cancer. The aim of this study was to describe the role of LINC00958 in ESCC. Bioinformatics and real-time quantitative polymerase chain reaction (RT-qPCR) methods were utilized to predict and verify the expression of LINC00958 in ESCC. Related functional experiments, including cell proliferation, migration and invasion, were performed. In addition, a western blot and a dual luciferase reporter gene experiment were used to study the detailed carcinogenic mechanism of LINC00958. The results indicated there was a high expression of LINC00958 in ESCC, which promoted proliferation, migration, invasion and Epithelial-Mesenchymal Transition (EMT) of ESCC cells, and this effect may be via regulating miR-510-5p.
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| GB/T 7714 | Wang Biqi , Tang Duo , Liu Zijia et al. LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells. [J]. | PloS one , 2021 , 16 (5) : e0251797 . |
| MLA | Wang Biqi et al. "LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells." . | PloS one 16 . 5 (2021) : e0251797 . |
| APA | Wang Biqi , Tang Duo , Liu Zijia , Wang Qian , Xue Shan , Zhao Zijie et al. LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells. . | PloS one , 2021 , 16 (5) , e0251797 . |
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摘要 ：
Antibiotic resistance genes (ARGs) were considered as emerging genetic contaminants, which were threatening public health worldwide. ARGs had two forms of existence: intracellular ARGs (i-ARGs) and extracellular ARGs (e-ARGs), and their emergence and spread patterns were rarely revealed in aerobic granular sludge (AGS) system. The results showed that both i-ARGs and e-ARGs were enriched but had different shift patterns during AGS cultivation process, and e-ARGs had higher growth rate. Most functional bacteria enriched in AGS might play important roles in accumulating of i/e-ARGs, and their oligotypes might be the key reason for the different correlations between taxa and i/e-ARGs. E-ARGs might be transformed from i-ARGs and mainly carried by eintegrons, and three major modules of co-occurrence among e-ARGs were observed. The combined action of environmental factors and bacterial community contributed most (35.43%) to the shift pattern of e-ARGs. This study deciphered the migrations and accumulations of i/e-ARGs during aerobic granulation process, and provided several meaningful comprehensions on i/e-ARGs dissemination during practical AGS application.
关键词 ：
Aerobic granulation process Aerobic granulation process Co-occurrence modules Co-occurrence modules e-antibiotic resistance genes e-antibiotic resistance genes Functional bacteria Functional bacteria I I Oligotypes Oligotypes
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| GB/T 7714 | Li, Dingchang , Gao, Jingfeng , Dai, Huihui et al. Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process [J]. | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
| MLA | Li, Dingchang et al. "Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process" . | CHEMICAL ENGINEERING JOURNAL 421 (2021) . |
| APA | Li, Dingchang , Gao, Jingfeng , Dai, Huihui , Duan, Wanjun , Wang, Zhiqi , Zhou, Zhixiang . Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process . | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
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摘要 ：
为研究肺水通道蛋白1(AQP1)在PM2.5暴露引起肺损伤中的作用,本文首先在细胞水平上检测了PM2.5暴露对肺上皮细胞A549的细胞存活率、细胞AQP1的mRNA和蛋白表达变化,以及AQP1在细胞内的表达定位的影响.另外,将10只雄性SD大鼠随机分为2组:对照组和PM2.5染毒组(1.5 mg·kg-1体重),每组5只,采用气管滴注法染毒,每隔1d染毒1次,共2周,制备PM2.5暴露大鼠模型,进一步观察PM2.5暴露对大鼠肺AQP1表达及肺组织损伤的影响情况.结果显示,PM2.5暴露可引起肺上皮细胞A549形态异常和存活率降低.PM2.5暴露后的24 h内A549细胞的AQP1 mRNA及蛋白相对表达水平先升高,并在暴露后的12h内达到最高水平,然后有所下降;免疫荧光结果也证实AQP1蛋白在PM25暴露后表达水平升高,并呈现从细胞质向细胞膜定位的过程;用PM2.5暴露处理SD大鼠肺组织中AQP1表达亦显著增加,并且肺组织出现肺泡隔增厚,并有广泛的水肿充血及炎症细胞浸润.总之,PM2.5暴露可诱导A549细胞及大鼠肺组织内AQP1的表达升高,可能参与PM2.5暴露引起的肺病理性损伤过程.
关键词 ：
AQP1 AQP1 PM25 PM25 急性肺损伤 急性肺损伤 肺上皮细胞 肺上皮细胞
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| GB/T 7714 | 李文可 , 牛冰羽 , 李疆帅 et al. PM2.5暴露对肺AQP1表达的影响研究 [J]. | 环境科学学报 , 2020 , 40 (6) : 2255-2261 . |
| MLA | 李文可 et al. "PM2.5暴露对肺AQP1表达的影响研究" . | 环境科学学报 40 . 6 (2020) : 2255-2261 . |
| APA | 李文可 , 牛冰羽 , 李疆帅 , 洪启浩 , 苗晓燕 , 周志祥 . PM2.5暴露对肺AQP1表达的影响研究 . | 环境科学学报 , 2020 , 40 (6) , 2255-2261 . |
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摘要 ：
使用冬季北京市城区的细颗粒物(PM2.5)评估其对人肺上皮细胞系A549中多环芳烃受体(AhR)通路的激活作用.利用CCK-8法检测细胞暴露PM2.5后的存活率,选取50％抑制浓度(IC50)作为细胞暴露剂量,采用Western blot和免疫荧光检测PM2.5暴露诱导AhR定位变化,采用荧光定量PCR和Westem blot检测AhR的靶基因CYP1A1的转录和翻译水平改变,应用双荧光素酶报告基因法测定AhR与靶基因结合位点(XRE)的活性,利用酶标仪测定CYP1A1的酶活性改变.结果显示,随着暴露时间的增加,AhR逐渐从细胞质易位至细胞核;CYP1A1 mRNA和蛋白质的表达水平也呈时间依赖性显著增加;AhR与XRE结合活性的增加表明了PM2.5诱导CYP1A1增加的调节机制;最后引起细胞内CYP1A1酶活性显著增加.研究表明,来自冬季北京城区的PM2.5样品可激活A549细胞的AhR通路,提示AhR介导的信号途径可能与PM2.5的暴露毒性有关.
关键词 ：
CYP1A1 CYP1A1 PM2.5 PM2.5 人肺上皮细胞 人肺上皮细胞 多环芳烃受体 多环芳烃受体
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| GB/T 7714 | 牛冰羽 , 李文可 , 苗晓燕 et al. 冬季北京城区PM2.5诱导人肺上皮细胞系A549中多环芳烃受体(AhR)通路的激活 [J]. | 环境科学学报 , 2020 , 40 (7) : 2652-2658 . |
| MLA | 牛冰羽 et al. "冬季北京城区PM2.5诱导人肺上皮细胞系A549中多环芳烃受体(AhR)通路的激活" . | 环境科学学报 40 . 7 (2020) : 2652-2658 . |
| APA | 牛冰羽 , 李文可 , 苗晓燕 , 李疆帅 , 洪启浩 , Sara Khodahemmati et al. 冬季北京城区PM2.5诱导人肺上皮细胞系A549中多环芳烃受体(AhR)通路的激活 . | 环境科学学报 , 2020 , 40 (7) , 2652-2658 . |
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摘要 ：
Epidemiological studies have corroborated that respiratory diseases, including lung cancer, are related to fine particulate matter (<2.5 mu m) (PM2.5) exposure. The toxic responses of PM(2.5)are greatly influenced by the source of PM2.5. However, the effects of PM(2.5)from Beijing on bronchial genotoxicity are scarce. In the present study, PM(2.5)from Beijing was sampled and applied in vitro to investigate its genotoxicity and the mechanisms behind it. Human bronchial epithelial cells 16HBE were used as a model for exposure. Low (67.5 mu g/mL), medium (116.9 mu g/mL), and high (202.5 mu g/mL) doses of PM(2.5)were used for cell exposure. After PM(2.5)exposure, cell viability, oxidative stress markers, DNA (deoxyribonucleic acid) strand breaks, 8-OH-dG levels, micronuclei formation, and DNA repair gene expression were measured. The results showed that PM(2.5)significantly induced cytotoxicity in 16HBE. Moreover, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and cellular heme oxygenase (HO-1) were increased, and the level of glutathione (GSH) was decreased, which represented the occurrence of severe oxidative stress in 16HBE. The micronucleus rate was elevated, and DNA damage occurred as indicators of the comet assay, gamma-H2AX and 8-OH-dG, were markedly enhanced by PM2.5, accompanied by the influence of 8-oxoguanine DNA glycosylase (OGG1), X-ray repair cross-complementing gene 1 (XRCC1), and poly (ADP-ribose) polymerase-1 (PARP1) expression. These results support the significant role of PM(2.5)genotoxicity in 16HBE cells, which may occur through the combined effect on oxidative stress and the influence of DNA repair genes.
关键词 ：
5 5 DNA damage DNA damage DNA repair gene DNA repair gene human bronchial epithelial cells human bronchial epithelial cells oxidative stress oxidative stress PM2 PM2
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| GB/T 7714 | Niu, Bing-Yu , Li, Wen-Ke , Li, Jiang-Shuai et al. Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM(2.5)from Beijing, China, in Winter [J]. | INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH , 2020 , 17 (13) . |
| MLA | Niu, Bing-Yu et al. "Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM(2.5)from Beijing, China, in Winter" . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 17 . 13 (2020) . |
| APA | Niu, Bing-Yu , Li, Wen-Ke , Li, Jiang-Shuai , Hong, Qi-Hao , Khodahemmati, Sara , Gao, Jing-Feng et al. Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM(2.5)from Beijing, China, in Winter . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH , 2020 , 17 (13) . |
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摘要 ：
Immunotherapeutic strategies based on Epstein-Barr virus (EBV) latent membrane protein 2 (LMP2) antigen-specific cytotoxic T lymphocytes (CTLs) have been proven to boost LMP2-specific CTL responses in patients with nasopharyngeal carcinoma (NPC). Such strategies can produce clinical benefits in some patients with NPC. Currently, the major challenge limiting the use of immunotherapy for NPC is its low clinical response rate. The efficacy of immunotherapy based on EBV-LMP2 specific CTLs depends mainly on their cytotoxic activity, but no studies have been conducted to elucidate this activity. In this study, laser confocal scanning microscopy (LCSM) and real-time cell analysis (RTCA) were used to evaluate the killing function and its underlying mechanism of LMP2-specific CTLs. LCSM showed that LMP2-specific CTLs recognize and kill target cells expressing viral escape protein LMP2, and that the killing rate is related to the number of CTLs adhering to the target cells. LMP2-specific CTL-mediated cytotoxicity is rate limited by the time required for effective contact and recognition between CTLs and target cells. RTCA showed that the protective effect of LMP2-specific CTLs required an appropriate effector-to-target ratio, and that LMP2-specific CTLs could not eradicate residual target cells at a low effector-to-target ratio. Moreover, our results revealed that LMP2-specific CTL responses involve two independent but complementary mechanisms: the perforin/granzyme and Fas/FasL pathways. Therefore, we have elucidated, for the first time, the selective cytotoxicity and mechanism by which LMP2-specific CTLs induced by the rAd-LMP2 vaccine kill target cells and have explored the killing mode and several key parameters of killing mediated by LMP2-specific CTLs. Our study will contribute to the knowledge of vaccines targeting EBV-LMP2 and to the improvement of immunotherapeutic strategies.
关键词 ：
Cytotoxic T lymphocytes Cytotoxic T lymphocytes Epstein-Barr virus Epstein-Barr virus Immunotherapy Immunotherapy Latent membrane protein 2 Latent membrane protein 2 Nasopharyngeal carcinoma Nasopharyngeal carcinoma Vaccine Vaccine
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| GB/T 7714 | Ge, Yuyang , Zhou, Zhixiang , Wang, Xiaoli et al. In vitro evaluation of the therapeutic effectiveness of EBV-LMP2 recombinant adenovirus vaccine in nasopharyngeal carcinoma [J]. | BIOMEDICINE & PHARMACOTHERAPY , 2020 , 121 . |
| MLA | Ge, Yuyang et al. "In vitro evaluation of the therapeutic effectiveness of EBV-LMP2 recombinant adenovirus vaccine in nasopharyngeal carcinoma" . | BIOMEDICINE & PHARMACOTHERAPY 121 (2020) . |
| APA | Ge, Yuyang , Zhou, Zhixiang , Wang, Xiaoli , Zhou, Yubai , Liu, Wei , Teng, Zhiping et al. In vitro evaluation of the therapeutic effectiveness of EBV-LMP2 recombinant adenovirus vaccine in nasopharyngeal carcinoma . | BIOMEDICINE & PHARMACOTHERAPY , 2020 , 121 . |
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摘要 ：
Background: Esophageal cancer is one of the most common malignant tumours in humans. A series of esophageal cancer cell lines are accompanied by human papilloma virus (HPV) infection, but the mechanism behind HPV in cancer malignancy is not clear. Methods: This research was conducted in different generations of HPV E6E7 gene-induced human foetal esophageal epithelial immortalised cells (Shantou Human Embryonic Esophageal Epithelial cell line; SHEE); the RNA sequencing transcriptomic analysis was performed to explore the mechanism of HPV infection in these cell lines. Results: The results showed that there are 9,990 differential genes in late-stage cells compared with HPV18 E6E7-infected early foetal esophageal epithelial immortalised cells. Among these, 4,882 genes are upregulated, and 5,108 genes are downregulated. We used bioinformatics to analyze the expression and function of aberrantly expressed lncRNA, miRNA, mRNA and construct the competing endogenous RNA (ceRNA) network and protein protein interaction (PPI) network. Conclusions: we predicted TP53TG1 promotes to malignant transformation of SHEEs by acting as a ceRNA to competitively bind to miR-6835 and regulate IGF2 expression. We also predicted IL6 serve as prognostic biomarkers and therapy target. With these results maybe provides new insights into the mechanisms of HPV carcinogenesis in esophageal cancer.
关键词 ：
esophageal cancer esophageal cancer Human papilloma virus (HPV) Human papilloma virus (HPV) qRT-PCR qRT-PCR RNA-Seq RNA-Seq
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| GB/T 7714 | Tang, Duo , Wang, Biqi , Khodahemmati, Sara et al. A transcriptomic analysis of malignant transformation of human embryonic esophageal epithelial cells by HPV18 E6E7 [J]. | TRANSLATIONAL CANCER RESEARCH , 2020 , 9 (3) : 1818-1832 . |
| MLA | Tang, Duo et al. "A transcriptomic analysis of malignant transformation of human embryonic esophageal epithelial cells by HPV18 E6E7" . | TRANSLATIONAL CANCER RESEARCH 9 . 3 (2020) : 1818-1832 . |
| APA | Tang, Duo , Wang, Biqi , Khodahemmati, Sara , Li, Jingtao , Zhou, Zhixiang , Gao, Jingfeng et al. A transcriptomic analysis of malignant transformation of human embryonic esophageal epithelial cells by HPV18 E6E7 . | TRANSLATIONAL CANCER RESEARCH , 2020 , 9 (3) , 1818-1832 . |
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