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学者姓名：周志祥
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摘要 ：
Background Although esophageal carcinoma (EC) is one of the most common cancers in the world, details of its pathogenesis remain unclear. Metabolic reprogramming is a main feature of EC. Mitochondrial dysfunction, especially the decrease in mitochondrial complex I (MTCI), plays an important role in the occurrence and development of EC.Objective The objective of the study was to analyze and validate the metabolic abnormalities and the role of MTCI in esophageal squamous cell carcinoma.Methods In this work, we collected transcriptomic data from 160 esophageal squamous carcinoma samples and 11 normal tissue samples from The Cancer Genome Atlas (TCGA). The OmicsBean and GEPIA2 were used to conduct an analysis of differential gene expression and survival in clinical samples. Rotenone was used to inhibit the MTCI activity. Subsequently, we detected lactate production, glucose uptake, and ATP production.Results A total of 1710 genes were identified as being significantly differentially expressed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analysis suggested that these differentially expressed genes (DEGs) were significantly enriched in various pathways related to carcinoma tumorigenesis and progression. Moreover, we further identified abnormalities in metabolic pathways, in particular, the significantly low expression of multiple subunits of MTCI genes (ND1, ND2, ND3, ND4, ND4L, ND5, and ND6). Rotenone was used to inhibit the MTCI activity of EC109 cells, and it was found that the decrease in MTCI activity promoted HIF1A expression, glucose consumption, lactate production, ATP production, and cell \nmigration.Conclusion Our results indicated the occurrence of abnormal metabolism involving decreased mitochondrial complex I activity and increased glycolysis in esophageal squamous cell carcinoma (ESCC), which might be related to its development and degree of malignancy.
关键词 ：
Esophageal carcinoma (EC) Esophageal carcinoma (EC) mitochondrial complex I (MTCI) mitochondrial complex I (MTCI) mitochondrial dysfunction mitochondrial dysfunction bioinformatics analysis bioinformatics analysis rotenone rotenone esophageal squamous cell carcinoma (ESCC) esophageal squamous cell carcinoma (ESCC)
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| GB/T 7714 | Tang, Duo , Wang, Guozhen , Liu, Zijia et al. Bioinformatics Analysis and Verification of Metabolic Abnormalities in Esophageal Squamous Carcinoma [J]. | COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING , 2024 , 27 (2) : 273-283 . |
| MLA | Tang, Duo et al. "Bioinformatics Analysis and Verification of Metabolic Abnormalities in Esophageal Squamous Carcinoma" . | COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING 27 . 2 (2024) : 273-283 . |
| APA | Tang, Duo , Wang, Guozhen , Liu, Zijia , Zheng, Yu Chen , Sheng, Chao , Wang, Biqi et al. Bioinformatics Analysis and Verification of Metabolic Abnormalities in Esophageal Squamous Carcinoma . | COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING , 2024 , 27 (2) , 273-283 . |
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| GB/T 7714 | Zong, Qi , Wang, Guozhen , Zhao, Zijie et al. Fabrication and characterization of dissolving microneedles for transdermal delivery of hypocrellin A (vol 95, 105594, 2024) [J]. | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY , 2024 , 101 . |
| MLA | Zong, Qi et al. "Fabrication and characterization of dissolving microneedles for transdermal delivery of hypocrellin A (vol 95, 105594, 2024)" . | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 101 (2024) . |
| APA | Zong, Qi , Wang, Guozhen , Zhao, Zijie , Li, Wenzhuo , Hou, Xiaonan , Yao, Mengfei et al. Fabrication and characterization of dissolving microneedles for transdermal delivery of hypocrellin A (vol 95, 105594, 2024) . | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY , 2024 , 101 . |
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摘要 ：
Micro(nano)plastics (MNPs) are emerging pollutants that can adsorb pollutants in the environment and biological molecules and ultimately affect human health. However, the aspects of adsorption of intracellular proteins onto MNPs and its biological effects in cells have not been investigated to date. The present study revealed that 100 nm polystyrene nanoplastics (NPs) could be internalized by THP-1 cells and specifically adsorbed intracellular proteins. In total, 773 proteins adsorbed onto NPs with high reliability were identified using the proteomics approach and analyzed via bioinformatics to predict the route and distribution of NPs following cellular internalization. The representative proteins identified via the Kyoto Encyclopedia of Genes and Genomes pathway analysis were further investigated to characterize protein adsorption onto NPs and its biological effects. The analysis revealed that NPs affect glycolysis through pyruvate kinase M (PKM) adsorption, trigger the unfolded protein response through the adsorption of ribophorin 1 (RPN1) and heat shock 70 protein 8 (HSPA8), and are chiefly internalized into cells through clathrin-mediated endocytosis with concomitant clathrin heavy chain (CLTC) adsorption. Therefore, this work provides new insights and research strategies for the study of the biological effects caused by NPs.
关键词 ：
proteomics proteomics nanoplastics nanoplastics emerging pollutants emerging pollutants protein adsorption protein adsorption bioinformatics analysis bioinformatics analysis
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| GB/T 7714 | Liu, Zijia , Wang, Guozhen , Sheng, Chao et al. Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells [J]. | ENVIRONMENTAL SCIENCE & TECHNOLOGY , 2024 , 58 (6) : 2652-2661 . |
| MLA | Liu, Zijia et al. "Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells" . | ENVIRONMENTAL SCIENCE & TECHNOLOGY 58 . 6 (2024) : 2652-2661 . |
| APA | Liu, Zijia , Wang, Guozhen , Sheng, Chao , Zheng, Yuchen , Tang, Duo , Zhang, Yuchen et al. Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells . | ENVIRONMENTAL SCIENCE & TECHNOLOGY , 2024 , 58 (6) , 2652-2661 . |
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摘要 ：
Hypocrellin A (HA) is an efficient photosensitizer for photodynamic therapy in recent years. In order to improve the delivery of this photosensitizer, dissolving HA microneedles were established. Firstly, dissolving microneedles were prepared from HA by a two-step solvent casting method. Then, the dissolving HA microneedle were characterized by stereomicroscope, laser confocal microscope, scanning electron microscope, differential scanning calorimeter (DSC), X-ray diffractometer (XRD) and fourier infrared spectrometer (FTIR). Finally, the transdermal cumulative penetration experiments were conducted. The results showed that the dissolving HA microneedle has a conical shape with an average height of 650 mu m. The mechanical strength endure a force of 44.78 N, resulting in 0.20 N per needle. The in vitro transdermal experiment showed that the dissolving HA microneedle almost completely dissolved in 5 min after insertion into the mouse skin. The depth of drug penetration through the skin can reach 601.36 mu m. Moreover, the dissolving HA microneedle significantly improved the cumulative transdermal penetration efficiency of the photosensitizer through the skin. Therefore, the present study successfully established a dissolving microneedle of HA, which can quickly dissolve and significantly improve the transdermal delivery efficiency of HA.
关键词 ：
Hypocrellin A Hypocrellin A Dissolving microneedles Dissolving microneedles Photosensitizer Photosensitizer Transdermal Transdermal
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| GB/T 7714 | Zong, Qi , Wang, Guozhen , Zhao, Zijie et al. Fabrication and characterization of dissolving microneedles for transdermal delivery of hypocrellin A [J]. | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY , 2024 , 95 . |
| MLA | Zong, Qi et al. "Fabrication and characterization of dissolving microneedles for transdermal delivery of hypocrellin A" . | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 95 (2024) . |
| APA | Zong, Qi , Wang, Guozhen , Zhao, Zijie , Li, Wenzhuo , Hou, Xiaonan , Yao, Mengfei et al. Fabrication and characterization of dissolving microneedles for transdermal delivery of hypocrellin A . | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY , 2024 , 95 . |
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摘要 ：
本发明涉及一种跨上皮/内皮细胞电阻仪及其测量方法，其主控板的输入端与固定电阻和测试电极并联，主控板的电源端与外部供电电源连接；测试电极采用银线电极。采用细胞悬浮液进行铺板，待细胞贴壁后，将内室含血清的培养基采用无血清的DMEM溶液替换，外室内的溶液也采用无血清的DMEM溶液替换；由具有银线电极的跨上皮/内皮细胞电阻仪测量空白孔的电阻值，并将空白孔的内室和外室均换成DMEM溶液，此时将Transwell小室转移到测板中；铺板完成后由具有银线电极的跨上皮/内皮细胞电阻仪连续多天测量内皮细胞的TEER值；将每天测量多个内皮细胞的TEER值求均值，根据每天测量结果的均值得到同细胞生长曲线具有一致趋势的TEER值的结果图，作为衡量上皮/内皮细胞屏障形成的定量标准。
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| GB/T 7714 | 周志祥 , 侯潇楠 , 姚梦菲 et al. 一种跨上皮/内皮细胞电阻仪及其测量方法 : CN202310597170.1[P]. | 2023-05-25 . |
| MLA | 周志祥 et al. "一种跨上皮/内皮细胞电阻仪及其测量方法" : CN202310597170.1. | 2023-05-25 . |
| APA | 周志祥 , 侯潇楠 , 姚梦菲 , 唐铎 , 刘紫佳 , 郑雨晨 et al. 一种跨上皮/内皮细胞电阻仪及其测量方法 : CN202310597170.1. | 2023-05-25 . |
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摘要 ：
Objective To establish a sensitive, simple and rapid detection method for African swine fever virus (ASFV) B646L gene. Methods A recombinase-aided amplification-lateral flow dipstick (RAA-LFD) assay was developed in this study. Recombinase-aided amplification (RAA) is used to amplify template DNA, and lateral flow dipstick (LFD) is used to interpret the results after the amplification is completed. The lower limits of detection and specificity of the RAA assay were verified using recombinant plasmid and pathogenic nucleic acid. In addition, 30 clinical samples were tested to evaluate the performance of the RAA assay. Results The RAA-LFD assay was completed within 15 min at 37 degrees C, including 10 min for nucleic acid amplification and 5 minutes for LFD reading results. The detection limit of this assay was found to be 200 copies per reaction. And there was no cross-reactivity with other swine viruses. Conclusion A highly sensitive, specific, and simple RAA-LFD method was developed for the rapid detection of the ASFV.
关键词 ：
Lateral flow detection Lateral flow detection African swine fever virus African swine fever virus Recombinase aided amplification Recombinase aided amplification
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| GB/T 7714 | Li Jiang Shuai , Hao Yan Zhe , Hou Mei Ling et al. Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus [J]. | BIOMEDICAL AND ENVIRONMENTAL SCIENCES , 2022 , 35 (2) : 133-140 . |
| MLA | Li Jiang Shuai et al. "Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus" . | BIOMEDICAL AND ENVIRONMENTAL SCIENCES 35 . 2 (2022) : 133-140 . |
| APA | Li Jiang Shuai , Hao Yan Zhe , Hou Mei Ling , Zhang Xuan , Zhang Xiao Guang , Cao Yu Xi et al. Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus . | BIOMEDICAL AND ENVIRONMENTAL SCIENCES , 2022 , 35 (2) , 133-140 . |
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摘要 ：
在课程中融入思政教育是实现"三全育人"的关键途径.拓宽创新性教育的视野,结合工科院校分子肿瘤学课程的特点和优势,深层次挖掘思政元素,通过修订课程教学目标、创新教学模式、改革考核评价机制,将思政教育引入课堂教学中,实现知识传授与价值引领的有机统一,为培养和引导研究生树立正确的人生观和价值观奠定了基础.
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| GB/T 7714 | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 [J]. | 现代职业教育 , 2021 , (42) : 20-21 . |
| MLA | 张芳 et al. "新工科背景下研究生课程分子肿瘤学思政建设初探" . | 现代职业教育 42 (2021) : 20-21 . |
| APA | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 . | 现代职业教育 , 2021 , (42) , 20-21 . |
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摘要 ：
Antibiotic resistance genes (ARGs) were considered as emerging genetic contaminants, which were threatening public health worldwide. ARGs had two forms of existence: intracellular ARGs (i-ARGs) and extracellular ARGs (e-ARGs), and their emergence and spread patterns were rarely revealed in aerobic granular sludge (AGS) system. The results showed that both i-ARGs and e-ARGs were enriched but had different shift patterns during AGS cultivation process, and e-ARGs had higher growth rate. Most functional bacteria enriched in AGS might play important roles in accumulating of i/e-ARGs, and their oligotypes might be the key reason for the different correlations between taxa and i/e-ARGs. E-ARGs might be transformed from i-ARGs and mainly carried by eintegrons, and three major modules of co-occurrence among e-ARGs were observed. The combined action of environmental factors and bacterial community contributed most (35.43%) to the shift pattern of e-ARGs. This study deciphered the migrations and accumulations of i/e-ARGs during aerobic granulation process, and provided several meaningful comprehensions on i/e-ARGs dissemination during practical AGS application.
关键词 ：
I I Aerobic granulation process Aerobic granulation process Oligotypes Oligotypes Functional bacteria Functional bacteria e-antibiotic resistance genes e-antibiotic resistance genes Co-occurrence modules Co-occurrence modules
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| GB/T 7714 | Li, Dingchang , Gao, Jingfeng , Dai, Huihui et al. Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process [J]. | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
| MLA | Li, Dingchang et al. "Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process" . | CHEMICAL ENGINEERING JOURNAL 421 (2021) . |
| APA | Li, Dingchang , Gao, Jingfeng , Dai, Huihui , Duan, Wanjun , Wang, Zhiqi , Zhou, Zhixiang . Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process . | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
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摘要 ：
Exposure to airborne particulate matter (PM2.5) is associated with cardiovascular diseases. In order to investigate the molecular mechanisms of air pollution-induced CVDs toxicity, human umbilical vein endothelial cells (HUVECs) were exposed to PM2.5 collected from January, 2016 winter in Beijing, China. We performed RNA sequencing to elucidate key molecular mechanism of PM 2.5-mediated toxicity in HUVECs. A total of 1753 genes, 864 up-regulated and 889 down-regulated, were observed to be differentially expressed genes (DEGs). Among these, genes involved in metabolic response, oxidative stress, inflammatory response, and vascular dysfunction were significantly differentially expressed (log2 FC > 4). The results were validated by quantitative real-time PCR (qPCR) and Western blot for CYP1B1, HMOX1, IL8, and GJA4. Pathway analysis revealed that DEGs were involved in the biological processes related to metabolism, inflammation, and host defense against environmental insults. This research is providing a further understanding of the mechanisms underlying PM2.5-induced cardiovascular diseases (CVDs).
关键词 ：
5 5 CVDs CVDs RNA-seq RNA-seq PM2 PM2 HUVEC HUVEC
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| GB/T 7714 | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis [J]. | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 , 32 (9) : 2052-2064 . |
| MLA | Zhou, Zhixiang et al. "Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis" . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH 32 . 9 (2021) : 2052-2064 . |
| APA | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara , Li, Wenke , Niu, Bingyu , Li, Jiangshuai et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 , 32 (9) , 2052-2064 . |
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摘要 ：
Oesophageal cancer is one of the deadliest cancers in the world. Oesophageal squamous cell carcinoma (ESCC) is the most prevalent histological type of oesophageal cancer. Oesophageal cancer has a poor prognosis because of its invasiveness. Thus, it is especially important to seek effective treatment methods. Research indicates that long non-coding RNAs (lncRNAs) play a significant role in the occurrence and development of oesophageal cancer. The aim of this study was to describe the role of LINC00958 in ESCC. Bioinformatics and real-time quantitative polymerase chain reaction (RT-qPCR) methods were utilized to predict and verify the expression of LINC00958 in ESCC. Related functional experiments, including cell proliferation, migration and invasion, were performed. In addition, a western blot and a dual luciferase reporter gene experiment were used to study the detailed carcinogenic mechanism of LINC00958. The results indicated there was a high expression of LINC00958 in ESCC, which promoted proliferation, migration, invasion and Epithelial-Mesenchymal Transition (EMT) of ESCC cells, and this effect may be via regulating miR-510-5p.
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| GB/T 7714 | Wang, Biqi , Tang, Duo , Liu, Zijia et al. LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells [J]. | PLOS ONE , 2021 , 16 (5) . |
| MLA | Wang, Biqi et al. "LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells" . | PLOS ONE 16 . 5 (2021) . |
| APA | Wang, Biqi , Tang, Duo , Liu, Zijia , Wang, Qian , Xue, Shan , Zhao, Zijie et al. LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells . | PLOS ONE , 2021 , 16 (5) . |
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