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学者姓名：汪夏燕
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摘要 ：
Gold thin films have attracted a growing number attention because of their advantages such as easy preparation, conveniently subsequent modifications, good chemical stability, high electrical conductivity and low cytotoxicity. The preparation of continuous metal layer on capillary has become a classic leading step in the field of analytical chemistry. In recent years, researchers have established a variety of methods to prepare gold thin films, which has been applied in many fields. This paper reviews the preparation methods of gold thin films based on capillary, and its application in electrochemical sensing analysis, mainly discussing the technical characteristics and research status of electroless deposition, electrochemical deposition and physical vapor deposition. In addition, the development prospects of gold plating on capillary are also prospected.
关键词 ：
Gold thin films Gold thin films Electrochemical sensing analysis Electrochemical sensing analysis Review Review Capillary Capillary
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| GB/T 7714 | Cao Shi-Ting , Zhang Wen-Mei , Shao Yun-Long et al. Progress in Preparation of Gold Plating on Capillary for Electrochemical Sensor Analysis [J]. | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2021 , 49 (5) : 653-664 . |
| MLA | Cao Shi-Ting et al. "Progress in Preparation of Gold Plating on Capillary for Electrochemical Sensor Analysis" . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 49 . 5 (2021) : 653-664 . |
| APA | Cao Shi-Ting , Zhang Wen-Mei , Shao Yun-Long , Guo Guang-Sheng , Wang Xia-Yan . Progress in Preparation of Gold Plating on Capillary for Electrochemical Sensor Analysis . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2021 , 49 (5) , 653-664 . |
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摘要 ：
Intracellular delivery of materials with nanopipettes has become a critical method in gene editing and cell-based therapy because of its nanometer-scaled features capable of penetrating the cell membrane and targeting specific subcellular compartments-directing materials to the endoplasmic reticulum, nucleus, or mitochondria, for example. The geometrical parameters of nanopipettes directly affect delivery efficiency. However, the current methods for fabricating nanopipettes have the disadvantages of poor controllability, high cost, and difficult operation. To overcome these issues, we propose a real-time visualization method for fabricating nanopipettes based on pressured electrolyte chamber based wet etching to precisely determine the tip size during the fabrication process. A standard curve is plotted to provide a direction for fabrication, and tip inner diameters smaller than 10 nm can be controllably achieved. Furthermore, the intranuclear injection of proteins to living single cells (diameter < 30 mu m) with a high spatial resolution is realized. And single-cell transfection through the intracellular delivery of plasmid based on a self-built living single-cell workstation is completed. This technique is expected to be used in the treatment of diseases, for high-resolution localization of organelles in living single cells without fluorescent labeling, and for subcellular omics analysis by mass spectrometry.
关键词 ：
controllable fabrication controllable fabrication intracellular delivery intracellular delivery living single cell living single cell nanopipette nanopipette wet etching wet etching
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| GB/T 7714 | Wu, Yuanyuan , Shao, Yunlong , Zhang, Wenmei et al. Silica-Based Nanopipettes for Rapid Living Single-Cell Transfection [J]. | ACS APPLIED NANO MATERIALS , 2021 , 4 (7) : 6956-6963 . |
| MLA | Wu, Yuanyuan et al. "Silica-Based Nanopipettes for Rapid Living Single-Cell Transfection" . | ACS APPLIED NANO MATERIALS 4 . 7 (2021) : 6956-6963 . |
| APA | Wu, Yuanyuan , Shao, Yunlong , Zhang, Wenmei , Li, Boye , Zhao, Liang , Zhang, Dongtang et al. Silica-Based Nanopipettes for Rapid Living Single-Cell Transfection . | ACS APPLIED NANO MATERIALS , 2021 , 4 (7) , 6956-6963 . |
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摘要 ：
Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides. Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species. In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers. We developed a computational tool called LibGlycan, which could process the different formats of glycans. Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software. This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively. Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.
关键词 ：
Byonic Byonic Glycan library Glycan library Glycoproteomics Glycoproteomics GlyTouCan GlyTouCan NanoLC-MS/MS NanoLC-MS/MS Rice roots Rice roots
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| GB/T 7714 | Meng, Xianbin , Li, Lijie , Wang, Xiayan . An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses [J]. | TALANTA , 2021 , 234 . |
| MLA | Meng, Xianbin et al. "An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses" . | TALANTA 234 (2021) . |
| APA | Meng, Xianbin , Li, Lijie , Wang, Xiayan . An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses . | TALANTA , 2021 , 234 . |
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摘要 ：
Bone marrow stromal cell antigen 2 (BST-2), also known as CD317 or tetherin, has been identified as a host restriction factor that suppresses the release of enveloped viruses from host cells by physically tethering viral particles to the cell surface; however, this host defense can be subverted by multiple viruses. For example, human immunodeficiency virus (HIV)-1 encodes a specific accessory protein, viral protein U (Vpu), to counteract BST-2 by binding to it and directing its lysosomal degradation. Thus, blocking the interaction between Vpu and BST-2 will provide a promising strategy for anti-HIV therapy. Here, we report a NanoLuc Binary Technology (NanoBiT)-based high-throughput screening assay to detect inhibitors that disrupt the Vpu-BST-2 interaction. Out of more than 1000 compounds screened, four inhibitors were identified with strong activity at nontoxic concentrations. In subsequent cell-based BST-2 degradation assays, inhibitor Y-39983 HCl restored the cell-surface and total cellular level of BST-2 in the presence of Vpu. Furthermore, the Vpu-mediated enhancement of pesudotyped viral particle production was inhibited by Y-39983 HCl. Our findings indicate that our newly developed assay can be used for the discovery of potential antiviral molecules with novel mechanisms of action.
关键词 ：
BST-2 BST-2 high-throughput screening assay high-throughput screening assay HIV-1 HIV-1 NanoLuc Binary Technology NanoLuc Binary Technology Vpu Vpu
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| GB/T 7714 | Li, Boye , Dong, Xiaoxiao , Zhang, Wenmei et al. High-Throughput NanoBiT-Based Screening for Inhibitors of HIV-1 Vpu and Host BST-2 Protein Interaction [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2021 , 22 (17) . |
| MLA | Li, Boye et al. "High-Throughput NanoBiT-Based Screening for Inhibitors of HIV-1 Vpu and Host BST-2 Protein Interaction" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 . 17 (2021) . |
| APA | Li, Boye , Dong, Xiaoxiao , Zhang, Wenmei , Chen, Tian , Yu, Boyang , Zhao, Wenyue et al. High-Throughput NanoBiT-Based Screening for Inhibitors of HIV-1 Vpu and Host BST-2 Protein Interaction . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2021 , 22 (17) . |
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摘要 ：
Three dimensional (3D) DNA walkers hold great potential in serving as an ideal candidate for signal transduction and amplification in bio-assays. However, the autonomous operation of 3D DNA walkers inside living cells is still few and far between, which could be attributed to the lack of suitable driving forces and moderate efficiency in terms of the cellular uptake of such complex 3D DNA components. Herein, a newly updated autonomously operated and highly integrated 3D DNA walker on Au nanoparticles (Au NPs)/zeolitic imidazolate framework-8 (ZIF-8) was activated in a tumor microenviroment and its signal amplified assay capability in living cells was demonstrated using miRNA as a sensing model biomolecule. Specifically, we assembled a 3D DNA motor, including Zn2+-dependent DNAzyme and substrates on the AuNPs grafted on ZIF-8. After being delivered into a living cell, ZIF-8 was efficiently degraded in the tumor microenvironment (low pH value), locally releasing the Zn2+ and DNA motor. Then, a self-sufficient DNA motor autonomously performed the bio-analytical task of imaging miRNA-10b, with a low detection limit of 34 pM. Also, such self-sufficient 3D walkers allowed real-time imaging of MDA-MB-231 cells by intracellular operation. This method demonstrates the self-sufficient 3D DNA motor's bioanalytical application in living cells which may inspire various other biological applications including gene delivery, therapy, etc.
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| GB/T 7714 | Hu, Hui , Zhou, Fu , Wang, Baojuan et al. Autonomous operation of 3D DNA walkers in living cells for microRNA imaging [J]. | NANOSCALE , 2021 , 13 (3) : 1863-1868 . |
| MLA | Hu, Hui et al. "Autonomous operation of 3D DNA walkers in living cells for microRNA imaging" . | NANOSCALE 13 . 3 (2021) : 1863-1868 . |
| APA | Hu, Hui , Zhou, Fu , Wang, Baojuan , Chang, Xin , Dai, Tianyue , Tian, Ruifen et al. Autonomous operation of 3D DNA walkers in living cells for microRNA imaging . | NANOSCALE , 2021 , 13 (3) , 1863-1868 . |
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摘要 ：
An effective and precise electrochemiluminescence resonance energy transfer (ECL-RET), including the efficient regulation over the proximity of a donor and an acceptor and the reliable stimuli responsive as well as the avoidance of undesirable probes leakage, etc., is significant for the development of an accurate and sensitive ECL detection method; yet, the current literature in documentation involves only a limited range of such ECL-RET systems. Herein, we propose an ECL-RET strategy with dually quenched ultralow background signals and a dual-stimuli responsive, accurate signal output for the ultrasensitive and reliable detection of anatoxin-a (ATX-a). The dual quenching is accomplished by an integrated ECL-RET probe of metal organic frameworks (MOFs) encapsulated into Ru(bpy)(3)(2+) (Ru-MOF) (donor) coated with silver nanoparticles (AgNPs) shell (acceptor 1) and close proximity with DNA-ferrocene (Fc) (acceptor 2). Multistimuli responsive DNAzyme facilitated the accurate signal switch by both target ATX-a and hydrogen peroxide (H2O2). Because of the specific recognition of the aptamer toward ATX-a, an intricate design of the DNA sequence enabled the exposure of the Ag+-dependent DNAzyme sequence and H2O2 in situ generated Ag+ triggering a catalytic cleavage reaction to freely release the two ECL-RET energy acceptors, thus switching the ECL signal significantly and achieving ultrasensitive detection. It is noteworthy that AgNPs are key in this ECL-RET strategy, serving both as the gate-keepers for avoiding ECL probes leakage and also the ECL energy acceptors, and mostly importantly serving as the redox substrate for the subsequent DNAzyme catalytic signal switch. The proposed ECL-RET aptasensor for ATX-a detection displayed splendid monitoring performance with a quite low detection limit of 0.00034 mg mL(-1). This sensor not only led to the development of a dual-quenching ECL-RET system but also provided meaningful multistimuli responsive ECL biosensing platform construction, which shows a promising application prospect in complicated sample analysis.
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| GB/T 7714 | Xia, Mengmeng , Zhou, Fu , Feng, Xiuyun et al. A DNAzyme-Based Dual-Stimuli Responsive Electrochemiluminescence Resonance Energy Transfer Platform for Ultrasensitive Anatoxin-a Detection [J]. | ANALYTICAL CHEMISTRY , 2021 , 93 (32) : 11284-11290 . |
| MLA | Xia, Mengmeng et al. "A DNAzyme-Based Dual-Stimuli Responsive Electrochemiluminescence Resonance Energy Transfer Platform for Ultrasensitive Anatoxin-a Detection" . | ANALYTICAL CHEMISTRY 93 . 32 (2021) : 11284-11290 . |
| APA | Xia, Mengmeng , Zhou, Fu , Feng, Xiuyun , Sun, Jiahui , Wang, Li , Li, Na et al. A DNAzyme-Based Dual-Stimuli Responsive Electrochemiluminescence Resonance Energy Transfer Platform for Ultrasensitive Anatoxin-a Detection . | ANALYTICAL CHEMISTRY , 2021 , 93 (32) , 11284-11290 . |
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摘要 ：
Fabrication of economic and high-performance electrodes for electrocatalytic oxygen evolution reaction (OER) accounts for a crucial issue associated with developing powerful and practical water splitting systems. In this work, free-standing Ni/Ni-M (M = Fe, Co, Mo) bimetallic oxides core/shell nanorod arrays (Ni/Ni-M NRAs) were prepared through electroless deposition of transition metal species on black nickel sheet (nickel nanorod arrays (Ni NRAs)) followed by electrochemical oxidation. All three types of Ni/NiM NRAs demonstrated enhanced electrocatalytic activity toward oxygen evolution reactions (OER). Especially, Ni/Ni-Fe NRAs electrode exhibit small onset potential of 1.535 V at current density of 10 mA.cm(-2). In contrast, the OER durability of these three samples was distinct. At 500 mV constant overpotential, the current density loss in OER of Ni/Ni-Fe NRAs was merely 13.5% for a period of 20000 s; but Ni/Ni-Mo and Ni/Ni-Co NRAs had almost disappeared catalytic activity under the identical conditions. According to many reports, the results were different for the superior OER stability of Ni-based bimetallic catalysts. Electrochemical analysis revealed that the NRAs structure dramatically improves charge transfer efficiency and electrochemically active surface area (ECSA). The present study might provide a new insight to design and fabricate more practical and high-performance Ni-based electrodes for OER. (C) 2021 Elsevier Ltd. All rights reserved.
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| GB/T 7714 | Yan, Yong , Liu, Haocen , Liu, Chunyue et al. Efficient preparation of Ni-M (M = Fe, Co, Mo) bimetallic oxides layer on Ni nanorod arrays for electrocatalytic oxygen evolution [J]. | APPLIED MATERIALS TODAY , 2021 , 25 . |
| MLA | Yan, Yong et al. "Efficient preparation of Ni-M (M = Fe, Co, Mo) bimetallic oxides layer on Ni nanorod arrays for electrocatalytic oxygen evolution" . | APPLIED MATERIALS TODAY 25 (2021) . |
| APA | Yan, Yong , Liu, Haocen , Liu, Chunyue , Zhao, Yuguo , Liu, Shuzhen , Wang, Dong et al. Efficient preparation of Ni-M (M = Fe, Co, Mo) bimetallic oxides layer on Ni nanorod arrays for electrocatalytic oxygen evolution . | APPLIED MATERIALS TODAY , 2021 , 25 . |
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摘要 ：
Many diseases are related to abnormal metabolites in cells. It is of great significance to analyze the metabolites for cellular physiological, pathological, and pharmacological research. Metabolite analysis at the single-cell level is extremely important due to cell heterogeneity. Electrospray ionization mass spectrometry (ESI-MS) has become an indispensable method for single-cell metabolite analysis with the following advantages: detection of unlabeled substrates, in situ and real-time in vivo analyses, broad-spectrum analysis, and excellent qualitative ability. In this review, we discuss published articles in the field of single-cell metabolite analysis by ESI-MS over recent years. ESI-MS methods are classified and reviewed according to the methods of obtaining single-cell samples. The achievements with respect to different approaches to sensitivity, throughput, coverage, and system stability are reviewed, and the methods with great breakthroughs are summarized and discussed. Finally, the development trend of single-cell metabolite analysis by ESI-MS is prospected. (C) 2021 Elsevier B.V. All rights reserved.
关键词 ：
Analytical methods Analytical methods Cellular heterogeneity Cellular heterogeneity Electrospray ionization Electrospray ionization Mass spectrometry Mass spectrometry Metabolite analysis Metabolite analysis Single cell Single cell
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| GB/T 7714 | Zhu, Guizhen , Shao, Yunlong , Liu, Yuanxing et al. Single-cell metabolite analysis by electrospray ionization mass spectrometry [J]. | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2021 , 143 . |
| MLA | Zhu, Guizhen et al. "Single-cell metabolite analysis by electrospray ionization mass spectrometry" . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY 143 (2021) . |
| APA | Zhu, Guizhen , Shao, Yunlong , Liu, Yuanxing , Pei, Tong , Li, Lijie , Zhang, Dongtang et al. Single-cell metabolite analysis by electrospray ionization mass spectrometry . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2021 , 143 . |
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摘要 ：
The analysis of single living cells, including intracellular delivery and extraction, is essential for monitoring their dynamic biochemical processes and exploring intracellular heterogeneity. However, owing to the 2D view in bright-field microscopy and optical distortions caused by the cell shape and the variation in the refractive index both inside and around the cells, achieving spatially undistorted imaging for high-precision manipulation within a cell is challenging. Here, an accurate and visual system is developed for single-cell spatial manipulation by correcting the aberration for simultaneous bright-field triple-view imaging. Stereo information from the triple view enables higher spatial resolution that facilitates the precise manipulation of single cells. In the bright field, we resolved the spatial locations of subcellular structures of a single cell suspended in a medium and measured the random spatial rotation angle of the cell with a precision of +/- 5 degrees. Furthermore, we demonstrated the visual manipulation of a probe to an arbitrary spatial point of a cell with an accuracy of <1 pixel. This novel system is more accurate and less destructive for subcellular content extraction and drug delivery.
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| GB/T 7714 | Zhang, Qi , Shao, Yunlong , Li, Boye et al. Visually precise, low-damage, single-cell spatial manipulation with single-pixel resolution [J]. | CHEMICAL SCIENCE , 2021 , 12 (11) : 4111-4118 . |
| MLA | Zhang, Qi et al. "Visually precise, low-damage, single-cell spatial manipulation with single-pixel resolution" . | CHEMICAL SCIENCE 12 . 11 (2021) : 4111-4118 . |
| APA | Zhang, Qi , Shao, Yunlong , Li, Boye , Wu, Yuanyuan , Dong, Jingying , Zhang, Dongtang et al. Visually precise, low-damage, single-cell spatial manipulation with single-pixel resolution . | CHEMICAL SCIENCE , 2021 , 12 (11) , 4111-4118 . |
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摘要 ：
Hydrodynamic chromatography (HDC) is a technique originally developed for separating particles. We have recently extended it to DNA fragment sizing and quantitation. In this review, we focus on this extension. After we briefly introduce the history of HDC, we present the evolution of open tubular HDC for DNA fragment sizing. We cover both the theoretical aspect and the experimental implementation of this technique. We describe various approaches to execute the separation, discuss its representative applications and provide a future perspective of this technique in the conclusion section of this review.
关键词 ：
DNA fragment Quantitation DNA fragment Quantitation DNA fragment Sizing DNA fragment Sizing Free solution separation Free solution separation Hydrodynamic chromatography Hydrodynamic chromatography Open tubular column Open tubular column
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| GB/T 7714 | Wang, Yanan , Zhou, Yingyan , Zhang, Dongtang et al. Extension of hydrodynamic chromatography to DNA fragment sizing and quantitation [J]. | HELIYON , 2021 , 7 (9) . |
| MLA | Wang, Yanan et al. "Extension of hydrodynamic chromatography to DNA fragment sizing and quantitation" . | HELIYON 7 . 9 (2021) . |
| APA | Wang, Yanan , Zhou, Yingyan , Zhang, Dongtang , Wang, Xiayan , Liu, Shaorong . Extension of hydrodynamic chromatography to DNA fragment sizing and quantitation . | HELIYON , 2021 , 7 (9) . |
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