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摘要 ：
The nitrogen removal characteristics, physicochemical properties, and microbial community composition of four different anaerobic ammonium oxidation (anammox) sludge morphologies were investigated. The morphologies considered in this study, namely suspended sludge (Rs), biofilm (Rm), granular sludge (Rg), and encapsulated biomass (Re), were prepared from floc sludge. The results show that Re exhibited the maximum anammox activity, followed by Rg, Rm, and Rs. Additionally, the anammox contribution rate was higher in Rg and Re. The higher extracellular polymer content in Rg promoted sludge accumulation, and tryptophan was observed in Rm and Rg, which was replaced by humic acids in Rs. Re showed the largest specific surface area, hydrophobicity and strength, and its good structure ensured enrichment of anammox bacteria (AnAOB). In terms of the microbial community, the functional bacterium Candidatus Kuenenia accounted for the highest proportion in Rm (39.27%), but the presence of both anaerobic and aerobic regions led to increased community complexity with more nitrifying bacteria. In contrast, Rg and Re had a more specific microbial community. In addition, denitrifying bacteria tended to grow in Rs, while nitrifying bacteria were retained in Rm. The AnAOB were more likely to be enriched in sludge aggregates (both Rm and Rg) and carriers (Re). Through correlation analysis, the potential relationship involving bacterial flora evolution of each sample was clarified. Finally, the structural models of different morphologies of sludge were proposed. This study deepens the understanding of various anammox sludge morphologies as well as provides useful information for the cultivation of AnAOB and further application of anammox.
关键词 ：
Anammox Anammox Biofilm Biofilm Flora structure Flora structure Granular sludge Granular sludge Immobilized filler Immobilized filler Sludge morphology Sludge morphology
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| GB/T 7714 | Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community [J]. | CHEMOSPHERE , 2022 , 288 . |
| MLA | Wang, XiaoTong et al. "Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community" . | CHEMOSPHERE 288 (2022) . |
| APA | Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community . | CHEMOSPHERE , 2022 , 288 . |
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摘要 ：
The immobilized carrier was prepared with complete anaerobic ammonia oxidation granular sludge (AnGS) and crushed AnGS, respectively. We evaluated the effects of granular form and continuous temperature changes on nitrogen removal by immobilized anaerobic ammonium oxidation (anammox) filler. The results showed that the rate of nitrogen removal of crushed and encapsulated AnGS was 20% higher than that of direct encapsulated AnGS. However, the latter had higher thresholds of tolerance to Fe2+ and Cu2+. In addition, the immobilization reduced the activation energy of anammox. Above 12.5 degrees C, the immobilized filler was efficient at removing nitrogen removal through the dual adjustment of temperature-hydraulic retention time. From 12.5 degrees C to 23 degrees C, the temperature had a greater influence on the nitrogen removal effect than the HRT. In contrast, HRT had a dominant influence from 23 degrees C to 32 degrees C. Anammox activity was severely inhibited below 12.5 degrees C. High-throughput sequencing analysis showed that the community structure migrated with the changes in temperature. The anammox functional bacteria Candidatus Kuenenia (18.31-39.73%) were the dominant genus at medium and high temperatures, and it was replaced by Chryseobacterium (24.19%) at 8.5 degrees C. In addition, an RDA analysis showed that Candidatus Brocadia was more adaptable to low temperatures than Candidatus Kuenenia. In addition, Bellilinea was more sensitive to temperature than Candidatus Kuenenia. Thus, the temperature could be appropriately lowered to avoid overbreeding. The results of this study optimized the operation of an anammox immobilized system and promote its further application. (C) 2021 Elsevier B.V. All rights reserved.
关键词 ：
Anammox Anammox Temperature Temperature AnGS form AnGS form Metal ions Metal ions Microflora structure Microflora structure Immobilized filler Immobilized filler
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| GB/T 7714 | Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
| MLA | Wang, XiaoTong et al. "Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis" . | SCIENCE OF THE TOTAL ENVIRONMENT 803 (2022) . |
| APA | Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
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摘要 ：
The treatment performance of anaerobic ammonia oxidation (anammox) immobilized filler on different proportions of domestic wastewater was evaluated. The results showed that, in comparison to synthetic wastewater, 50% domestic wastewater promoted the anammox reaction of immobilized filler, while 100% domestic wastewater had no significant effect on the anammox activity of immobilized filler but the total nitrogen removal efficiency (TNRE) was improved through enhanced denitrification. The TNRE of the immobilized filler was 82.5%, which was significantly higher than that of AnGS (69.7%), and its average anammox contribution rate was more than 90%. This was because the encapsulated anammox biomass could better maintain competitive advantages and coordinate the symbiotic relationship with denitrifying bacteria. Moreover, lower NH4+-N concentration resulted in greater influence of C/N ratio on anammox performance than COD concentration, while the opposite was true at high NH4+-N concentration. This study verified that anammox immobilized filler is effective for mainstream applications.
关键词 ：
Microbial composition Microbial composition Domestic wastewater Domestic wastewater Anammox Anammox Immobilized filler Immobilized filler C/N ratio C/N ratio
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| GB/T 7714 | Wang, Xiao Tong , Yang, Hong , Zhou, YaKun et al. Performance and mechanism analysis of gel immobilized anammox bacteria in treating different proportions of domestic wastewater: a valid alternative to granular sludge [J]. | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
| MLA | Wang, Xiao Tong et al. "Performance and mechanism analysis of gel immobilized anammox bacteria in treating different proportions of domestic wastewater: a valid alternative to granular sludge" . | BIORESOURCE TECHNOLOGY 347 (2022) . |
| APA | Wang, Xiao Tong , Yang, Hong , Zhou, YaKun , Liu, XuYan . Performance and mechanism analysis of gel immobilized anammox bacteria in treating different proportions of domestic wastewater: a valid alternative to granular sludge . | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
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摘要 ：
采用彗星纤维填料挂膜方式在连续流下流式厌氧反应器(DAR)+上流式厌氧污泥反应器(UASB)中对硫酸盐还原菌(Sulfate-Reducing Bacteria,SRB)进行富集培养,以高通量结果分析SRB群落中种群变化结果,最后将SRB投入酸性矿山废水(Acid Mine Drainage,AMD)中考察富集培养后SRB对AMD的适应性。结果表明:阶段式提升硫酸盐浓度能够实现SRB的快速稳定增长,且有利于使SRB抑制其他种群成为优势菌群;历时35 d,硫酸盐去除率和去除效率分别稳定在83%和396 mg/(L·h)以上;富集后的SRB功能菌占比由58.68%升至94.08%,不完全氧化SRB...
关键词 ：
酸性矿山废水适应性 酸性矿山废水适应性 硫酸盐还原菌 硫酸盐还原菌 高通量测序 高通量测序 富集培养 富集培养 优势菌群 优势菌群
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| GB/T 7714 | 胡鑫 , 杨宏 , 方小月 et al. 硫酸盐还原菌的富集及其对酸性矿山废水的适应性研究 [C] //中国环境科学学会2021年科学技术年会——环境工程技术创新与应用分会场论文集（四） . 2021 . |
| MLA | 胡鑫 et al. "硫酸盐还原菌的富集及其对酸性矿山废水的适应性研究" 中国环境科学学会2021年科学技术年会——环境工程技术创新与应用分会场论文集（四） . (2021) . |
| APA | 胡鑫 , 杨宏 , 方小月 , 刘旭妍 , 刘宗跃 . 硫酸盐还原菌的富集及其对酸性矿山废水的适应性研究 中国环境科学学会2021年科学技术年会——环境工程技术创新与应用分会场论文集（四） . (2021) . |
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摘要 ：
我国南海处于极端热湿气候区,为顺应岛礁开发建设与可持续发展,建筑应更多地采取被动式策略以降低能耗.虽然南海岛礁常年高温、高湿,但气温日较差与年较差小、风力大,建筑具有潜在的自然通风能力.应用建筑气候学相关原理,以标准有效温度SET作为评价指标与方法,综合温度、湿度、风速等因素,全面评价岛礁的自然通风潜力.选取南海地区6座典型位置的岛礁,采用参数化方法对标准有效温度进行计算分析,得出满足热舒适要求的标准有效温度全年时间占比,并评价其自然通风潜力.同时研究了温度、风速对标准有效温度的影响.
关键词 ：
极端热湿气候区 极端热湿气候区 热舒适 热舒适 标准有效温度 标准有效温度 自然通风潜力 自然通风潜力
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| GB/T 7714 | 李宝龙 , 杨红 , 谢静超 . 极端热湿气候区建筑自然通风潜力评价 [J]. | 建筑节能 , 2021 , 49 (1) : 42-46 . |
| MLA | 李宝龙 et al. "极端热湿气候区建筑自然通风潜力评价" . | 建筑节能 49 . 1 (2021) : 42-46 . |
| APA | 李宝龙 , 杨红 , 谢静超 . 极端热湿气候区建筑自然通风潜力评价 . | 建筑节能 , 2021 , 49 (1) , 42-46 . |
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摘要 ：
基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析
关键词 ：
厌氧氨氧化(ANAMMOX) 厌氧氨氧化(ANAMMOX) 基因功能预测 基因功能预测 微生物群落结构 微生物群落结构 比活性 比活性 粒径 粒径
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| GB/T 7714 | 王晓曈 , 杨宏 , 环境科学 . 基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 [J]. | 王晓曈 , 2021 , 42 (4) : 1930-1938 . |
| MLA | 王晓曈 et al. "基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析" . | 王晓曈 42 . 4 (2021) : 1930-1938 . |
| APA | 王晓曈 , 杨宏 , 环境科学 . 基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 . | 王晓曈 , 2021 , 42 (4) , 1930-1938 . |
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摘要 ：
为明确基于粒径分化的厌氧氨氧化(ANAMMOX)污泥特性与菌群演替规律,研究了高氨氮生物滤池反应体系中ANAMMOX絮体污泥形成颗粒过程中的性能变化和微生物群落结构.结果表明,粒径增加显著提高了厌氧氨氧化颗粒污泥(AnGS)比活性(SAA)和耐受性,R4(> 4.75 mm)的SAA最高为426.8 mg·(g·d)~(-1),但也给传质带来不利影响.高通量测序结果显示,菌属间动态变化普遍存在.<4.75 mm时,粒径增加强化了菌群结构稳定性,絮体中含量较多的氨氧化细菌(AOB)被淘汰,脱氮比例逐渐稳定.R3(2.8～4.75 mm)具有最专一的菌群组成,功能菌Candidatus Kuene...
关键词 ：
厌氧氨氧化(ANAMMOX) 厌氧氨氧化(ANAMMOX) 基因功能预测 基因功能预测 微生物群落结构 微生物群落结构 比活性 比活性 粒径 粒径
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| GB/T 7714 | 王晓曈 , 杨宏 . 基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 [J]. | 环境科学 , 2021 , 42 (04) : 1930-1938 . |
| MLA | 王晓曈 et al. "基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析" . | 环境科学 42 . 04 (2021) : 1930-1938 . |
| APA | 王晓曈 , 杨宏 . 基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 . | 环境科学 , 2021 , 42 (04) , 1930-1938 . |
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摘要 ：
In order to clarify the characteristics of anaerobic ammonia oxidizing (ANAMMOX) sludge and the succession rule of bacteria based on particle size differentiation, the performance change and microbial community structure of ANAMMOX floc sludge during the formation of particles in the reaction system of a high ammonia-nitrogen biofilter were studied. The results indicated that the specific activity (SAA) and tolerance of the ANAMMOX granular sludge (AnGS) were significantly improved by increasing the particle size, and the SAA of R4(>4.75 mm) was up to 426.8 mg·(g·d)-1, but it also had adverse effects on mass transfer. The results of the high-throughput sequencing showed that dynamic changes between bacterial genera were common. When the particle size was less than 4.75 mm, the increase in particle size strengthened the stability of the bacterial flora, the ammonia oxidizing bacteria (AOB) with more flocs were eliminated, and the nitrogen removal ratio gradually stabilized. R3 (2.8-4.75 mm) exhibited the most specific flora composition, and the functional bacteria Candidatus Kuenenia accounted for 52.7%, while the R4 community complexity increased. Furthermore, the proportion of functional bacteria decreased, and the abundance of heterotrophic bacteria increased, which negatively affected the particle structure. In addition, the R3 microorganism has the best gene function expression level, which is significantly better than small particles in gene replication repair and energy conversion. Finally, the evolution of AnGS was analyzed through the OTU matrix between the samples. These results have some guiding significance for the optimization of the AnGS system and will be helpful for the application of the ANAMMOX process.
关键词 ：
anaerobic ammonium oxidation (ANAMMOX) anaerobic ammonium oxidation (ANAMMOX) gene function prediction gene function prediction microbial community structure microbial community structure particle size particle size specific activity specific activity
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| GB/T 7714 | Wang Xiao-Tong , Yang Hong . [Analysis of Performance and Microbial Diversity of ANAMMOX Sludge Based on Particle Size Differentiation]. [J]. | Huanjing kexue , 2021 , 42 (4) : 1930-1938 . |
| MLA | Wang Xiao-Tong et al. "[Analysis of Performance and Microbial Diversity of ANAMMOX Sludge Based on Particle Size Differentiation]." . | Huanjing kexue 42 . 4 (2021) : 1930-1938 . |
| APA | Wang Xiao-Tong , Yang Hong . [Analysis of Performance and Microbial Diversity of ANAMMOX Sludge Based on Particle Size Differentiation]. . | Huanjing kexue , 2021 , 42 (4) , 1930-1938 . |
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摘要 ：
This study focused on the problem that simultaneous nitrogen and phosphorus removal cannot be achieved in current urban sewage treatment systems. To address this, a pilot system was developed to first use immobilized biologically active fillers to remove nitrogen, and then use activated sludge to independently remove phosphorus. The goal was to maximize the effectiveness of biological phosphorus removal. This study mainly aimed at analyzing the post-independent phosphorus removal characteristics and mechanisms; the composition of the phosphorus removing bacteria; and the contribution rate of the system. The effluent PO43--P of the system remained stable at 0.15-0.2 mg L-1, with the phosphorus removal anaerobic tank maintaining a "micro-release" state. When the hydraulic retention time (HRT) of the aerobic phosphorus removal tank was reduced to 1 h, the contribution rate of the aerobic phosphorus removal bacteria decreased from 28.42% to 16.68%; the contribution rate of the denitrifying phosphorus removal bacteria increased from 72.58% to 83.32%; and the abundance of the dominant bacteria Dechloromonas and Thaurea significantly increased. The phosphorus removal system provides a new approach for deep phosphorus removal in main municipal wastewater systems. (C) 2021 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
关键词 ：
Immobilized filler Immobilized filler Microbial community structure Microbial community structure Micro-release state Micro-release state Phosphorus removal contribution Phosphorus removal contribution Post-independent biological phosphorus removal Post-independent biological phosphorus removal
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| GB/T 7714 | Liu, Xuyan , Yang, Hong , Wang, Shaolun et al. Study on the effectiveness of an independent biological phosphorus removal system based on immobilized biological fillers nitrogen removal system in municipal wastewater [J]. | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION , 2021 , 156 : 17-28 . |
| MLA | Liu, Xuyan et al. "Study on the effectiveness of an independent biological phosphorus removal system based on immobilized biological fillers nitrogen removal system in municipal wastewater" . | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION 156 (2021) : 17-28 . |
| APA | Liu, Xuyan , Yang, Hong , Wang, Shaolun , Fang, Xiaoyue , Wang, Xiaotong , Bai, Yongsheng et al. Study on the effectiveness of an independent biological phosphorus removal system based on immobilized biological fillers nitrogen removal system in municipal wastewater . | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION , 2021 , 156 , 17-28 . |
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摘要 ：
The characteristics of anammox granular sludge (AnGS) based on color differentiation, and the regulation mechanism of immobilized fillers in the system were investigated. The results showed that biomass content, EPS and activity of red AnGS (R1) were higher than those of brown AnGS (R2). Moreover, R1 showed nitrification, while R2 showed denitrification. Filamentous bacteria constituted the granule skeleton of R1, while R2 mainly constituted inorganic nucleation and granulation. Additionally, immobilization improved the contribution rate of Anammox, and involved different regulatory mechanisms. High-throughput sequencing analysis showed that R1 encapsulation biomass eliminated miscellaneous bacteria and established specific flora, while mixed encapsulated biomass of R1 and R2 re-formed a functional bacterial network, which strengthened interspecies cooperation. The R2 encapsulated biomass and AnAOB copy numbers were inferior and the interspecific cooperation was weak, resulting in an unsatisfactory nitrogen removal performance. These results can strengthen the understanding and optimization of AnGS and its immobilization system.
关键词 ：
Anammox bacteria Anammox bacteria Granular sludge color Granular sludge color Immobilized filler Immobilized filler Microbial community structure Microbial community structure Nitrogen removal Nitrogen removal
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| GB/T 7714 | Wang XiaoTong , Yang Hong , Su Yang et al. Characteristics of anammox granular sludge using color differentiation, and nitrogen removal performance of its immobilized fillers based on microbial succession. [J]. | Bioresource technology , 2021 , 333 : 125188 . |
| MLA | Wang XiaoTong et al. "Characteristics of anammox granular sludge using color differentiation, and nitrogen removal performance of its immobilized fillers based on microbial succession." . | Bioresource technology 333 (2021) : 125188 . |
| APA | Wang XiaoTong , Yang Hong , Su Yang , Liu XuYan , Wang JiaWei . Characteristics of anammox granular sludge using color differentiation, and nitrogen removal performance of its immobilized fillers based on microbial succession. . | Bioresource technology , 2021 , 333 , 125188 . |
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