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摘要 ：
The nitrogen removal characteristics, physicochemical properties, and microbial community composition of four different anaerobic ammonium oxidation (anammox) sludge morphologies were investigated. The morphologies considered in this study, namely suspended sludge (Rs), biofilm (Rm), granular sludge (Rg), and encapsulated biomass (Re), were prepared from floc sludge. The results show that Re exhibited the maximum anammox activity, followed by Rg, Rm, and Rs. Additionally, the anammox contribution rate was higher in Rg and Re. The higher extracellular polymer content in Rg promoted sludge accumulation, and tryptophan was observed in Rm and Rg, which was replaced by humic acids in Rs. Re showed the largest specific surface area, hydrophobicity and strength, and its good structure ensured enrichment of anammox bacteria (AnAOB). In terms of the microbial community, the functional bacterium Candidatus Kuenenia accounted for the highest proportion in Rm (39.27%), but the presence of both anaerobic and aerobic regions led to increased community complexity with more nitrifying bacteria. In contrast, Rg and Re had a more specific microbial community. In addition, denitrifying bacteria tended to grow in Rs, while nitrifying bacteria were retained in Rm. The AnAOB were more likely to be enriched in sludge aggregates (both Rm and Rg) and carriers (Re). Through correlation analysis, the potential relationship involving bacterial flora evolution of each sample was clarified. Finally, the structural models of different morphologies of sludge were proposed. This study deepens the understanding of various anammox sludge morphologies as well as provides useful information for the cultivation of AnAOB and further application of anammox.
关键词 ：
Anammox Anammox Biofilm Biofilm Flora structure Flora structure Granular sludge Granular sludge Immobilized filler Immobilized filler Sludge morphology Sludge morphology
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| GB/T 7714 | Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community [J]. | CHEMOSPHERE , 2022 , 288 . |
| MLA | Wang, XiaoTong et al. "Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community" . | CHEMOSPHERE 288 (2022) . |
| APA | Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community . | CHEMOSPHERE , 2022 , 288 . |
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摘要 ：
The immobilized carrier was prepared with complete anaerobic ammonia oxidation granular sludge (AnGS) and crushed AnGS, respectively. We evaluated the effects of granular form and continuous temperature changes on nitrogen removal by immobilized anaerobic ammonium oxidation (anammox) filler. The results showed that the rate of nitrogen removal of crushed and encapsulated AnGS was 20% higher than that of direct encapsulated AnGS. However, the latter had higher thresholds of tolerance to Fe2+ and Cu2+. In addition, the immobilization reduced the activation energy of anammox. Above 12.5 degrees C, the immobilized filler was efficient at removing nitrogen removal through the dual adjustment of temperature-hydraulic retention time. From 12.5 degrees C to 23 degrees C, the temperature had a greater influence on the nitrogen removal effect than the HRT. In contrast, HRT had a dominant influence from 23 degrees C to 32 degrees C. Anammox activity was severely inhibited below 12.5 degrees C. High-throughput sequencing analysis showed that the community structure migrated with the changes in temperature. The anammox functional bacteria Candidatus Kuenenia (18.31-39.73%) were the dominant genus at medium and high temperatures, and it was replaced by Chryseobacterium (24.19%) at 8.5 degrees C. In addition, an RDA analysis showed that Candidatus Brocadia was more adaptable to low temperatures than Candidatus Kuenenia. In addition, Bellilinea was more sensitive to temperature than Candidatus Kuenenia. Thus, the temperature could be appropriately lowered to avoid overbreeding. The results of this study optimized the operation of an anammox immobilized system and promote its further application. (C) 2021 Elsevier B.V. All rights reserved.
关键词 ：
Anammox Anammox AnGS form AnGS form Immobilized filler Immobilized filler Metal ions Metal ions Microflora structure Microflora structure Temperature Temperature
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| GB/T 7714 | Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
| MLA | Wang, XiaoTong et al. "Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis" . | SCIENCE OF THE TOTAL ENVIRONMENT 803 (2022) . |
| APA | Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
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摘要 ：
采用彗星纤维填料挂膜方式在连续流下流式厌氧反应器(DAR)+上流式厌氧污泥反应器(UASB)中对硫酸盐还原菌(Sulfate-Reducing Bacteria,SRB)进行富集培养,以高通量结果分析SRB群落中种群变化结果,最后将SRB投入酸性矿山废水(Acid Mine Drainage,AMD)中考察富集培养后SRB对AMD的适应性。结果表明:阶段式提升硫酸盐浓度能够实现SRB的快速稳定增长,且有利于使SRB抑制其他种群成为优势菌群;历时35 d,硫酸盐去除率和去除效率分别稳定在83%和396 mg/(L·h)以上;富集后的SRB功能菌占比由58.68%升至94.08%,不完全氧化SRB...
关键词 ：
酸性矿山废水适应性 酸性矿山废水适应性 硫酸盐还原菌 硫酸盐还原菌 高通量测序 高通量测序 富集培养 富集培养 优势菌群 优势菌群
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| GB/T 7714 | 胡鑫 , 杨宏 , 方小月 et al. 硫酸盐还原菌的富集及其对酸性矿山废水的适应性研究 [C] //中国环境科学学会2021年科学技术年会——环境工程技术创新与应用分会场论文集（四） . 2021 . |
| MLA | 胡鑫 et al. "硫酸盐还原菌的富集及其对酸性矿山废水的适应性研究" 中国环境科学学会2021年科学技术年会——环境工程技术创新与应用分会场论文集（四） . (2021) . |
| APA | 胡鑫 , 杨宏 , 方小月 , 刘旭妍 , 刘宗跃 . 硫酸盐还原菌的富集及其对酸性矿山废水的适应性研究 中国环境科学学会2021年科学技术年会——环境工程技术创新与应用分会场论文集（四） . (2021) . |
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摘要 ：
我国南海处于极端热湿气候区,为顺应岛礁开发建设与可持续发展,建筑应更多地采取被动式策略以降低能耗.虽然南海岛礁常年高温、高湿,但气温日较差与年较差小、风力大,建筑具有潜在的自然通风能力.应用建筑气候学相关原理,以标准有效温度SET作为评价指标与方法,综合温度、湿度、风速等因素,全面评价岛礁的自然通风潜力.选取南海地区6座典型位置的岛礁,采用参数化方法对标准有效温度进行计算分析,得出满足热舒适要求的标准有效温度全年时间占比,并评价其自然通风潜力.同时研究了温度、风速对标准有效温度的影响.
关键词 ：
极端热湿气候区 极端热湿气候区 热舒适 热舒适 标准有效温度 标准有效温度 自然通风潜力 自然通风潜力
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| GB/T 7714 | 李宝龙 , 杨红 , 谢静超 . 极端热湿气候区建筑自然通风潜力评价 [J]. | 建筑节能 , 2021 , 49 (1) : 42-46 . |
| MLA | 李宝龙 et al. "极端热湿气候区建筑自然通风潜力评价" . | 建筑节能 49 . 1 (2021) : 42-46 . |
| APA | 李宝龙 , 杨红 , 谢静超 . 极端热湿气候区建筑自然通风潜力评价 . | 建筑节能 , 2021 , 49 (1) , 42-46 . |
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摘要 ：
基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析
关键词 ：
厌氧氨氧化(ANAMMOX) 厌氧氨氧化(ANAMMOX) 基因功能预测 基因功能预测 微生物群落结构 微生物群落结构 比活性 比活性 粒径 粒径
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| GB/T 7714 | 王晓曈 , 杨宏 , 环境科学 . 基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 [J]. | 王晓曈 , 2021 , 42 (4) : 1930-1938 . |
| MLA | 王晓曈 et al. "基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析" . | 王晓曈 42 . 4 (2021) : 1930-1938 . |
| APA | 王晓曈 , 杨宏 , 环境科学 . 基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 . | 王晓曈 , 2021 , 42 (4) , 1930-1938 . |
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摘要 ：
为明确基于粒径分化的厌氧氨氧化(ANAMMOX)污泥特性与菌群演替规律,研究了高氨氮生物滤池反应体系中ANAMMOX絮体污泥形成颗粒过程中的性能变化和微生物群落结构.结果表明,粒径增加显著提高了厌氧氨氧化颗粒污泥(AnGS)比活性(SAA)和耐受性,R4(> 4.75 mm)的SAA最高为426.8 mg·(g·d)~(-1),但也给传质带来不利影响.高通量测序结果显示,菌属间动态变化普遍存在.<4.75 mm时,粒径增加强化了菌群结构稳定性,絮体中含量较多的氨氧化细菌(AOB)被淘汰,脱氮比例逐渐稳定.R3(2.8～4.75 mm)具有最专一的菌群组成,功能菌Candidatus Kuene...
关键词 ：
厌氧氨氧化(ANAMMOX) 厌氧氨氧化(ANAMMOX) 基因功能预测 基因功能预测 微生物群落结构 微生物群落结构 比活性 比活性 粒径 粒径
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| GB/T 7714 | 王晓曈 , 杨宏 . 基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 [J]. | 环境科学 , 2021 , 42 (04) : 1930-1938 . |
| MLA | 王晓曈 et al. "基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析" . | 环境科学 42 . 04 (2021) : 1930-1938 . |
| APA | 王晓曈 , 杨宏 . 基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 . | 环境科学 , 2021 , 42 (04) , 1930-1938 . |
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摘要 ：
Two EGSB (expanded granular sludge bed) reactors (R0 and R1) with 2 g-VSS L-1 anammox sludge were established to intensify specific anammox activity (SAA) and biomass growth under limited inoculum, and in response to a hydraulic strategy. During the start-up period, HRT (hydraulic retention time) was set at 12h in R0, while sharply decreased from 12h to 4h in R1. The sludge characteristics, abundance, and community shifts were also investigated to explore their correlations with SAA enhancement. The results showed that after 120 days, SAA of R1 finally reached 1.6 kg-N kg-VSS-1 per day, which was 1.8- times that of R0. The initial shortened HRT in R1 changed the assembly mode of granulation, and unlike the flocculant sludge in R0, the formation and growth of small granules played a more critical role in improving SAA in R1. Also, the tightly bound layer of extracellular polymeric substances promoted a rapid increase in the proportion of particles larger than 0.6 mm in size, which significantly enhanced SAA. QPCR showed the corresponding abundance of anammox bacteria was gradually saturated to 5.72E+10 copies g-SS-1 when the SAA tended to be stable. While the increase in "saturated abundance" particles (>1.0 mm) speeded up the proliferation of anammox bacteria. The corresponding doubling time was shortened from 36.8-10.0 d in R1, which made up for the disadvantage of the initial biomass loss. The high-throughput analysis revealed Candidatus Kuenenia stuttgartiensis was the dominant genus (34.22 %) in R1.
关键词 ：
Abundance Abundance Anammox Anammox Double time Double time Hydraulic strategy Hydraulic strategy Specific anammox activity Specific anammox activity
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| GB/T 7714 | Su, Yang , Yang, Hong , Wang, Xiaotong et al. Successful enhancement and related characteristics of anammox bacterial activity by hydraulic supply strategy under limited inoculum [J]. | JOURNAL OF WATER PROCESS ENGINEERING , 2021 , 41 . |
| MLA | Su, Yang et al. "Successful enhancement and related characteristics of anammox bacterial activity by hydraulic supply strategy under limited inoculum" . | JOURNAL OF WATER PROCESS ENGINEERING 41 (2021) . |
| APA | Su, Yang , Yang, Hong , Wang, Xiaotong , Liu, Xuyan , Wang, Jiawei . Successful enhancement and related characteristics of anammox bacterial activity by hydraulic supply strategy under limited inoculum . | JOURNAL OF WATER PROCESS ENGINEERING , 2021 , 41 . |
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摘要 ：
We established a study on the characteristics of hydrolytic acidification immobilized fillers and sludge in the pretreatment of municipal wastewater. In long-term stable operation, temperature had a greater impact on the difference between the hydrolysis-acidification performance of sludge and the filler. We compared samples at 50 d (25 +/- 1 degrees C), 80 d (18 +/- 1 degrees C), and 120 d (13 +/- 1 degrees C) in the stable operation phase, investigating effluent organic matter composition and microbial community changes. At the lowest temperature (13 +/- 1 degrees C), the effluent of sludge showed residual dissolved organic nitrogen (DON) levels of 2.51-3.24 mg L-1, whereas that of the filler had a low DON level (0.78-0.91 mg L-1). The DON removal effect of the filler was higher than that of sludge; the residual DON was protein-like in both reactors. The dominant bacterial genera differed with temperature. At the low temperature, although the sludge maintained a certain hydrolysis-acidification potential, a relatively high abundance (3.71%) of filamentous bacteria was found, causing the sludge to rise and expand. The abundance of filamentous bacteria in the filler was only 0.14%, which was related to the stable structure of the immobilized filler. (C) 2021 Elsevier B.V. All rights reserved.
关键词 ：
Chemical characteristics Chemical characteristics Hydrolysis-acidification Hydrolysis-acidification Immobilized fillers Immobilized fillers Key microorganisms Key microorganisms Temperature Temperature
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| GB/T 7714 | Liu, Xuyan , Yang, Hong , Fang, Xiaoyue et al. Comparative study on the performance of hydrolytic acidification immobilized filler and sludge in the pretreatment of municipal wastewater [J]. | ENVIRONMENTAL TECHNOLOGY & INNOVATION , 2021 , 24 . |
| MLA | Liu, Xuyan et al. "Comparative study on the performance of hydrolytic acidification immobilized filler and sludge in the pretreatment of municipal wastewater" . | ENVIRONMENTAL TECHNOLOGY & INNOVATION 24 (2021) . |
| APA | Liu, Xuyan , Yang, Hong , Fang, Xiaoyue , Bai, Yongsheng , Su, Bojun , Huang, Hongyan . Comparative study on the performance of hydrolytic acidification immobilized filler and sludge in the pretreatment of municipal wastewater . | ENVIRONMENTAL TECHNOLOGY & INNOVATION , 2021 , 24 . |
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摘要 ：
In order to clarify the characteristics of anaerobic ammonia oxidizing (ANAMMOX) sludge and the succession rule of bacteria based on particle size differentiation, the performance change and microbial community structure of ANAMMOX floc sludge during the formation of particles in the reaction system of a high ammonia-nitrogen biofilter were studied. The results indicated that the specific activity (SAA) and tolerance of the ANAMMOX granular sludge (AnGS) were significantly improved by increasing the particle size, and the SAA of R4(>4.75 mm) was up to 426.8 mg·(g·d)-1, but it also had adverse effects on mass transfer. The results of the high-throughput sequencing showed that dynamic changes between bacterial genera were common. When the particle size was less than 4.75 mm, the increase in particle size strengthened the stability of the bacterial flora, the ammonia oxidizing bacteria (AOB) with more flocs were eliminated, and the nitrogen removal ratio gradually stabilized. R3 (2.8-4.75 mm) exhibited the most specific flora composition, and the functional bacteria Candidatus Kuenenia accounted for 52.7%, while the R4 community complexity increased. Furthermore, the proportion of functional bacteria decreased, and the abundance of heterotrophic bacteria increased, which negatively affected the particle structure. In addition, the R3 microorganism has the best gene function expression level, which is significantly better than small particles in gene replication repair and energy conversion. Finally, the evolution of AnGS was analyzed through the OTU matrix between the samples. These results have some guiding significance for the optimization of the AnGS system and will be helpful for the application of the ANAMMOX process.
关键词 ：
anaerobic ammonium oxidation (ANAMMOX) anaerobic ammonium oxidation (ANAMMOX) gene function prediction gene function prediction microbial community structure microbial community structure particle size particle size specific activity specific activity
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| GB/T 7714 | Wang Xiao-Tong , Yang Hong . [Analysis of Performance and Microbial Diversity of ANAMMOX Sludge Based on Particle Size Differentiation]. [J]. | Huanjing kexue , 2021 , 42 (4) : 1930-1938 . |
| MLA | Wang Xiao-Tong et al. "[Analysis of Performance and Microbial Diversity of ANAMMOX Sludge Based on Particle Size Differentiation]." . | Huanjing kexue 42 . 4 (2021) : 1930-1938 . |
| APA | Wang Xiao-Tong , Yang Hong . [Analysis of Performance and Microbial Diversity of ANAMMOX Sludge Based on Particle Size Differentiation]. . | Huanjing kexue , 2021 , 42 (4) , 1930-1938 . |
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摘要 ：
The study aimed at evaluating the nitrogen removal performance of the immobilized denitrification filler, and the influence of shock loading on the high-rate denitrification process. A pilot scale reactor was operated for treatment of aniline production wastewater. The nitrogen removal activity significantly increased in the continuous feed experiments, reaching 5.23 kg N m-3 day-1 on day 31 (30 °C) at Hydraulic Retention Time (HRT) = 10 h. In the impact experiment, the denitrification filler was inhibited by Free Nitrite Acid (FNA) when the shock load flowed 1.5 times into the bioreactor and recovered after the load was restored for 20 h. The high-throughput results demonstrated that the dominant position of the denitrifying bacteria further enhanced in a micro toxic and high-salinity environment, providing a basis for the dominance of the composite denitrifying bacteria and the efficacy of the immobilization technology.
关键词 ：
High concentration nitrate nitrogen High concentration nitrate nitrogen High salinity aniline wastewater High salinity aniline wastewater Microbial community structure Microbial community structure Shock loading Shock loading
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| GB/T 7714 | He HaiChao , Yang Hong , Liu ZongYue et al. Denitrification performance and bacterial flora analysis of immobilized denitrification filler in industrial wastewater. [J]. | The Science of the total environment , 2021 , 764 : 142837 . |
| MLA | He HaiChao et al. "Denitrification performance and bacterial flora analysis of immobilized denitrification filler in industrial wastewater." . | The Science of the total environment 764 (2021) : 142837 . |
| APA | He HaiChao , Yang Hong , Liu ZongYue , Zhou YaKun , Li HongLi , Li ShiLei . Denitrification performance and bacterial flora analysis of immobilized denitrification filler in industrial wastewater. . | The Science of the total environment , 2021 , 764 , 142837 . |
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