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学者姓名：罗云敬
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摘要 ：
Fluorescence Resonance Energy Transfer (FRET) has emerged as a predominant, highly sensitive, and homogeneous optical analytical technique in the realm of analytical testing and bio-imaging. Gold nanoparticles (AuNPs) demonstrate a size-dependent, broader absorption range within visible wavelengths owing to the phenomenon of surface plasmon resonance. As a result, they can effectively act as light acceptors, enabling the creation of a donor-acceptor system crucial for achieving precise target analyte analysis. In this comprehensive review, we present an extensive survey of recent research advancements in the field of FRET techniques based on AuNPs for the analytical detection of a wide range of entities, including some biomolecules, pesticides, enzymes, microorganisms, food safety and environmental pollutants. Additionally, we elucidate the procedural strategies and underlying mechanisms involved. Finally, we provide perspectives on the current issues and future efforts surrounding the FRET applications of AuNPs in biological analysis. Overall, this review aims to provide a holistic comprehension of gold nanoparticle applications in life analysis using FRET, while also presenting a promising vision for future endeavors in this domain.
关键词 ：
FRET FRET Detection strategy Detection strategy Gold nanoparticles Gold nanoparticles Biosensors Biosensors
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| GB/T 7714 | Liu, Xuemei , Luo, Yunjing , Zhang, Yong et al. Gold nanoparticle-mediated fluorescence resonance energy transfer for analytical applications in the fields of life health and safety [J]. | TALANTA , 2024 , 282 . |
| MLA | Liu, Xuemei et al. "Gold nanoparticle-mediated fluorescence resonance energy transfer for analytical applications in the fields of life health and safety" . | TALANTA 282 (2024) . |
| APA | Liu, Xuemei , Luo, Yunjing , Zhang, Yong , Xie, Ziqi , Xu, Chao . Gold nanoparticle-mediated fluorescence resonance energy transfer for analytical applications in the fields of life health and safety . | TALANTA , 2024 , 282 . |
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摘要 ：
Lead pollution has remained a significant global concern for several decades due to its detrimental effects on the brain, heart, kidneys, lungs, and immune system across all age groups. Addressing the demand for detecting trace amounts of lead in food samples, we have developed a novel biosensor based on fluorescence resonance energy transfer (FRET) from fluorescein R6G to gold nanoclusters (AuNCs-CCY). By utilizing polypeptides as a template, we successfully synthesized AuNCs-CCY with an excitation spectrum that overlaps with the emission spectrum of R6G. Exploiting the fact that Pb 2 + induces the aggregation of gold nanoclusters, leading to the separation of R6G from AuNCs-CCY and subsequent fluorescence recovery, we achieved the quantitative detection of Pb 2 + . Within the concentration range of 0.002 -0.20 mu M, a linear relationship was observed between the fluorescence enhancement value (F -F 0 ) and Pb 2 + concentration, characterized by the linear equation y = 2398.69x + 87.87 (R 2 = 0.996). The limit of detection (LOD) for Pb 2 + was determined to be 0.00079 mu M (3 sigma/K). The recovery rate ranged from 96 % to 104 %, with a relative standard deviation (RSD) below 10 %. These findings demonstrate the potential application value of our biosensor, which offers a promising approach to address the urgent need for sensitive detection of heavy metal ions, specifically Pb 2 + , in food samples.
关键词 ：
Lead ion Lead ion Food sample Food sample Fluorescence resonance energy transfer Fluorescence resonance energy transfer Gold nanoclusters Gold nanoclusters
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| GB/T 7714 | Liu, Xuemei , Luo, Yunjing , Lin, Taifeng et al. Gold nanoclusters-based fluorescence resonance energy transfer for rapid and sensitive detection of Pb2+ [J]. | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2024 , 315 . |
| MLA | Liu, Xuemei et al. "Gold nanoclusters-based fluorescence resonance energy transfer for rapid and sensitive detection of Pb2+" . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 315 (2024) . |
| APA | Liu, Xuemei , Luo, Yunjing , Lin, Taifeng , Xie, Ziqi , Qi, Xiaohua . Gold nanoclusters-based fluorescence resonance energy transfer for rapid and sensitive detection of Pb2+ . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2024 , 315 . |
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摘要 ：
Glycoprotein 96 (gp96) is a highly conserved and ubiquitous glycoprotein that belongs to the heat shock protein 90 (HSP90) family. It comprises 4 domains: N-terminal domain (NTD), middle domain (MD), C-terminal domain (CTD) and charged linker region (CR). Each domain performs a specific function. NTD containing the nucleotide binding site interacts with and hydrolyzes ATP. MD is involved in client protein recognition, and is the site of constitutive dimerization. The ATP hydrolytic activity of NTD requires cooperative action of CR and MD. The schematic representation of gp96 topology is shown in the main text. An increased expression of gp96 has been reported in multiple cancers. Its upregulation in tumors is closely correlated with poor prognosis and decreased overall survival of patients, indicating that gp96 serves as a potential diagnostic and prognostic biomarker. As a chaperone protein gp96 directs the folding and/or assembly of secreted and membrane proteins. It has a limited client protein profile that is involved in key processes linked with the hallmarks of cancer. Previous studies have shown that cellular gp96 physically interacts with and directs the folding and assembly of several client proteins, including insulin-like growth factors (IGF), integrins, epidermal growth factor receptor-2 (HER2) and Wnt co-receptor LRP6, which are involved in the regulation of cell multiplication, normal tissue differentiation, cancer progression and metastasis. It has been found that gp96 was only expressed on cell surface of malignant tumor but not benign tissues. Cell membrane gp96 is closely associated with cancer cell proliferation, invasion, and metastasis. We further demonstrated that gp96 on cell membrane interacts with HER2, urokinase-type plasminogen activator-receptor (uPAR) or ER-alpha 36. Targeting gp96 by siRNA or a monoclonal antibody for gp96 led to decreased cell growth and invasion, increased apoptosis in vitro, and suppression of tumor growth in vivo, validating cell membrane gp96 as a therapeutic target. At present, the selective small-molecule inhibitors (NECA and PU-WS13), a gp96-specific monoclonal antibody (W9mAb), and an inhibitory gp96-targeted polypeptide (p37) are under development and their potential applications in the tumor targeting therapy are highlighted in this review.
关键词 ：
heat shock protein gp96 heat shock protein gp96 molecular chaperone molecular chaperone targeted therapy targeted therapy cancer cancer cell membrane gp96 cell membrane gp96
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| GB/T 7714 | Qian Li-Yuan , Li Chang-Fei , Luo Yun-Jing et al. Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression [J]. | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS , 2021 , 48 (9) : 993-1005 . |
| MLA | Qian Li-Yuan et al. "Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression" . | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 48 . 9 (2021) : 993-1005 . |
| APA | Qian Li-Yuan , Li Chang-Fei , Luo Yun-Jing , Meng Song-Dong . Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression . | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS , 2021 , 48 (9) , 993-1005 . |
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摘要 ：
目的 建立QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种常见农药残留的分析方法.方法 样品加入乙腈后涡旋混匀并超声提取,采用无水硫酸镁和N-丙基硅烷化硅胶(primary secondary amine,PSA)固相吸附剂净化,以乙腈-0.1％甲酸水溶液(V:V)作为流动相进行梯度洗脱,采用T3色谱柱(100 mm×2.1 mm,1.7μm)分离,在多反应监测(multiple reaction monitoring,MRM)模式下进行测定,外标法定量.结果 101种农药残留在3个添加水平(50、100和200μg/kg)下回收率在62.31％～119.74％之间,相对标准偏差小于15％,定量限为0.06～23.81μg/kg.结论 该方法操作简便、快速,有较高的灵敏度和准确度,可应用于主要原粮与油料中农药残留日常筛查工作.
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| GB/T 7714 | 王柄钧 , 史晓梅 , 张巍巍 et al. QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种农药残留 [J]. | 食品安全质量检测学报 , 2021 , 12 (18) : 7272-7280 . |
| MLA | 王柄钧 et al. "QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种农药残留" . | 食品安全质量检测学报 12 . 18 (2021) : 7272-7280 . |
| APA | 王柄钧 , 史晓梅 , 张巍巍 , 张江旭 , 赵文君 , 罗云敬 et al. QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种农药残留 . | 食品安全质量检测学报 , 2021 , 12 (18) , 7272-7280 . |
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摘要 ：
In this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed utilizing a surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs was characterized by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated that the diameter of the Gen-MMIPs was approximately 500 nm. Via analysis with a vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs was determined to be 24.79 emu g(-1). Fourier transform infrared (FT-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiments suggested that the genistein adsorption capability of Gen-MMIPs was 5.81 mg g(-1), and adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction (dSPE) adsorbents combined with HPLC were used to selectively separate genistein in soy sauce samples, and the recoveries ranged from 85.7 to 88.5% with relative standard deviations (RSDs) less than 5%, which proved that this method can be used for the detection of genistein residues in real samples.
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| GB/T 7714 | Xie, Ziqi , Luo, Yunjing , Na, Zhen et al. Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products [J]. | SCIENTIFIC REPORTS , 2021 , 11 (1) . |
| MLA | Xie, Ziqi et al. "Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products" . | SCIENTIFIC REPORTS 11 . 1 (2021) . |
| APA | Xie, Ziqi , Luo, Yunjing , Na, Zhen , Zhang, Wei , Zong, Yufei . Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products . | SCIENTIFIC REPORTS , 2021 , 11 (1) . |
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摘要 ：
This study aimed to assess the ameliorative effect and mechanisms of sulforaphane (SFN) on cadmium (Cd)induced hepatotoxicity. Four in vitro free radical scavenging tests, HepG2 cell viability analysis, and cadmium chloride (CdCl2) indeced liver injured mice mode were employed. SFN scavenged the free radicals in vitro, and mitigated the oxidative damages in HepG2 cells incubated with SFN + CdCl2. In mice models, SFN pretreatment dose-dependently remarkably improved redox homeostasis, and attenuated the expressions of the key liver inflammatory factors (TNF-alpha, IL-6, IL-1 beta) by CdCl2. The pathological examinations were consistent with the above results. Moreover, both mRNA and protein expression of hepatic nuclear factor-erythroid 2-related factor 2 and hemeoxygenase-1 increased, while nuclear factor-kappa B reduced in Cd + SFN co-treated groups. Taken together, this study firstly demonstrated the anti-oxidant and anti-inflammatory effects of SFN against Cd induced liver damages, which associated with the modulation of intrinsic Nrf2/ARE and NF-kappa B pathway.
关键词 ：
Inflammation Inflammation Hepatotoxicity Hepatotoxicity Sulforaphen Sulforaphen Cadmium Cadmium Oxidative stress Oxidative stress
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| GB/T 7714 | He, Qingfeng , Luo, Yunjing , Xie, Ziqi . Sulforaphane ameliorates cadmium induced hepatotoxicity through the up-regulation of /Nrf2/ARE pathway and the inactivation of NF-kappa B [J]. | JOURNAL OF FUNCTIONAL FOODS , 2021 , 77 . |
| MLA | He, Qingfeng et al. "Sulforaphane ameliorates cadmium induced hepatotoxicity through the up-regulation of /Nrf2/ARE pathway and the inactivation of NF-kappa B" . | JOURNAL OF FUNCTIONAL FOODS 77 (2021) . |
| APA | He, Qingfeng , Luo, Yunjing , Xie, Ziqi . Sulforaphane ameliorates cadmium induced hepatotoxicity through the up-regulation of /Nrf2/ARE pathway and the inactivation of NF-kappa B . | JOURNAL OF FUNCTIONAL FOODS , 2021 , 77 . |
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摘要 ：
Peroxynitrite is known as a strong deleterious species that may readily trigger several geriatric diseases via injuring cellular constituents. Proanthocyanidins, a biological flavonoids constituent of Pinus sylvestris L. bark, has been attributed a large variety of pharmacological functions to its antioxidant potential. The results revealed that peroxynitrite could cause the generation of hydroxyl radical, the breakage of φX-174 plasmid DNA brand as well as the nitration of L-tyrosine. However, pine (Pinus sylvestris L.) bark proanthocyanidins extracts at low concentration range markedly inhibited the peroxynitrite -induced the formation of open circular DNA form (IC50 = 5.03±0.39 mg/mL). The 3-Nitro-L-tyrosine generated by the reaction of peroxynitrite with L-tyrosine was reduced by PBP (IC50 = 1.01±0.01 mg/mL). Besides, electron spin resonance spectroscopy data indicates that the intensive signal of dimethyl pyridine N-oxide hydroxyl radical adduct from peroxynitrite was reversed by pine bark proanthocyanidins extracts (IC50 =1.02±0.04 mg/mL). Moreover, the obtained data shows that PBP provides more efficient protection against peroxynitrite than that of ascorbic acid. Together, the present study suggests that pine bark proanthocyanidins could exert potent preventive activity against peroxynitrite -elicited cytotoxicity on the biomacromolecules, a study-worthy finding with pharmacological importance.
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| GB/T 7714 | He Qingfeng , Luo Yunjing , Shi Jianlong et al. Pine (Pinus sylvestris L.) bark proanthocyanidins affords prevention of peroxynitrite-induced l-tyrosine nitration, DNA damage and hydroxyl radical formation. [J]. | Pakistan journal of pharmaceutical sciences , 2020 , 33 (1) : 141-148 . |
| MLA | He Qingfeng et al. "Pine (Pinus sylvestris L.) bark proanthocyanidins affords prevention of peroxynitrite-induced l-tyrosine nitration, DNA damage and hydroxyl radical formation." . | Pakistan journal of pharmaceutical sciences 33 . 1 (2020) : 141-148 . |
| APA | He Qingfeng , Luo Yunjing , Shi Jianlong , Tang Xiaoyu , Wei Anqi . Pine (Pinus sylvestris L.) bark proanthocyanidins affords prevention of peroxynitrite-induced l-tyrosine nitration, DNA damage and hydroxyl radical formation. . | Pakistan journal of pharmaceutical sciences , 2020 , 33 (1) , 141-148 . |
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| GB/T 7714 | Zhang, Congxiao , Luo, Yunjing . Kinetics of peroxynitrite-induced nitrification of tyrosine catalyzed by iron porphyrin [J]. | BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY , 2020 , 127 : 240-240 . |
| MLA | Zhang, Congxiao et al. "Kinetics of peroxynitrite-induced nitrification of tyrosine catalyzed by iron porphyrin" . | BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY 127 (2020) : 240-240 . |
| APA | Zhang, Congxiao , Luo, Yunjing . Kinetics of peroxynitrite-induced nitrification of tyrosine catalyzed by iron porphyrin . | BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY , 2020 , 127 , 240-240 . |
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摘要 ：
Peroxynitrite is known as a strong deleterious species that may readily trigger several geriatric diseases via injuring cellular constituents. Proanthocyanidins, a biological flavonoids constituent of Pinus sylvestris L. bark, has been attributed a large variety of phaimacological functions to its antioxidant potential. The results revealed that peroxynitrite could cause the generation of hydroxyl radical, the breakage of phi X-174 plasmid DNA brand as well as the nitration of L-tyrosine. However, pine (Pinus sylvestris L.) bark proanthocyanidins extracts at low concentration range markedly inhibited the peroxynitrite -induced the formation of open circular DNA form (IC50 = 5.03 +/- 0.39 mg/mL). The 3-Nitro-L-tyrosine generated by the reaction of peroxynitrite with L -tyrosine was reduced by PBP (IC50 = 1.01 +/- 0.01 mg/mL). Besides, electron spin resonance spectroscopy data indicates that the intensive signal of dimethyl pyridine N-oxide hydroxyl radical adduct from peroxynitrite was reversed by pine bark proanthocyanidins extracts (IC50 =1.02 +/- 0.04 mg/mL). Moreover, the obtained data shows that PBP provides more efficient protection against peroxynitrite than that of ascorbic acid. Together, the present study suggests that pine bark proanthocyanidins could exert potent preventive activity against peroxynitrite -elicited cytotoxicity on the biomacromolecules, a study-worthy finding with pharmacological importance.
关键词 ：
antioxidant antioxidant peroxynitrite peroxynitrite Pine (Pinus sylvestris L.) Pine (Pinus sylvestris L.) proanthocyanidins proanthocyanidins
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| GB/T 7714 | He, Qingfeng , Luo, Yunjing , Shi, Jianlong et al. Pine (Pinus sylvestris L.) bark proanthocyanidins affords prevention of peroxynitrite-induced 1-tyrosine nitration, DNA damage and hydroxyl radical formation [J]. | PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES , 2020 , 33 (1) : 141-148 . |
| MLA | He, Qingfeng et al. "Pine (Pinus sylvestris L.) bark proanthocyanidins affords prevention of peroxynitrite-induced 1-tyrosine nitration, DNA damage and hydroxyl radical formation" . | PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES 33 . 1 (2020) : 141-148 . |
| APA | He, Qingfeng , Luo, Yunjing , Shi, Jianlong , Tang, Xiaoyu , Wei, Anqi . Pine (Pinus sylvestris L.) bark proanthocyanidins affords prevention of peroxynitrite-induced 1-tyrosine nitration, DNA damage and hydroxyl radical formation . | PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES , 2020 , 33 (1) , 141-148 . |
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摘要 ：
Both clenbuterol (CLB) and ractopamine (RAC) are beta-adrenergic agonists. After long-term excessive intake, there will be adverse reactions such as headache, chest tightness, limb numbness, and serious lifethreatening. Simultaneous detection of CLB and RAC in related samples is of great importance for human health. In this work, we outline a microfluidics-based indirect competitive immunoassay (MICI) system that can sensitively detect residual CLB and RAC in pork, swine blood and swine urine. The rapid detection of multiple samples can be achieved in one chip, which greatly improves the detection efficiency. This method has good stability and reproducibility and the microfluidic chips are easy to manufacture. The linear ranges for CLB and RAC detection by MICI are 0.1-2.5 ng/mL and 0.1-5 ng/mL, and the limits of detection (LODs) are 0.094 ng/mL and 0.091 ng/mL, respectively. This straightforward and portable immunoassay system provides a good platform for rapid detection of harmful substances in food samples. (C) 2020 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
关键词 ：
Microfluidic chips Microfluidic chips Indirect competitive immunoassay Indirect competitive immunoassay Clenbuterol Clenbuterol Ractopamine Ractopamine Simultaneous detection Simultaneous detection
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| GB/T 7714 | Wang, Qi , Deng, Jinqi , Chen, Yiping et al. An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine [J]. | CHINESE CHEMICAL LETTERS , 2020 , 31 (7) : 1835-1838 . |
| MLA | Wang, Qi et al. "An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine" . | CHINESE CHEMICAL LETTERS 31 . 7 (2020) : 1835-1838 . |
| APA | Wang, Qi , Deng, Jinqi , Chen, Yiping , Luo, Yunjing , Jiang, Xingyu . An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine . | CHINESE CHEMICAL LETTERS , 2020 , 31 (7) , 1835-1838 . |
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