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学者姓名：罗云敬
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摘要 ：
Glycoprotein 96 (gp96) is a highly conserved and ubiquitous glycoprotein that belongs to the heat shock protein 90 (HSP90) family. It comprises 4 domains: N-terminal domain (NTD), middle domain (MD), C-terminal domain (CTD) and charged linker region (CR). Each domain performs a specific function. NTD containing the nucleotide binding site interacts with and hydrolyzes ATP. MD is involved in client protein recognition, and is the site of constitutive dimerization. The ATP hydrolytic activity of NTD requires cooperative action of CR and MD. The schematic representation of gp96 topology is shown in the main text. An increased expression of gp96 has been reported in multiple cancers. Its upregulation in tumors is closely correlated with poor prognosis and decreased overall survival of patients, indicating that gp96 serves as a potential diagnostic and prognostic biomarker. As a chaperone protein gp96 directs the folding and/or assembly of secreted and membrane proteins. It has a limited client protein profile that is involved in key processes linked with the hallmarks of cancer. Previous studies have shown that cellular gp96 physically interacts with and directs the folding and assembly of several client proteins, including insulin-like growth factors (IGF), integrins, epidermal growth factor receptor-2 (HER2) and Wnt co-receptor LRP6, which are involved in the regulation of cell multiplication, normal tissue differentiation, cancer progression and metastasis. It has been found that gp96 was only expressed on cell surface of malignant tumor but not benign tissues. Cell membrane gp96 is closely associated with cancer cell proliferation, invasion, and metastasis. We further demonstrated that gp96 on cell membrane interacts with HER2, urokinase-type plasminogen activator-receptor (uPAR) or ER-alpha 36. Targeting gp96 by siRNA or a monoclonal antibody for gp96 led to decreased cell growth and invasion, increased apoptosis in vitro, and suppression of tumor growth in vivo, validating cell membrane gp96 as a therapeutic target. At present, the selective small-molecule inhibitors (NECA and PU-WS13), a gp96-specific monoclonal antibody (W9mAb), and an inhibitory gp96-targeted polypeptide (p37) are under development and their potential applications in the tumor targeting therapy are highlighted in this review.
关键词 ：
heat shock protein gp96 heat shock protein gp96 molecular chaperone molecular chaperone targeted therapy targeted therapy cancer cancer cell membrane gp96 cell membrane gp96
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| GB/T 7714 | Qian Li-Yuan , Li Chang-Fei , Luo Yun-Jing et al. Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression [J]. | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS , 2021 , 48 (9) : 993-1005 . |
| MLA | Qian Li-Yuan et al. "Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression" . | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 48 . 9 (2021) : 993-1005 . |
| APA | Qian Li-Yuan , Li Chang-Fei , Luo Yun-Jing , Meng Song-Dong . Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression . | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS , 2021 , 48 (9) , 993-1005 . |
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摘要 ：
肝细胞癌(Hepatocellular carcinoma,HCC)是致死率第3的恶性肿瘤,也是全球第5大常见癌症。肝癌在临床上的治疗手段非常有限,患者的总生存率也很低。因此,肝癌的早期诊断和治疗对于患者总生存率有着重要的影响。甲胎蛋白(Alpha-fetoprotein,AFP)是最早发现也是目前应用最广泛的肝癌标志物之一。目前,多项研究表明,作为一个特异性的癌基因,AFP在肝癌的发生、发展、诊断和治疗中有巨大的研究价值。文中简述了AFP在肝癌发生发展中的分子调控机制以及在肝癌细胞逃避免疫监视中的作用,着重阐述AFP作为重要的肝癌靶标分子在肝癌的临床诊断和治疗研究中的应用。
关键词 ：
甲胎蛋白 甲胎蛋白 肝癌治疗 肝癌治疗 肝癌 肝癌 临床诊断 临床诊断 血清学标志物 血清学标志物
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| GB/T 7714 | 钱丽媛 , 李长菲 , 罗云敬 et al. 甲胎蛋白在肝癌的诊断和治疗中的研究进展 [J]. | 生物工程学报 , 2021 , 37 (09) : 3042-3060 . |
| MLA | 钱丽媛 et al. "甲胎蛋白在肝癌的诊断和治疗中的研究进展" . | 生物工程学报 37 . 09 (2021) : 3042-3060 . |
| APA | 钱丽媛 , 李长菲 , 罗云敬 , 孟颂东 . 甲胎蛋白在肝癌的诊断和治疗中的研究进展 . | 生物工程学报 , 2021 , 37 (09) , 3042-3060 . |
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摘要 ：
基于分步液液提取以及脂质净化柱净化的样品前处理方式,建立同时测定食用植物油中28种真菌毒素的高效液相色谱-串联质谱的测定方法。28种真菌毒素的定量限范围0.07～1.25μg/kg,三水平加标回收率范围60%～120%,峰面积相对标准偏差小于12.5%,均满足国家标准GB/T 27404-2008《实验室质量控制规范食品理化检测》中实验室质量控制规范以及真菌毒素限量标准的要求。应用本方法对市售食用植物油样品进行真菌毒素的风险监测,结果显示:黄曲霉毒素、玉米赤霉烯酮及其衍生物、杂色曲霉素、T-2毒素有不同程度的检出。其中,花生油中主要的真菌毒素种类为黄曲霉毒素、杂色曲霉素以及玉米赤霉烯酮;菜籽油...
关键词 ：
真菌毒素 真菌毒素 脂质净化 脂质净化 风险监测 风险监测 食用油 食用油
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| GB/T 7714 | 杨帅 , 杨永坛 , 穆蕾 et al. 液相色谱-串联质谱同时测定食用植物油中的多种真菌毒素 [J]. | 中国食品学报 , 2021 , 21 (05) : 309-316 . |
| MLA | 杨帅 et al. "液相色谱-串联质谱同时测定食用植物油中的多种真菌毒素" . | 中国食品学报 21 . 05 (2021) : 309-316 . |
| APA | 杨帅 , 杨永坛 , 穆蕾 , 杨霞 , 杨悠悠 , 罗云敬 . 液相色谱-串联质谱同时测定食用植物油中的多种真菌毒素 . | 中国食品学报 , 2021 , 21 (05) , 309-316 . |
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摘要 ：
目的:建立QuEChERS-气相色谱-串联质谱(GC-MS/MS)快速测定谷类中179种农药残留的方法,并应用于191份谷类样品。方法:样品经乙腈提取,N-丙基乙二胺(PSA)和十八烷基硅烷键合硅胶(C_(18))净化,气相色谱-串联质谱多反应离子监测(MRM)模式检测。结果:179种农药在质量浓度为0.02～0.5 mg/L范围内线性关系良好,相关系数均大于0.996,检出限和定量限分别为0.001～0.016 mg/kg和0.003～0.050 mg/kg,在0.025 mg/L、0.050 mg/L和0.100 mg/L添加水平下,农药的回收率为63.7%～117.2%,相对标准偏差(R...
关键词 ：
气相色谱-串联质谱法 气相色谱-串联质谱法 谷类 谷类 测定 测定 农药残留 农药残留 QuEChERS QuEChERS
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| GB/T 7714 | 席甲甲 , 张巍巍 , 王柄钧 et al. 气相色谱-串联质谱法快速测定谷类中179种农药残留 [J]. | 食品安全导刊 , 2021 , (18) : 137-139 . |
| MLA | 席甲甲 et al. "气相色谱-串联质谱法快速测定谷类中179种农药残留" . | 食品安全导刊 18 (2021) : 137-139 . |
| APA | 席甲甲 , 张巍巍 , 王柄钧 , 史晓梅 , 罗云敬 , 钱承敬 . 气相色谱-串联质谱法快速测定谷类中179种农药残留 . | 食品安全导刊 , 2021 , (18) , 137-139 . |
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摘要 ：
热休克蛋白90B1又称为糖蛋白96 (gp96). gp96属于热休克蛋白90家族,是一种高度保守且普遍存在的糖蛋白.作为一种内质网蛋白,gp96在维持内质网稳态、内质网应激、钙稳态等方面起着重要的调控作用,这些调控网络在肿瘤的发生发展过程中起着重要的作用. gp96作为分子伴侣在稳定和激活客户蛋白等方面有众多报道,其中包括HER2、整合素和Toll样受体等多个客户蛋白.大量研究表明,gp96在肝癌、乳腺癌、胃癌等不同类型的肿瘤中高表达,并在肿瘤的生长、侵袭和转移等方面起着重要的作用.本文从gp96的基本结构和功能及其在肿瘤发生发展中的作用等方面进行综述,并着重阐述细胞膜gp96相关的研究进展...
关键词 ：
分子伴侣 分子伴侣 热休克蛋白gp96 热休克蛋白gp96 肿瘤 肿瘤 胞膜gp96 胞膜gp96 靶向治疗 靶向治疗
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| GB/T 7714 | 钱丽媛 , 李长菲 , 罗云敬 et al. 热休克蛋白gp96在恶性肿瘤中的研究进展 [J]. | 生物化学与生物物理进展 , 2021 , 48 (09) : 993-1005 . |
| MLA | 钱丽媛 et al. "热休克蛋白gp96在恶性肿瘤中的研究进展" . | 生物化学与生物物理进展 48 . 09 (2021) : 993-1005 . |
| APA | 钱丽媛 , 李长菲 , 罗云敬 , 孟颂东 . 热休克蛋白gp96在恶性肿瘤中的研究进展 . | 生物化学与生物物理进展 , 2021 , 48 (09) , 993-1005 . |
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摘要 ：
目的 建立QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种常见农药残留的分析方法.方法 样品加入乙腈后涡旋混匀并超声提取,采用无水硫酸镁和N-丙基硅烷化硅胶(primary secondary amine,PSA)固相吸附剂净化,以乙腈-0.1％甲酸水溶液(V:V)作为流动相进行梯度洗脱,采用T3色谱柱(100 mm×2.1 mm,1.7μm)分离,在多反应监测(multiple reaction monitoring,MRM)模式下进行测定,外标法定量.结果 101种农药残留在3个添加水平(50、100和200μg/kg)下回收率在62.31％～119.74％之间,相对标准偏差小于15％,定量限为0.06～23.81μg/kg.结论 该方法操作简便、快速,有较高的灵敏度和准确度,可应用于主要原粮与油料中农药残留日常筛查工作.
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| GB/T 7714 | 王柄钧 , 史晓梅 , 张巍巍 et al. QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种农药残留 [J]. | 食品安全质量检测学报 , 2021 , 12 (18) : 7272-7280 . |
| MLA | 王柄钧 et al. "QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种农药残留" . | 食品安全质量检测学报 12 . 18 (2021) : 7272-7280 . |
| APA | 王柄钧 , 史晓梅 , 张巍巍 , 张江旭 , 赵文君 , 罗云敬 et al. QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种农药残留 . | 食品安全质量检测学报 , 2021 , 12 (18) , 7272-7280 . |
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摘要 ：
This study aimed to assess the ameliorative effect and mechanisms of sulforaphane (SFN) on cadmium (Cd)induced hepatotoxicity. Four in vitro free radical scavenging tests, HepG2 cell viability analysis, and cadmium chloride (CdCl2) indeced liver injured mice mode were employed. SFN scavenged the free radicals in vitro, and mitigated the oxidative damages in HepG2 cells incubated with SFN + CdCl2. In mice models, SFN pretreatment dose-dependently remarkably improved redox homeostasis, and attenuated the expressions of the key liver inflammatory factors (TNF-alpha, IL-6, IL-1 beta) by CdCl2. The pathological examinations were consistent with the above results. Moreover, both mRNA and protein expression of hepatic nuclear factor-erythroid 2-related factor 2 and hemeoxygenase-1 increased, while nuclear factor-kappa B reduced in Cd + SFN co-treated groups. Taken together, this study firstly demonstrated the anti-oxidant and anti-inflammatory effects of SFN against Cd induced liver damages, which associated with the modulation of intrinsic Nrf2/ARE and NF-kappa B pathway.
关键词 ：
Cadmium Cadmium Hepatotoxicity Hepatotoxicity Inflammation Inflammation Oxidative stress Oxidative stress Sulforaphen Sulforaphen
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| GB/T 7714 | He, Qingfeng , Luo, Yunjing , Xie, Ziqi . Sulforaphane ameliorates cadmium induced hepatotoxicity through the up-regulation of /Nrf2/ARE pathway and the inactivation of NF-kappa B [J]. | JOURNAL OF FUNCTIONAL FOODS , 2021 , 77 . |
| MLA | He, Qingfeng et al. "Sulforaphane ameliorates cadmium induced hepatotoxicity through the up-regulation of /Nrf2/ARE pathway and the inactivation of NF-kappa B" . | JOURNAL OF FUNCTIONAL FOODS 77 (2021) . |
| APA | He, Qingfeng , Luo, Yunjing , Xie, Ziqi . Sulforaphane ameliorates cadmium induced hepatotoxicity through the up-regulation of /Nrf2/ARE pathway and the inactivation of NF-kappa B . | JOURNAL OF FUNCTIONAL FOODS , 2021 , 77 . |
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摘要 ：
An liquid chromatography-tandem mass spectrometry method coupled with a stable isotope dilution assay was established for the simultaneous detection of 17 mycotoxins and their derivatives (aflatoxins B1 , B2 , G1 , G2 , M1 , and M2 ; fumonisins B1 and B2 ; ochratoxin A; zearalenone; zearalanone; α-zearalanol; α-zearalenol; T-2 toxin; deoxynivalenol; deepoxy-deoxynivalenol; and sterigmatocystin) in milk and dairy products. The mycotoxins were extracted with acidified acetonitrile and the lipids were removed using a Captiva EMR-lipid column. The average recoveries of the target compounds from samples spiked at three different concentrations were 67-102%, and the relative standard deviations of the peak areas were less than 10%. Limits of quantification (S/N = 10) of 0.004-1.25 μg/kg were achieved, which are significantly lower than the maximum levels allowed in various countries and regions for each regulated mycotoxin. Milk and yogurt products from local markets and e-commercial platforms were analyzed using the optimized method. The screening showed that aflatoxin M1 , deoxynivalenol, fumonisins B1 and B2 , and zearalenone could be found in milk and yogurt products, especially those products also containing grains or jujube ingredients, indicating that there is a risk of mycotoxins in dairy products.
关键词 ：
dairy dairy isotope dilution isotope dilution multimycotoxins multimycotoxins risk assessment risk assessment
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| GB/T 7714 | Yang Shuai , Luo Yunjing , Mu Lei et al. Risk screening of mycotoxins and their derivatives in dairy products using a stable isotope dilution assay and LC-MS/MS. [J]. | Journal of separation science , 2021 , 44 (4) : 782-792 . |
| MLA | Yang Shuai et al. "Risk screening of mycotoxins and their derivatives in dairy products using a stable isotope dilution assay and LC-MS/MS." . | Journal of separation science 44 . 4 (2021) : 782-792 . |
| APA | Yang Shuai , Luo Yunjing , Mu Lei , Yang Youyou , Yang Yongtan . Risk screening of mycotoxins and their derivatives in dairy products using a stable isotope dilution assay and LC-MS/MS. . | Journal of separation science , 2021 , 44 (4) , 782-792 . |
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摘要 ：
Peroxynitrite is known as a strong deleterious species that may readily trigger several geriatric diseases via injuring cellular constituents. Proanthocyanidins, a biological flavonoids constituent of Pinus sylvestris L. bark, has been attributed a large variety of pharmacological functions to its antioxidant potential. The results revealed that peroxynitrite could cause the generation of hydroxyl radical, the breakage of φX-174 plasmid DNA brand as well as the nitration of L-tyrosine. However, pine (Pinus sylvestris L.) bark proanthocyanidins extracts at low concentration range markedly inhibited the peroxynitrite -induced the formation of open circular DNA form (IC50 = 5.03±0.39 mg/mL). The 3-Nitro-L-tyrosine generated by the reaction of peroxynitrite with L-tyrosine was reduced by PBP (IC50 = 1.01±0.01 mg/mL). Besides, electron spin resonance spectroscopy data indicates that the intensive signal of dimethyl pyridine N-oxide hydroxyl radical adduct from peroxynitrite was reversed by pine bark proanthocyanidins extracts (IC50 =1.02±0.04 mg/mL). Moreover, the obtained data shows that PBP provides more efficient protection against peroxynitrite than that of ascorbic acid. Together, the present study suggests that pine bark proanthocyanidins could exert potent preventive activity against peroxynitrite -elicited cytotoxicity on the biomacromolecules, a study-worthy finding with pharmacological importance.
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| GB/T 7714 | He Qingfeng , Luo Yunjing , Shi Jianlong et al. Pine (Pinus sylvestris L.) bark proanthocyanidins affords prevention of peroxynitrite-induced l-tyrosine nitration, DNA damage and hydroxyl radical formation. [J]. | Pakistan journal of pharmaceutical sciences , 2020 , 33 (1) : 141-148 . |
| MLA | He Qingfeng et al. "Pine (Pinus sylvestris L.) bark proanthocyanidins affords prevention of peroxynitrite-induced l-tyrosine nitration, DNA damage and hydroxyl radical formation." . | Pakistan journal of pharmaceutical sciences 33 . 1 (2020) : 141-148 . |
| APA | He Qingfeng , Luo Yunjing , Shi Jianlong , Tang Xiaoyu , Wei Anqi . Pine (Pinus sylvestris L.) bark proanthocyanidins affords prevention of peroxynitrite-induced l-tyrosine nitration, DNA damage and hydroxyl radical formation. . | Pakistan journal of pharmaceutical sciences , 2020 , 33 (1) , 141-148 . |
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| GB/T 7714 | Zhang, Congxiao , Luo, Yunjing . Kinetics of peroxynitrite-induced nitrification of tyrosine catalyzed by iron porphyrin [J]. | BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY , 2020 , 127 : 240-240 . |
| MLA | Zhang, Congxiao et al. "Kinetics of peroxynitrite-induced nitrification of tyrosine catalyzed by iron porphyrin" . | BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY 127 (2020) : 240-240 . |
| APA | Zhang, Congxiao , Luo, Yunjing . Kinetics of peroxynitrite-induced nitrification of tyrosine catalyzed by iron porphyrin . | BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY , 2020 , 127 , 240-240 . |
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