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学者姓名:江竹青
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摘要 :
A single-shot dual-wavelength digital holographic microscopy with an adjustable off-axis configuration is presented, which helps realize real-time quantitative phase imaging for living cells. With this configuration, two sets of interference fringes corresponding to their wavelengths can be flexibly recorded onto one hologram in one shot. The universal expression on the dual-wavelength hologram recorded under any wave vector orientation angles of reference beams is given. To avoid as much as possible the effect of zero-order spectrum, we can flexibly select their carry frequencies for the two wavelengths using this adjustable off-axis configuration, according to the distribution feature of object's spatial-frequency spectrum. This merit is verified by a quantitative phase imaging experiment for the microchannel of a microfluidic chip. The reconstructed phase maps of living onion epidermal cells exhibit cellular internal life activities, for the first time to the best of our knowledge, vividly displaying the progress of the nucleus, cell wall, cytoskeleton, and the substance transport in microtubules inside living cells. These imaging results demonstrate the availability and reliability of the presented method for real-time quantitative phase imaging. (C) 2021 Optical Society of America
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GB/T 7714 | Huang, Meng , Qin, Hongpeng , Jiang, Zhuqing . Real-time quantitative phase imaging by single-shot dual-wavelength off-axis digital holographic microscopy [J]. | APPLIED OPTICS , 2021 , 60 (15) : 4418-4425 . |
MLA | Huang, Meng 等. "Real-time quantitative phase imaging by single-shot dual-wavelength off-axis digital holographic microscopy" . | APPLIED OPTICS 60 . 15 (2021) : 4418-4425 . |
APA | Huang, Meng , Qin, Hongpeng , Jiang, Zhuqing . Real-time quantitative phase imaging by single-shot dual-wavelength off-axis digital holographic microscopy . | APPLIED OPTICS , 2021 , 60 (15) , 4418-4425 . |
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摘要 :
An optimization algorithm is developed to extend the unambiguous range of DWDH, which is suitable for imaging living onion epidermal cells, and meanwhile to reduce the noise. In the experiments, the holograms with two wavelengths can be acquired in a single shot recording by using off-axis dual-wavelength digital holography, resulting to obtain their two sets of spatial spectrum via Fourier transform for these two holograms. The denoised unwrapping phase image of sample is straightforward reconstructed with the optimization algorithm, instead of increasing the noise due to longer synthetic wavelength. As a result, the unwrapped phase images of onion epidermal cells are achieved.
关键词 :
phase unwrapping phase unwrapping dual-wavelength digital holography dual-wavelength digital holography phase contrast phase contrast onion epidermal cells onion epidermal cells Digital holographic microscopy Digital holographic microscopy
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GB/T 7714 | Qin, Hongpeng , Qin, Rikang , Jiang, Zhuqing . Improvement and denoising for Dual-Wavelength Digital Holographic Microscopy [C] . 2020 . |
MLA | Qin, Hongpeng 等. "Improvement and denoising for Dual-Wavelength Digital Holographic Microscopy" . (2020) . |
APA | Qin, Hongpeng , Qin, Rikang , Jiang, Zhuqing . Improvement and denoising for Dual-Wavelength Digital Holographic Microscopy . (2020) . |
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摘要 :
一种基于克罗内克积插值的离轴数字全息重建方法,涉及数字全息成像技术领域。本发明记录样品的离轴数字全息图,采用一个常数矩阵对离轴数字全息图作克罗内克积插值处理,对插值后的全息图作傅里叶变换得到包括新增混叠频谱区域的插值频谱图,将混叠频谱区域相加并用其替换原有区域分布,截取混叠频谱区域中的正一级或负一级频谱项移动到频谱域中心,作逆傅里叶变换后进行角谱传播重建复振幅分布,得到成像重建的振幅图和相位图。本方法有利于由样品的离轴数字全息图重建其高分辨的振幅分布和相位分布。
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GB/T 7714 | 刘毅伟 , 江竹青 , 王医博 et al. 基于克罗内克积插值的离轴数字全息成像重建方法 : CN202011365760.4[P]. | 2020-11-29 . |
MLA | 刘毅伟 et al. "基于克罗内克积插值的离轴数字全息成像重建方法" : CN202011365760.4. | 2020-11-29 . |
APA | 刘毅伟 , 江竹青 , 王医博 , 孙秋雅 , 陈浩 , 秦宏鹏 . 基于克罗内克积插值的离轴数字全息成像重建方法 : CN202011365760.4. | 2020-11-29 . |
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摘要 :
In this paper, an off-axis digital holography multi-frame image super-resolution reconstruction method is presented. Each low-resolution hologram will have small displacement in different directions. The obtained low-resolution holograms are processed iteratively with the super-resolution algorithm to obtain the super-resolution hologram, and then the amplitude image is reconstructed from the super-resolution hologram. The imaging results show that the resolution of the reconstructed image after super-resolution processing is obviously improved, because of increase of high-frequency information in the high-resolution hologram.
关键词 :
Digital holography Digital holography imaging reconstruction algorithm imaging reconstruction algorithm imaging resolution imaging resolution phase reconstruction phase reconstruction super-resolution imaging super-resolution imaging
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GB/T 7714 | Wang, Yibo , Liu, Yiwei , Chen, Hao et al. Optimization of High-Resolution Reconstruction Algorithm for Off-axis Digital Holographic Imaging [C] . 2020 . |
MLA | Wang, Yibo et al. "Optimization of High-Resolution Reconstruction Algorithm for Off-axis Digital Holographic Imaging" . (2020) . |
APA | Wang, Yibo , Liu, Yiwei , Chen, Hao , Sun, Qiuya , Jiang, Zhuqing . Optimization of High-Resolution Reconstruction Algorithm for Off-axis Digital Holographic Imaging . (2020) . |
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摘要 :
We present a method of single-frame reconstruction in off-axis digital holography with Kronecker-product interpolation to maximize spatial-frequency extraction. The Kronecker-product interpolation operated on a one-frame off-axis digital hologram can generate several aliasing spectra in its Fourier domain, where the zero-order aliasing spectra in the extrapolation region can be suppressed effectively. After adding all aliasing spectrum regions and substituting each aliasing spectrum in place with their sum, spectrum interception with a larger scope for filtering can be made in one of the aliasing spectrum regions. The experimental results demonstrate that the method is valid to improve the resolution in off-axis digital holography. (C) 2020 Optical Society of America
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GB/T 7714 | Liu, Yiwei , Jiang, Zhuqing , Wang, Yibo et al. Single-frame reconstruction for improvement of off-axis digital holographic imaging based on image interpolation [J]. | OPTICS LETTERS , 2020 , 45 (24) : 6623-6626 . |
MLA | Liu, Yiwei et al. "Single-frame reconstruction for improvement of off-axis digital holographic imaging based on image interpolation" . | OPTICS LETTERS 45 . 24 (2020) : 6623-6626 . |
APA | Liu, Yiwei , Jiang, Zhuqing , Wang, Yibo , Sun, Qiuya , Chen, Hao . Single-frame reconstruction for improvement of off-axis digital holographic imaging based on image interpolation . | OPTICS LETTERS , 2020 , 45 (24) , 6623-6626 . |
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摘要 :
We introduce a new STDH modality to record and process hybrid space-time representations. This allows improving resolution with one single object scan. Onion epidermal cells has been used as samples to verify this approach. © OSA 2019 © 2019 The Author(s)
关键词 :
Holography Holography Imaging systems Imaging systems
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GB/T 7714 | Wang, Zhe , Bianco, Vittorio , Cui, Yutong et al. 2D resolution enhancement from 1D scanning Space-Time Digital Holography (STDH) [C] . 2019 . |
MLA | Wang, Zhe et al. "2D resolution enhancement from 1D scanning Space-Time Digital Holography (STDH)" . (2019) . |
APA | Wang, Zhe , Bianco, Vittorio , Cui, Yutong , Paturzo, Melania , Jiang, Zhuqing , Ferraro, Pietro . 2D resolution enhancement from 1D scanning Space-Time Digital Holography (STDH) . (2019) . |
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摘要 :
The optimization of image resolution for digital holographic scanning imaging of biological cells is investigated. Digital holographic scanning imaging experiments on the upper epidermal cells of onions are performed to demonstrate the validity of resolution optimization algorithm. In the experiments, the holograms of the upper epidermal cells of onion are recorded at a certain scanning rate, and then are processed by using the resolution optimization algorithm. As a result, the phase images of the onion epidermal cells with higher contrast and resolution are obtained. According to the synthetic holograms, the changes of cell nucleus and actin microfilament inside onion's epidermal cells are displayed. In addition, the dehydration process and plasmolysis phenomenon inside onion epidermal cells are also exhibited by recording long-term scanning holograms of living epidermal cells. The experimental results demonstrate that image quality of living onion epidermal cells can be improved by optimizing the algorithms.
关键词 :
digital holographic microscopy digital holographic microscopy image resolution image resolution onion epidermal cells onion epidermal cells phase contrast phase contrast
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GB/T 7714 | Qin, Rikang , Jia, Yanyan , Liu, Yiwei et al. Imaging Characteristic Optimization of Digital Holographic microscopy for Onion Epidermal Cells [C] . 2019 . |
MLA | Qin, Rikang et al. "Imaging Characteristic Optimization of Digital Holographic microscopy for Onion Epidermal Cells" . (2019) . |
APA | Qin, Rikang , Jia, Yanyan , Liu, Yiwei , Qin, Hongpeng , Wang, Yibo , Jiang, Zhuqing . Imaging Characteristic Optimization of Digital Holographic microscopy for Onion Epidermal Cells . (2019) . |
导入链接 | NoteExpress RIS BibTex |
摘要 :
A noise reduction method based on a shorter synthetic-wavelength in DWDH is proposed in this paper. The unwrapped phase at longer synthetic-wavelength is calculated by using the wrapped phases of two individual wavelengths. By comparing the amplified phase of longer synthetic wavelength, of which magnification is equal to that of wavelengths with the wrapped phase of a single wavelength, the difference of phase noises between the longer synthetic-wavelength phase map and the single-wavelength phase map can be calculated, and then accurate height at single-wavelength can be achieved. The proposed method for calculating phase noise is performed by comparing the phases between the single-wavelength with the shorter synthetic wavelength, and then the height at shorter synthetic-wavelength can be obtained. Compared with the existing method, the proposed method can reduce certain noise and benefit the phase reconstruction of fine structures.
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GB/T 7714 | Huang, Meng , Wang, Yibo , Qin, Hongpeng et al. Noise reduction of dual-wavelength digital holography based on a shorter synthetic-wavelength [C] . 2019 . |
MLA | Huang, Meng et al. "Noise reduction of dual-wavelength digital holography based on a shorter synthetic-wavelength" . (2019) . |
APA | Huang, Meng , Wang, Yibo , Qin, Hongpeng , Jiang, Zhuqing . Noise reduction of dual-wavelength digital holography based on a shorter synthetic-wavelength . (2019) . |
导入链接 | NoteExpress RIS BibTex |
摘要 :
We introduce a new STDH modality to record and process hybrid space-time representations. This allows improving resolution with one single object scan. Onion epidermal cells has been used as samples to verify this approach. © OSA 2019 © 2019 The Author(s)
关键词 :
Holography Holography Imaging systems Imaging systems
引用:
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GB/T 7714 | Wang, Zhe , Bianco, Vittorio , Cui, Yutong et al. 2D resolution enhancement from 1D scanning Space-Time Digital Holography (STDH) [C] . 2019 . |
MLA | Wang, Zhe et al. "2D resolution enhancement from 1D scanning Space-Time Digital Holography (STDH)" . (2019) . |
APA | Wang, Zhe , Bianco, Vittorio , Cui, Yutong , Paturzo, Melania , Jiang, Zhuqing , Ferraro, Pietro . 2D resolution enhancement from 1D scanning Space-Time Digital Holography (STDH) . (2019) . |
导入链接 | NoteExpress RIS BibTex |
摘要 :
A measurement of microfluidic refractive index is presented by digital holography. Salt solutions of different concentrations are each injected within microfluidic chip, and their refractive indexes are obtained according to the digital holographic phase imaging. © 2018 The Author(s).
关键词 :
Three dimensional displays Three dimensional displays Holography Holography Image acquisition Image acquisition Microscopic examination Microscopic examination Digital microfluidics Digital microfluidics Refractive index Refractive index
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GB/T 7714 | Sun, Chan , Cui, Yutong , Wang, Zhe et al. Measurement of microfluidic refractive index by digital holographic microscopy [C] . 2018 . |
MLA | Sun, Chan et al. "Measurement of microfluidic refractive index by digital holographic microscopy" . (2018) . |
APA | Sun, Chan , Cui, Yutong , Wang, Zhe , Jiang, Zhuqing . Measurement of microfluidic refractive index by digital holographic microscopy . (2018) . |
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