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学者姓名:彭永臻
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Abstract :
This study proposed a novel strategy that integrates S-0 particles (diameter: 2-3 mm) and granular sludge to establish S-0-driven partial denitrification coupled with anammox (S(0)PDA) process for autotrophic nitrogen removal from NH4+- and NO3--containing wastewaters. This process was evaluated using an up-flow anoxic sludge bed bioreactor, operating continuously for 240 days. The influent concentrations of NH4+ and NO3- were 29.9 +/- 2.7 and 50.2 +/- 2.7 mg-N/L, respectively. Throughout the operation, the hydraulic retention time was shortened from 4.0 h to 2.0 h, while the effluent concentrations of NH (+) and NO3- were maintained at a desirable level of 1.45-1.51 mg-N/L and 4.46-6.52 mg-N/L, respectively. Despite an autotrophic process, the nitrogen removal efficiency and rate reached up to 88.5 +/- 2.0 % and 1.75 +/- 0.07 kg-N/(m(3).d), respectively, indicating the remarkable robustness of the S(0)PDA process. Autotrophic anammox and sulfur-oxidizing bacteria (Candidatus Brocadia and Thiobacillus) were the predominant bacterial genera involved in the S(0)PDA process. Candidatus Brocadia was primarily enriched in the granular sludge, with a relative abundance of 6.70 %. Thiobacillus occupied a unique niche on the S-0 particles, with a relative abundance as high as 57.6 %, of which Thiobacillus thioparus with partial denitrification function (reducing NO3- to NO2- without further reduction to N-2) accounted for 78.0 %. These findings challenge the stereotype of low efficiency in autotrophic nitrogen removal from wastewater, shedding fresh light on the applications of autotrophic processes.
Keyword :
Granular sludge Granular sludge S-0 particles S-0 particles Anammox Anammox Autotrophic nitrogen removal Autotrophic nitrogen removal S-0-driven partial denitrification S-0-driven partial denitrification
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GB/T 7714 | Wang, Luyao , Zhao, Qi , Zhang, Liang et al. S0-driven partial denitrification coupled with anammox (S0PDA) enables highly efficient autotrophic nitrogen removal from wastewater [J]. | WATER RESEARCH , 2024 , 255 . |
MLA | Wang, Luyao et al. "S0-driven partial denitrification coupled with anammox (S0PDA) enables highly efficient autotrophic nitrogen removal from wastewater" . | WATER RESEARCH 255 (2024) . |
APA | Wang, Luyao , Zhao, Qi , Zhang, Liang , Wu, Di , Zhou, Jiazhong , Peng, Yongzhen . S0-driven partial denitrification coupled with anammox (S0PDA) enables highly efficient autotrophic nitrogen removal from wastewater . | WATER RESEARCH , 2024 , 255 . |
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Abstract :
The exogenous hydroxylamine dosing has been proven to enhance nitrite supply for anammox bacteria. In this study, exogenous hydroxylamine was fed into a sequencing batch reactor to investigate its long-term effect on anammox granular sludge. The results showed that hydroxylamine enhanced the reactor's performance with an increase in total nitrogen removal rate from 0.23 to 0.52 kg N/m3/d and an increase in bacterial activity from 11.65 to 78.24 mg N/g VSS/h. Meanwhile, hydroxylamine promoted granulation by eluting flocs. And higher anammox activity and granulation were supported by extracellular polymeric substances (EPS) characteristics. Moreover, Candidatus Brocadia's abundance increased from 1.10 % to 3.03 %, and its symbiosis with heterotrophic bacteria was intensified. Additionally, molecular docking detailed the mechanism of the hydroxylamine effect. Overall, this study would provide new insights into the hydroxylamine dosing strategy application.
Keyword :
Granulation Granulation Bacterial activity Bacterial activity Anammox Anammox Hydroxylamine Hydroxylamine
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GB/T 7714 | Su, Xinwei , Li, Jianwei , Peng, Yi et al. An overlooked effect of hydroxylamine on anammox granular sludge: Promoting granulation and boosting activity [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2024 , 921 . |
MLA | Su, Xinwei et al. "An overlooked effect of hydroxylamine on anammox granular sludge: Promoting granulation and boosting activity" . | SCIENCE OF THE TOTAL ENVIRONMENT 921 (2024) . |
APA | Su, Xinwei , Li, Jianwei , Peng, Yi , Yuan, Yue , Wu, Lei , Peng, Yongzhen . An overlooked effect of hydroxylamine on anammox granular sludge: Promoting granulation and boosting activity . | SCIENCE OF THE TOTAL ENVIRONMENT , 2024 , 921 . |
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Realizing the quick enrichment and development of denitrifying phosphorus accumulating organisms (DPAOs) in actual household wastewater and industrial nitrate wastewater has significant research significance. In this study, a novel operation mode of anaerobic-oxic-anoxic (AOA) was adopted to successfully realize the enrichment and cultivation of DPAOs in urban domestic wastewater. Adjusting influent COD to PO43--P ratio, shortening the aerobic time and decreasing the aeration volume were conducive to select DPAOs in microbial populations. The system was operated for 180 days and the DPAOs were well enriched during the stable operation with the percentage of Dechloromonas increased to 5.1 %. Accordingly, the effluent PO43--P was < 0.3 mg P/L, the removal efficiency of phosphorus was 96.9 % and the removal efficiency of nitrate was 92.5 %. Above all, DPR can be successfully applied to AOA systems with good phosphorus removal performance.
Keyword :
microorganisms microorganisms Denitrifying phosphorus accumulating Denitrifying phosphorus accumulating AOA operation AOA operation Endogenous denitrification Endogenous denitrification Municipal wastewater Municipal wastewater Denitrifying phosphorus removal Denitrifying phosphorus removal
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GB/T 7714 | Li, Zixin , Li, Xiyao , Wang, Hanbin et al. Achieving synchronous and highly efficient removal of nitrogen and phosphorus by rapid enrichment and cultivation denitrifying phosphorus accumulating organisms in anaerobic-oxic-anoxic operation mode [J]. | BIORESOURCE TECHNOLOGY , 2024 , 396 . |
MLA | Li, Zixin et al. "Achieving synchronous and highly efficient removal of nitrogen and phosphorus by rapid enrichment and cultivation denitrifying phosphorus accumulating organisms in anaerobic-oxic-anoxic operation mode" . | BIORESOURCE TECHNOLOGY 396 (2024) . |
APA | Li, Zixin , Li, Xiyao , Wang, Hanbin , Peng, Yongzhen . Achieving synchronous and highly efficient removal of nitrogen and phosphorus by rapid enrichment and cultivation denitrifying phosphorus accumulating organisms in anaerobic-oxic-anoxic operation mode . | BIORESOURCE TECHNOLOGY , 2024 , 396 . |
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Abstract :
Partial nitrification (PN) has great significance in promoting both anaerobic ammonium oxidation (anammox) and advanced nitrogen removal. However, the kinetics of PN have not been sufficiently studied. Deterioration of PN often occurs due to nitrite-oxidizing bacteria (NOB) outgrowth, for which the dissolved oxygen (DO) is a critical factor. This study investigated the kinetic parameters of ammonia-oxidizing bacteria (AOB) and NOB by varying the DO concentration. The partial nitrification-anammox (PNA) process was operated under sequentially low (0.3-0.8 mg/L) and high (4.0-5.0 mg/L) DO. The results show that AOB could not adapt to low DO, resulting in a significant decrease in the maximum AOB activity (Vmax,AOB) and a slightly altered oxygen half-saturation constant of AOB (KO,AOB). Conversely, KO,NOB decreased and Vmax,NOB maintained, indicating that the main NOB genus, Nitrospira, was more adaptable to DO changes. Under high DO, AOB growth was weaker than NOB, leading to a low Vmax,AOB - Vmax,NOB value; KO,AOB increased and was larger than KO,NOB and PNA was eventually destroyed. The aerobic starvation was then adopted to restore PN. AOB recovered faster and dominated, with Vmax,AOB greatly exceeding Vmax,NOB. Finally, NOB were inhibited and PN was recovered. During this process, KO, NOB increased and was higher than KO,AOB. Changes in the kinetic parameters, especially the Vmax,AOB -Vmax,NOB value, have specific guiding significance for DO regulation during PNA. This work helps better understand the effect of DO variations on the nitrifying microbial community, which influences oxygen affinity and maximum activity.
Keyword :
Dissolved oxygen Dissolved oxygen Oxygen affinity Oxygen affinity Partial nitrification Partial nitrification Nitrifying microbial community Nitrifying microbial community Maximum nitrification activity Maximum nitrification activity
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GB/T 7714 | Duan, Chenxue , Zhang, Qiong , Li, Jialin et al. Partial nitrification response to dissolved oxygen variation and aerobic starvation: Kinetics and microbial community analyses [J]. | CHEMICAL ENGINEERING JOURNAL , 2024 , 481 . |
MLA | Duan, Chenxue et al. "Partial nitrification response to dissolved oxygen variation and aerobic starvation: Kinetics and microbial community analyses" . | CHEMICAL ENGINEERING JOURNAL 481 (2024) . |
APA | Duan, Chenxue , Zhang, Qiong , Li, Jialin , Feng, Wanyi , Zhang, Liang , Peng, Yongzhen . Partial nitrification response to dissolved oxygen variation and aerobic starvation: Kinetics and microbial community analyses . | CHEMICAL ENGINEERING JOURNAL , 2024 , 481 . |
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Abstract :
Anaerobic ammonia oxidation (anammox) of municipal wastewater is a research focus, especially the combined treatment with mature landfill leachate is a current research hotspot. In this study, municipal wastewater was treated by partial nitrification via sequencing batch reactor (SBR), and its effluent and mature landfill leachate were then mixed into an up-flow anaerobic sludge blanket (UASB) for simultaneous anammox and partial denitrification reaction. Through partial nitrification, a high nitrite accumulation rate (93.0 +/- 3.8 %) was achieved by low dissolved oxygen (0.5-1.6 mg/L) and controlled aerobic time (3.5 h) in SBR. The UASB system was responsible for 78.8 +/- 2.1 % nitrogen removal of the entire system with a hydraulic reaction time (HRT) of 3.8 h, accompanied by the anammox contribution up to 89.4 +/- 6.0 %. The overall partial nitrificationsimultaneous anammox and partial denitrification (PN-SAPD) system was controlled at a total COD/TIN of 2.8 +/- 0.3 and a total HRT of only 10.2 h, achieving the nitrogen removal efficiency and effluent TIN were 95.2 +/- 2.2 % and 3.4 +/- 1.5 mg/L, respectively. The qPCR results showed functional genes (hzsA(B), hdh) associated with anaerobic ammonia-oxidizing bacteria (AnAOB), whose high gene copy abundance and transcription expression ensured the removal of major nitrogen from municipal wastewater and mature landfill leachate. 16S amplicon sequencing showed that the Ca. Brocadia (9.72-12.6 %) was further enrichment after sodium acetate was added, and the transcription expression of Thauera (0.5-7.0 %) caused nitrate to nitrite. The high abundance of related enzymes (hao, hzs, hdh, narGHI) involved in anammox and partial denitrification processes were found in the macrogenomic sequencing, and only Ca. Brocadia was involved in multi-pathway nitrogen metabolism in AnAOB. Based on the efficient nitrogen removal by AnAOB and denitrifying bacteria, this modified PN-SAPD process provides a new option for the co-treatment of mature landfill leachate in municipal wastewater treatment plants.
Keyword :
Anammox Anammox Municipal wastewater Municipal wastewater Landfill leachate Landfill leachate Partial nitrification Partial nitrification Nitrogen metabolism Nitrogen metabolism Biological nitrogen removal Biological nitrogen removal
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GB/T 7714 | Xiong, Lulu , Li, Xiyao , Li, Jianwei et al. Efficient nitrogen removal from real municipal wastewater and mature landfill leachate using partial nitrification-simultaneous anammox and partial denitrification process [J]. | WATER RESEARCH , 2024 , 251 . |
MLA | Xiong, Lulu et al. "Efficient nitrogen removal from real municipal wastewater and mature landfill leachate using partial nitrification-simultaneous anammox and partial denitrification process" . | WATER RESEARCH 251 (2024) . |
APA | Xiong, Lulu , Li, Xiyao , Li, Jianwei , Zhang, Qiong , Zhang, Liang , Wu, You et al. Efficient nitrogen removal from real municipal wastewater and mature landfill leachate using partial nitrification-simultaneous anammox and partial denitrification process . | WATER RESEARCH , 2024 , 251 . |
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Low water temperatures and ammonium concentrations pose challenges for anammox applications in the treatment of low C/N municipal wastewater. In this study, a 10 L -water bath sequencing batch reactor combing biofilm and suspended sludge was designed for low C/N municipal wastewater treatment. The nitrogen removal performance via partial nitrification anammox-(endogenous) denitrification anammox process was investigated with anaerobic -aerobic -anoxic mode at low temperatures and dissolved oxygen (DO). The results showed that with the decrease of temperature from 30 to 15 degrees C, the influent and effluent nitrogen concentrations and nitrogen removal efficiencies were 73.7 +/- 6.5 mg/L, 7.8 +/- 2.8 mg/L, and 89.4 %, respectively, with aerobic hydraulic retention time of only 6 h and DO concentration of 0.2-0.5 mg/L. Among that, the stable anammox process compensated for the inhibitory effects of the low temperatures on the nitrification and denitrification processes. Notably, from 30 to 15 degrees C, the anammox activity and relative abundance of the dominant Brocadia genus were increased from 39.7 to 45.5 mgN/gVSS/d and 7.3 to 12.0 %, respectively; the single gene expression level of the biofilm increased 9.0 times. The anammox bacteria showed a good adaptation to temperatures reduction. However, nitrogen removal by anammox was not improved by increasing DO (>= 4 mg/L) at 8-4 degrees C. Overall, the results of this study demonstrate the feasibility of the mainstream anammox process at low temperatures.
Keyword :
Low temperature Low temperature Anammox Anammox Low C/N municipal wastewater Low C/N municipal wastewater Hybrid treatment system Hybrid treatment system Dissolved oxygen regulation Dissolved oxygen regulation
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GB/T 7714 | Wang, Rui , Liu, Jinjin , Zhang, Qiong et al. Robustness of the anammox process at low temperatures and low dissolved oxygen for low C/N municipal wastewater treatment [J]. | WATER RESEARCH , 2024 , 252 . |
MLA | Wang, Rui et al. "Robustness of the anammox process at low temperatures and low dissolved oxygen for low C/N municipal wastewater treatment" . | WATER RESEARCH 252 (2024) . |
APA | Wang, Rui , Liu, Jinjin , Zhang, Qiong , Li, Xiyao , Wang, Shuying , Peng, Yongzhen . Robustness of the anammox process at low temperatures and low dissolved oxygen for low C/N municipal wastewater treatment . | WATER RESEARCH , 2024 , 252 . |
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Abstract :
Aerobic granular sludge (AGS) capable of nitrogen and phosphorus removal is mainly limited to the applications in sequencing batch reactors. This study introduced an innovative continuous self-circulating up-flow fluidized bed process (Zier process) using separate aeration. The process was composed of an anoxic column (Zier-A), aeration column (Zier-OO) and aerobic column (Zier-O), and was used to treat actual municipal sewage continuously for 170 days. The process achieved self-circulation of 20-32 times and an up-flow velocity within the reactor of 7-16 m/h which were accurately controlled with only separate aeration. The larger proportion of self-circulating multiple times contributed to particle formation and stability, providing hydraulic shear conditions, and screened the precipitation performance of the granular sludge (GS). Meanwhile, the dissolved oxygen (DO) of Zier-O was controlled at 0.1-0.3 mg/L, and the DO of Zier-A input water was zero. The accurate oxygen supply enhanced simultaneous nitrification and denitrification (SND) as well as short-cut nitrification and denitrification in Zier-O and improved the COD utilization rate and the nitrogen removal rate in Zier-A. The COD treatment capacity reached 2.46 kg-COD/(m(3)d). With a hydraulic retention time of 10 h, the process consistently ensured that the average concentrations of ammonia nitrogen and total nitrogen in the effluent were maintained below 5 and 15 mg/L, respectively. Moreover, the process maintained the shape and stability of GS, the median diameter of GS ranged between 300-1210 mu m, the percentage of mass with particle size distribution < 200 mu m at a height of 150 cm within Zier-A and Zier-O accounted for as low as 0.04%-0.05%, and showed good settling performance. The suspended solids in effluent can be maintained at 50-80 mg/L. Overall, the unique structural setting and control method of the Zier process provide another approach for the application of continuous AGS treatment for municipal sewage.
Keyword :
Municipal sewage Municipal sewage Biological nitrogen removal Biological nitrogen removal Aerobic granular sludge Aerobic granular sludge Up-flow sludge fluidized bed Up-flow sludge fluidized bed
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GB/T 7714 | Wang, Cong , Qi, Wei-Kang , Zhang, Shu-Jun et al. Innovation for continuous aerobic granular sludge process in actual municipal sewage treatment: Self-circulating up-flow fluidized bed process [J]. | WATER RESEARCH , 2024 , 260 . |
MLA | Wang, Cong et al. "Innovation for continuous aerobic granular sludge process in actual municipal sewage treatment: Self-circulating up-flow fluidized bed process" . | WATER RESEARCH 260 (2024) . |
APA | Wang, Cong , Qi, Wei-Kang , Zhang, Shu-Jun , Liu, Li-Fang , Peng, Yong-Zhen . Innovation for continuous aerobic granular sludge process in actual municipal sewage treatment: Self-circulating up-flow fluidized bed process . | WATER RESEARCH , 2024 , 260 . |
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Currently, partial nitrification (PN) processes are commonly used to improve efficiency in high ammonia nitrogen wastewaters. However, initiating and restoring damaged PN performance is crucial for optimizing nitrogen removal efficiency without the use of chemical additives. In this study, a simultaneous partial nitrification and denitrification coupled with anammox (SPNAD) sequencing batch biofilm reactor was constructed to treat anaerobic digestion after sludge thermal hydrolysis pretreatment (STHP-AD) filtrate. The PN performance was successfully recovered through optimizing the operation mode of anoxic/oxic alternation within 120 days. Result showed that Nitrosomonas increased from 0.68 % to 3.43 %, while Nitrospira decreased to 0.06 %. The PN recovery also promoted anammox, with the only anammox bacterium of Candidatus Brocadia, detected in the mixing and biofilm sludge of 1.73 % and 1.41 %, respectively. The autotrophic denitrifying bacteria Thermomonas (increasing from 0.47 % to 23.85 %) and Truepera (rising from 2.31 % to 6.54 %) rapidly accumulated. After adjusting to the anoxic/oxic alternation ratio, the hydraulic retention time was shortened from 50 h to 37.5 h, and the oxic time was reduced to half of the initial operation. The PN recovery and anammox enrichment resulted in improving nitrogen removal approximately 20 %. Eventually, the SPNAD system realizes stable and efficient nitrogen removal with the influent nitrogen loading rate of 0.43 kgN/(m3 & sdot;d), the ammonium removal efficiency of 94.73 +/- 3.27 %, and the nitrogen removal efficiency of 72.98 +/- 1.27 %. In this study, a new strategy using intermittent aeration while appropriately extending the oxic time was proposed to restore the system's ability to treat STHP-AD filtrate after PN disruption.
Keyword :
STHP-AD filtrate STHP-AD filtrate Sequencing batch biofilm reactor (SBBR) Sequencing batch biofilm reactor (SBBR) Intermittent aeration Intermittent aeration Anammox Anammox Partial nitrification (PN) Partial nitrification (PN)
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GB/T 7714 | Zhang, Qianyu , Zhang, Qiong , Li, Jialin et al. Optimizing anoxic/oxic intervals in intermittent aeration for STHP-AD filtrate treatment: Restoring partial nitrification and community dynamics in the SPNAD system [J]. | CHEMICAL ENGINEERING JOURNAL , 2024 , 501 . |
MLA | Zhang, Qianyu et al. "Optimizing anoxic/oxic intervals in intermittent aeration for STHP-AD filtrate treatment: Restoring partial nitrification and community dynamics in the SPNAD system" . | CHEMICAL ENGINEERING JOURNAL 501 (2024) . |
APA | Zhang, Qianyu , Zhang, Qiong , Li, Jialin , Peng, Yongzhen . Optimizing anoxic/oxic intervals in intermittent aeration for STHP-AD filtrate treatment: Restoring partial nitrification and community dynamics in the SPNAD system . | CHEMICAL ENGINEERING JOURNAL , 2024 , 501 . |
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Partial nitrification (PN) represents an energy-efficient bioprocess; however, it often confronts challenges such as unstable nitrite accumulation, nitrite oxidizing bacteria shocks, and slow reaction rate. This study established an acidophilic PN with self-sustained pH as low as 5.36. Over 120-day monitoring, nitrite accumulation rate (NAR) was stabilized at more than 97.9 %, and an ultra-high ammonia oxidation rate of 0.81 kg/m(3)& sdot;d was achieved. Notably, least NAR of 77.8 % persisted even under accidental nitrite oxidizing bacteria invasion, aeration delay, alkalinity fluctuations, and cooling shocks. During processing, despite detrimental effects on bacterial diversity, there was a discernible increase in acid-tolerant bacteria responsible for nitrification. Candidatus Nitrosoglobus, gradually dominated in nitrifying guild (2.15 %), with the substantially reduction or disappearance of typical nitrifying microorganisms. Acidobacteriota, a key player in nitrogen cycling of soil, significantly increased from 0.45 % to 9.98 %, and its associated nitrogen metabolism genes showed a substantial 215 % rise. AmoB emerged as the most critical functional gene influencing acidophilic PN, exhibiting significantly higher unit gene expression than other nitrification genes. Blockade tricarboxylic acid cycle, DNA damage, and presence of free nitrous acid exert substantial effects on nitrite-oxidizing bacteria (NOB), serving as internal driving forces for acidophilic PN. This highlights the reliable potential for shortening nitrogen transformation process.
Keyword :
Ultra -fast reaction rate Ultra -fast reaction rate Candidatus nitrosoglobus Candidatus nitrosoglobus Acidophilic partial nitrification Acidophilic partial nitrification pH < 6 pH < 6 Acidobacteriota Acidobacteriota
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GB/T 7714 | Zhang, Fangzhai , Du, Ziyi , Wang, Jiahui et al. Acidophilic partial nitrification (pH<6) facilitates ultra-efficient short-flow nitrogen transformation: Experimental validation and genomic insights [J]. | WATER RESEARCH , 2024 , 260 . |
MLA | Zhang, Fangzhai et al. "Acidophilic partial nitrification (pH<6) facilitates ultra-efficient short-flow nitrogen transformation: Experimental validation and genomic insights" . | WATER RESEARCH 260 (2024) . |
APA | Zhang, Fangzhai , Du, Ziyi , Wang, Jiahui , Du, Yujia , Peng, Yongzhen . Acidophilic partial nitrification (pH<6) facilitates ultra-efficient short-flow nitrogen transformation: Experimental validation and genomic insights . | WATER RESEARCH , 2024 , 260 . |
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Controlling sludge concentration is an effective means to achieve PN. In this article, the reactor used domestic sewage as raw water and promoted the high enrichment of anammox bacteria by controlling the MLVSS of flocs to 1000-1500 mg/L and increasing the concentration of filler sludge. The measures to reduce the concentration of flocculent sludge increased the proliferation rate of the biofilm and provided sufficient substrate for AnAOB. After 102 days of operation, the abundance of Candidatus Brocadia increased from 0.43% during inoculation to 23.56% in phase VI. The ability of the microbial community to utilize energy metabolism and produce ATP was significantly improved, and the appropriate distribution of anammox bacteria and nitrifying, denitrifying bacteria in the ecological niche led to its high enrichment. In summary, this study proposes a strategy to promote the high enrichment of anammox bacteria in mainstream domestic sewage without adding any chemicals.
Keyword :
Partial nitrification (PN) Partial nitrification (PN) Anammox bacteria enrichment Anammox bacteria enrichment Control sludge concentration Control sludge concentration Domestic sewage Domestic sewage
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GB/T 7714 | Han, Xueke , Liu, Jinjin , Zhu, Zhuo et al. Strengthening the enrichment of anaerobic ammonia oxidizing bacteria in biofilms through sludge concentration control [J]. | ENVIRONMENTAL RESEARCH , 2024 , 262 . |
MLA | Han, Xueke et al. "Strengthening the enrichment of anaerobic ammonia oxidizing bacteria in biofilms through sludge concentration control" . | ENVIRONMENTAL RESEARCH 262 (2024) . |
APA | Han, Xueke , Liu, Jinjin , Zhu, Zhuo , Lin, Yangang , Peng, Yongzhen . Strengthening the enrichment of anaerobic ammonia oxidizing bacteria in biofilms through sludge concentration control . | ENVIRONMENTAL RESEARCH , 2024 , 262 . |
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