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摘要 ：
The significance of inorganic carbon (IC) for anaerobic ammonium oxidation (anammox) bacteria has been verified. However, the regulation of metabolic pathways under IC stress is not clear, limiting the optimization of IC supply. In this study, the regulatory pathways at IC concentration of 5-150 mg/L were explored to achieve optimal control of IC. The results show that the changes of metabolic pathway under IC stress determined anammox characteristics. At IC concentration of 5 mg/L, the anammox activity distinctly decreased due to the guanosine tetraphosphate (ppGpp) -mediated regulation under IC limitation. With less than 15 mg/L of IC, the decrease of carbon fixation limited the biosynthesis of gluconeogenesis and amino acids, causing the decline of extracellular polymeric substance synthesis. With more than 50 mg/L of IC, the improvement of purine and pyrimidine metabolism enhanced the electron transport capacity and growth potential of anammox bacteria. This study provides metabolic insights into IC influence on anammox consortia and a novel method of IC concentration optimization using metabolomics analysis.
关键词 ：
Metabolomics Metabolomics Inorganic carbon Inorganic carbon Anaerobic ammonium oxidation Anaerobic ammonium oxidation Extracellular polymeric substance (EPS) Extracellular polymeric substance (EPS)
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| GB/T 7714 | Ma, Chenyang , Zeng, Wei , Li, Jianmin et al. Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 855 . |
| MLA | Ma, Chenyang et al. "Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process" . | SCIENCE OF THE TOTAL ENVIRONMENT 855 (2023) . |
| APA | Ma, Chenyang , Zeng, Wei , Li, Jianmin , Meng, Qingan , Peng, Yongzhen . Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process . | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 855 . |
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摘要 ：
一种检测活性污泥系统中活性功能菌的方法，属于污水生物处理技术领域。本发明利用改良的生物正交氨基酸标记染色法联合流式细胞仪分选技术和测序技术对污水处理系统中功能活性微生物的种群占比。通过改良的生物正交氨基酸标记染色法对活性功能菌进行特异性染色，在利用流式细胞仪识别并分选出活性功能细菌种群；分选后的细菌进行预处理后，根据改良的微量DNA试剂盒对样品进行预处理，进而利用苯酚抽提法提取DNA，并对提取后的DNA进行16SrRNA高通量测序；根据分选前和分选后的高通量测序结果进行比对，得出活性功能菌的种群丰度。本发明提供了一种经济、简便、可靠的污水处理系统活性污泥中活性功能菌的定量方法。
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| GB/T 7714 | 曾薇 , 孟庆安 , 刘宏军 . 一种检测活性污泥系统中活性功能菌的方法 : CN202310608738.5[P]. | 2023-05-29 . |
| MLA | 曾薇 et al. "一种检测活性污泥系统中活性功能菌的方法" : CN202310608738.5. | 2023-05-29 . |
| APA | 曾薇 , 孟庆安 , 刘宏军 . 一种检测活性污泥系统中活性功能菌的方法 : CN202310608738.5. | 2023-05-29 . |
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摘要 ：
一种生物正交氨基酸标记染色法联合荧光原位杂交检测活性污泥系统中活性聚磷菌的方法，属于污水生物处理技术领域。本发明使用L‑高炔丙基甘氨酸培养活性污泥中微生物，并通过生物正交氨基酸标记染色法对利用该氨基酸合成蛋白质的微生物进行荧光染色；采用荧光原位杂交技术使探针PAO846、PAO651、PAO462与荧光染色后的样品中聚磷菌目的基因进行杂交；利用流式细胞仪检测被荧光标记的细菌，通过圈门处理识别出活性聚磷菌区域，进而计算和分析活性聚磷菌占全菌的比例。本发明提供了一种经济、简便、可靠的污水处理系统活性污泥中活性聚磷菌的定量方法，为实际污水处理厂调控生物除磷条件提供了理论依据。
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| GB/T 7714 | 曾薇 , 孟庆安 , 刘宏军 et al. 一种生物正交氨基酸标记染色法联合荧光原位杂交技术检测活性污泥系统中活性聚磷菌的方法 : CN202310164818.6[P]. | 2023-02-26 . |
| MLA | 曾薇 et al. "一种生物正交氨基酸标记染色法联合荧光原位杂交技术检测活性污泥系统中活性聚磷菌的方法" : CN202310164818.6. | 2023-02-26 . |
| APA | 曾薇 , 孟庆安 , 刘宏军 , 彭永臻 . 一种生物正交氨基酸标记染色法联合荧光原位杂交技术检测活性污泥系统中活性聚磷菌的方法 : CN202310164818.6. | 2023-02-26 . |
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摘要 ：
一种基于Fe2+和Fe3+循环驱动的污水生物脱氮方法，属于污水生物处理领域。本发明以城市污水处理厂二沉池活性污泥为种泥成功富集了厌氧铁氨氧化和硝酸盐依赖型Fe2+氧化污泥，实现了二者耦合一体化脱氮。仅在反应器运行第一天投加1g/L Fe2O3，通过Fe2+和Fe3+循环实现了长期稳定高效脱氮，氨氮和总氮去除率最高达100％和92.45％。NH4+‑N在厌氧铁氨氧化作用下生成N2和NO3‑‑N，Fe3+被还原为Fe2+。生成的Fe2+通过两种途径氧化为Fe3+，一种途径是微生物介导的硝酸盐依赖型Fe2+氧化，另一种途径是被进水溶解氧化学氧化为Fe3+。本发明无需去氧、无需曝气和外加有机物，且利用厌氧铁氨氧化和硝酸盐依赖型Fe2+氧化分别消耗H+和产生H+的特性，使出水pH值稳定在7‑8，实现高效稳定脱氮。
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| GB/T 7714 | 曾薇 , 郝小静 , 李健敏 et al. 一种基于Fe2+和Fe3+循环驱动的污水生物脱氮方法 : CN202310650342.7[P]. | 2023-06-03 . |
| MLA | 曾薇 et al. "一种基于Fe2+和Fe3+循环驱动的污水生物脱氮方法" : CN202310650342.7. | 2023-06-03 . |
| APA | 曾薇 , 郝小静 , 李健敏 , 詹孟佳 , 苗豪豪 , 宫庆腾 . 一种基于Fe2+和Fe3+循环驱动的污水生物脱氮方法 : CN202310650342.7. | 2023-06-03 . |
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摘要 ：
本发明公开了一种利用亚硝酸盐快速培养发酵型反硝化聚磷菌的方法。该方法以酪蛋白胨模拟生活污水/剩余污泥中的大分子有机物作为唯一碳源。首先在第一阶段，经过厌氧/好氧模式连续运行60天以上后出水磷酸盐浓度稳定达到0.5mg/L以下，指示发酵型聚磷菌富集完成。之后在第二阶段取第一阶段反应器中的污泥接种到新的序批式反应器中，增加缺氧段并投加亚硝酸盐氮浓度为10～20mg/L以构建反硝化除磷系统，经过10～15d运行后出水磷酸盐和亚硝酸盐浓度分别小于0.5mg/L和1.0mg/L并能稳定维持，指示发酵型反硝化聚磷菌驯化完成。本发明利用酪蛋白胨作为碳源，通过投加亚硝酸盐快速实现了发酵型反硝化聚磷菌的培养，稳定实现低C/N生活污水高效同步脱氮除磷。
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| GB/T 7714 | 曾薇 , 张佳玉 , 孟庆安 et al. 一种利用亚硝酸盐快速培养发酵型反硝化聚磷菌的方法 : CN202310882246.5[P]. | 2023-07-18 . |
| MLA | 曾薇 et al. "一种利用亚硝酸盐快速培养发酵型反硝化聚磷菌的方法" : CN202310882246.5. | 2023-07-18 . |
| APA | 曾薇 , 张佳玉 , 孟庆安 , 刘宏军 . 一种利用亚硝酸盐快速培养发酵型反硝化聚磷菌的方法 : CN202310882246.5. | 2023-07-18 . |
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摘要 ：
Titanium salt coagulant, as a new type of water treatment agent, has been widely studied, but most researches do not consider its effect on the biological treatment. In this study, different doses of TiCl4 were added to the biological phosphorus removal (BPR) system to investigate the impact of TiCl4 on BPR. The results showed that the addition of TiCl4 not only significantly reduced the phosphorus concentration in effluent (below 0.5 mg/L), but also kept it stable. Moreover, the sedimentation performance of activated sludge was improved, which was superior to the control group. According to the results of flow cytometry (FCM), a small amount of TiCl4 significantly improved the bioactivities, but excessive dosage caused inhibition. When the dosage of TiCl4 below 20 mg/L, polyphosphate accumulating metabolism (PAM) was strengthened. In addition, the richness of microbial community and the relative abundance of Candidatus Accumulibacter clades also increased. However, when the dosage reached 60 mg/L, the relative abundance of Candidatus Competibacter increased and the BPR system was deteriorated. This study suggests that the addition of appropriate concentration of TiCl4 can realize the synergistic enhancement of biological and chemical phosphorus removal in sewage treatment.
关键词 ：
Bacterial activity Bacterial activity Microbial community Microbial community TiCl4 TiCl4 Simultaneous chemical phosphorus removal (SCPR) Simultaneous chemical phosphorus removal (SCPR)
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| GB/T 7714 | Jia, Yuan , Zeng, Wei , Fan, Zhiwei et al. An effective titanium salt dosing strategy for phosphorus removal from wastewater: Synergistic enhancement of chemical and biological treatment [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
| MLA | Jia, Yuan et al. "An effective titanium salt dosing strategy for phosphorus removal from wastewater: Synergistic enhancement of chemical and biological treatment" . | SCIENCE OF THE TOTAL ENVIRONMENT 842 (2022) . |
| APA | Jia, Yuan , Zeng, Wei , Fan, Zhiwei , Meng, Qingan , Liu, Hongjun , Peng, Yongzhen . An effective titanium salt dosing strategy for phosphorus removal from wastewater: Synergistic enhancement of chemical and biological treatment . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
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摘要 ：
Tetrasphaera are polyphosphate accumulating organisms (PAOs) that play an important role in enhanced biological phosphorus removal (EBPR) from wastewater. The effect of a wide range of temperature changes (1-30 degrees C) on phosphorus removal, metabolism and clade-level community structure of Tetrasphaera-dominated PAOs was investigated. At 10 degrees C, the bioactivities of Tetrasphaera-dominated communities were obviously inhibited and the EBPR efficiency was only 73 %. Yet at 20-30 degrees C, EBPR efficiency reached 99 % and the relative abundance of Tetrasphaera was up to 90 %. The temperature variation changed the community distribution of Tetrasphaera clades, which was possibly a main reason for EBPR performance. Amino acids and PHA with different contents were intracellular metabolite of Tetrasphaera-dominated communities during phosphorus release and uptake at different temperatures. Moreover, Tetrasphaera fermented protein and amino acids and released VFAs. The outcomes suggested the great potential of Tetrasphaera-PAOs in the treatment of wastewater with varying temperatures and limited carbon sources.
关键词 ：
Tetrasphaera Tetrasphaera FISH FISH Temperatures Temperatures Polyphosphate accumulating organisms (PAOs) Polyphosphate accumulating organisms (PAOs) flow cytometry (FCM) flow cytometry (FCM) Enhanced biological phosphorus removal (EBPR) Enhanced biological phosphorus removal (EBPR)
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| GB/T 7714 | Liu, Hongjun , Zeng, Wei , Meng, Qingan et al. Phosphorus removal performance, intracellular metabolites and clade-level community structure of Tetrasphaera-dominated polyphosphate accumulating organisms at different temperatures [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
| MLA | Liu, Hongjun et al. "Phosphorus removal performance, intracellular metabolites and clade-level community structure of Tetrasphaera-dominated polyphosphate accumulating organisms at different temperatures" . | SCIENCE OF THE TOTAL ENVIRONMENT 842 (2022) . |
| APA | Liu, Hongjun , Zeng, Wei , Meng, Qingan , Fan, Zhiwei , Peng, Yongzhen . Phosphorus removal performance, intracellular metabolites and clade-level community structure of Tetrasphaera-dominated polyphosphate accumulating organisms at different temperatures . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
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摘要 ：
Simultaneous partial nitrification, biological phosphorous removal and sludge fermentation in continuous-flow system faced many challenges in wastewater treatment plants (WWTPs). In this study, a novel process was developed to achieve partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system dominated by Tetrasphaera treating low carbon/nitrogen ratio (COD/N) real domestic wastewater. After 80 days of operation, no external carbon was added into the influent. Nitrite accumulation ratio (NAR) reached 99.4% and removal efficiency of PO43- -P was 100%. The effluent concentration of total inorganic nitrogen (TIN) was lower than 2 mg/L. Compared with traditional biological nitrogen and phosphorus removal processes, the daily sludge discharge in continuous-flow PNPRF decreased by 61.9%. DNA concentration of the sludge supernatant after anaerobic zone was 2.26-fold of that before anaerobic zone, and percentages of intact cells decreased by 6.9% and necrotic cells increased by 8.3%, indicating that cell lysis caused the reduction of sludge discharge. The negative effect of prolonged anaerobic phase on transcriptional response of nxrB gene was more significant than that of amoA and hao gene, which resulted in stable partial nitrification. The activities of ammonium-oxidizing bacteria (AOB) were higher than that of nitrite-oxidizing bacteria (NOB), and reverse transcriptional PCR (RT-PCR) showed that RNA expression of AOB was higher than that of NOB. Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation in continuous-flow system dominated by Tetrasphaera solved the problems of carbon source deficiency and large amount of sludge discharge in treating real domestic wastewater process.
关键词 ：
Transcriptional level Transcriptional level Partial nitrification Partial nitrification Enhanced biological phosphorus removal Enhanced biological phosphorus removal Real domestic wastewater Real domestic wastewater Continuous flow Continuous flow
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| GB/T 7714 | Fan, Zhiwei , Zeng, Wei , Meng, Qingan et al. Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system and mechanism analysis at transcriptional level [J]. | CHEMICAL ENGINEERING JOURNAL , 2022 , 428 . |
| MLA | Fan, Zhiwei et al. "Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system and mechanism analysis at transcriptional level" . | CHEMICAL ENGINEERING JOURNAL 428 (2022) . |
| APA | Fan, Zhiwei , Zeng, Wei , Meng, Qingan , Liu, Hong , Ma, Chenyang , Peng, Yongzhen . Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system and mechanism analysis at transcriptional level . | CHEMICAL ENGINEERING JOURNAL , 2022 , 428 . |
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摘要 ：
Anaerobic ammonia oxidation (Anammox) coupling sulfur autotrophic denitrification is an effective method for the advanced nitrogen removal from the wastewater with limited carbon source. The influence of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification was investigated by adding different concentrations of S2O32--S (0, 39, 78, 156 and 312 mg/L) to the Anammox system. The contribution of sulfur autotrophic denitrification and Anammox to nitrogen removal at S2O32--S concentrations of 156 mg/L was 75% similar to 83% and 17%similar to 25%, respectively, and the mixed system achieved completely nitrogen removal. However, Anammox bioactivity was completely inhibited at S2O32--S concentrations up to 312 mg/L, and only sulfur autotrophic denitrification occurred. The isotopic effects of NO2--N (delta N-15(NO2) and delta O-18(NO2)) and NO3--N (delta N-15(NO3) and delta O-18(NO3)) during Anammox coupling sulfur autotrophic denitrification showed a gradual decrease trend with the increase of S2O32--S addition. The ratios of delta N-15(NO2):delta O-18(NO2) and delta N-15(NO3):delta O-18(NO3) was maintained at 1.30-2.41 and 1.36-2.52, respectively, which revealed that Anammox was dominant nitrogen removal pathway at S2O32--S concentrations less than 156 mg/L. Microbial diversity gradually decreased with the increase of S2O32--S. The S2O32--S addition enhanced the S2O32--driven autotrophic denitrification and weakened the Anammox, leading to a gradually decreasing trend of the proportion of Candidatus Brocadia as Anammox bacteria from the initial 27% to 4% (S2O32--S of 156 mg/L). Yet Norank-f-Hydrogenophilaceae (more than 50%) and Thiobacillus (54%) as functional bacteria of autotrophic denitrification obviously increased. The appropriate amount of S2O32--S addition promoted the performance of Anammox coupling sulfur autotrophic denitrification achieved completely nitrogen removal.
关键词 ：
S2O32--driven autotrophic denitrification S2O32--driven autotrophic denitrification Isotope effect Isotope effect Anammox Anammox Complete nitrogen removal Complete nitrogen removal Microbial diversity Microbial diversity
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| GB/T 7714 | Liu, Hong , Zeng, Wei , Li, Jianmin et al. Effect of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects [J]. | WATER RESEARCH , 2022 , 218 . |
| MLA | Liu, Hong et al. "Effect of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects" . | WATER RESEARCH 218 (2022) . |
| APA | Liu, Hong , Zeng, Wei , Li, Jianmin , Zhan, Mengjia , Fan, Zhiwei , Peng, Yongzhen . Effect of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects . | WATER RESEARCH , 2022 , 218 . |
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摘要 ：
一种基于海绵铁‑硫代硫酸钠自养反硝化生物滤池同步脱氮除磷的方法，属于污水生物处理领域。将火山岩和海绵体填料按照体积比5 : 1混合均匀后装填入反应器中，向反应器中接种硫自养反硝化污泥，控制进水S2O32‑和NO3‑‑N摩尔比为0.86，经过富集培养逐渐形成硫‑铁自养菌协同反硝化脱氮生物滤池。本发明的生物滤池构造简单、挂膜时间短，并且控制水力停留时间在1.5～3h就能够实现废水中硝酸盐和磷酸盐的高效同步去除，同时缓冲了pH和减少了硫酸盐的产生。
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| GB/T 7714 | 曾薇 , 苗豪豪 , 李健敏 et al. 一种基于海绵铁-硫代硫酸钠自养反硝化生物滤池同步脱氮除磷的方法 : CN202210860488.X[P]. | 2022-07-21 . |
| MLA | 曾薇 et al. "一种基于海绵铁-硫代硫酸钠自养反硝化生物滤池同步脱氮除磷的方法" : CN202210860488.X. | 2022-07-21 . |
| APA | 曾薇 , 苗豪豪 , 李健敏 , 彭永臻 . 一种基于海绵铁-硫代硫酸钠自养反硝化生物滤池同步脱氮除磷的方法 : CN202210860488.X. | 2022-07-21 . |
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