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摘要 ：
The significance of inorganic carbon (IC) for anaerobic ammonium oxidation (anammox) bacteria has been verified. However, the regulation of metabolic pathways under IC stress is not clear, limiting the optimization of IC supply. In this study, the regulatory pathways at IC concentration of 5-150 mg/L were explored to achieve optimal control of IC. The results show that the changes of metabolic pathway under IC stress determined anammox characteristics. At IC concentration of 5 mg/L, the anammox activity distinctly decreased due to the guanosine tetraphosphate (ppGpp) -mediated regulation under IC limitation. With less than 15 mg/L of IC, the decrease of carbon fixation limited the biosynthesis of gluconeogenesis and amino acids, causing the decline of extracellular polymeric substance synthesis. With more than 50 mg/L of IC, the improvement of purine and pyrimidine metabolism enhanced the electron transport capacity and growth potential of anammox bacteria. This study provides metabolic insights into IC influence on anammox consortia and a novel method of IC concentration optimization using metabolomics analysis.
关键词 ：
Metabolomics Metabolomics Inorganic carbon Inorganic carbon Anaerobic ammonium oxidation Anaerobic ammonium oxidation Extracellular polymeric substance (EPS) Extracellular polymeric substance (EPS)
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| GB/T 7714 | Ma, Chenyang , Zeng, Wei , Li, Jianmin et al. Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 855 . |
| MLA | Ma, Chenyang et al. "Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process" . | SCIENCE OF THE TOTAL ENVIRONMENT 855 (2023) . |
| APA | Ma, Chenyang , Zeng, Wei , Li, Jianmin , Meng, Qingan , Peng, Yongzhen . Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process . | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 855 . |
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摘要 ：
Anaerobic ammonia oxidation (Anammox) coupling sulfur autotrophic denitrification is an effective method for the advanced nitrogen removal from the wastewater with limited carbon source. The influence of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification was investigated by adding different concentrations of S2O32--S (0, 39, 78, 156 and 312 mg/L) to the Anammox system. The contribution of sulfur autotrophic denitrification and Anammox to nitrogen removal at S2O32--S concentrations of 156 mg/L was 75% similar to 83% and 17%similar to 25%, respectively, and the mixed system achieved completely nitrogen removal. However, Anammox bioactivity was completely inhibited at S2O32--S concentrations up to 312 mg/L, and only sulfur autotrophic denitrification occurred. The isotopic effects of NO2--N (delta N-15(NO2) and delta O-18(NO2)) and NO3--N (delta N-15(NO3) and delta O-18(NO3)) during Anammox coupling sulfur autotrophic denitrification showed a gradual decrease trend with the increase of S2O32--S addition. The ratios of delta N-15(NO2):delta O-18(NO2) and delta N-15(NO3):delta O-18(NO3) was maintained at 1.30-2.41 and 1.36-2.52, respectively, which revealed that Anammox was dominant nitrogen removal pathway at S2O32--S concentrations less than 156 mg/L. Microbial diversity gradually decreased with the increase of S2O32--S. The S2O32--S addition enhanced the S2O32--driven autotrophic denitrification and weakened the Anammox, leading to a gradually decreasing trend of the proportion of Candidatus Brocadia as Anammox bacteria from the initial 27% to 4% (S2O32--S of 156 mg/L). Yet Norank-f-Hydrogenophilaceae (more than 50%) and Thiobacillus (54%) as functional bacteria of autotrophic denitrification obviously increased. The appropriate amount of S2O32--S addition promoted the performance of Anammox coupling sulfur autotrophic denitrification achieved completely nitrogen removal.
关键词 ：
S2O32--driven autotrophic denitrification S2O32--driven autotrophic denitrification Isotope effect Isotope effect Anammox Anammox Complete nitrogen removal Complete nitrogen removal Microbial diversity Microbial diversity
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| GB/T 7714 | Liu, Hong , Zeng, Wei , Li, Jianmin et al. Effect of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects [J]. | WATER RESEARCH , 2022 , 218 . |
| MLA | Liu, Hong et al. "Effect of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects" . | WATER RESEARCH 218 (2022) . |
| APA | Liu, Hong , Zeng, Wei , Li, Jianmin , Zhan, Mengjia , Fan, Zhiwei , Peng, Yongzhen . Effect of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects . | WATER RESEARCH , 2022 , 218 . |
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摘要 ：
Titanium salt coagulant, as a new type of water treatment agent, has been widely studied, but most researches do not consider its effect on the biological treatment. In this study, different doses of TiCl4 were added to the biological phosphorus removal (BPR) system to investigate the impact of TiCl4 on BPR. The results showed that the addition of TiCl4 not only significantly reduced the phosphorus concentration in effluent (below 0.5 mg/L), but also kept it stable. Moreover, the sedimentation performance of activated sludge was improved, which was superior to the control group. According to the results of flow cytometry (FCM), a small amount of TiCl4 significantly improved the bioactivities, but excessive dosage caused inhibition. When the dosage of TiCl4 below 20 mg/L, polyphosphate accumulating metabolism (PAM) was strengthened. In addition, the richness of microbial community and the relative abundance of Candidatus Accumulibacter clades also increased. However, when the dosage reached 60 mg/L, the relative abundance of Candidatus Competibacter increased and the BPR system was deteriorated. This study suggests that the addition of appropriate concentration of TiCl4 can realize the synergistic enhancement of biological and chemical phosphorus removal in sewage treatment.
关键词 ：
Bacterial activity Bacterial activity Microbial community Microbial community TiCl4 TiCl4 Simultaneous chemical phosphorus removal (SCPR) Simultaneous chemical phosphorus removal (SCPR)
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| GB/T 7714 | Jia, Yuan , Zeng, Wei , Fan, Zhiwei et al. An effective titanium salt dosing strategy for phosphorus removal from wastewater: Synergistic enhancement of chemical and biological treatment [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
| MLA | Jia, Yuan et al. "An effective titanium salt dosing strategy for phosphorus removal from wastewater: Synergistic enhancement of chemical and biological treatment" . | SCIENCE OF THE TOTAL ENVIRONMENT 842 (2022) . |
| APA | Jia, Yuan , Zeng, Wei , Fan, Zhiwei , Meng, Qingan , Liu, Hongjun , Peng, Yongzhen . An effective titanium salt dosing strategy for phosphorus removal from wastewater: Synergistic enhancement of chemical and biological treatment . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
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摘要 ：
Tetrasphaera are polyphosphate accumulating organisms (PAOs) that play an important role in enhanced biological phosphorus removal (EBPR) from wastewater. The effect of a wide range of temperature changes (1-30 degrees C) on phosphorus removal, metabolism and clade-level community structure of Tetrasphaera-dominated PAOs was investigated. At 10 degrees C, the bioactivities of Tetrasphaera-dominated communities were obviously inhibited and the EBPR efficiency was only 73 %. Yet at 20-30 degrees C, EBPR efficiency reached 99 % and the relative abundance of Tetrasphaera was up to 90 %. The temperature variation changed the community distribution of Tetrasphaera clades, which was possibly a main reason for EBPR performance. Amino acids and PHA with different contents were intracellular metabolite of Tetrasphaera-dominated communities during phosphorus release and uptake at different temperatures. Moreover, Tetrasphaera fermented protein and amino acids and released VFAs. The outcomes suggested the great potential of Tetrasphaera-PAOs in the treatment of wastewater with varying temperatures and limited carbon sources.
关键词 ：
Tetrasphaera Tetrasphaera FISH FISH Temperatures Temperatures Polyphosphate accumulating organisms (PAOs) Polyphosphate accumulating organisms (PAOs) flow cytometry (FCM) flow cytometry (FCM) Enhanced biological phosphorus removal (EBPR) Enhanced biological phosphorus removal (EBPR)
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| GB/T 7714 | Liu, Hongjun , Zeng, Wei , Meng, Qingan et al. Phosphorus removal performance, intracellular metabolites and clade-level community structure of Tetrasphaera-dominated polyphosphate accumulating organisms at different temperatures [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
| MLA | Liu, Hongjun et al. "Phosphorus removal performance, intracellular metabolites and clade-level community structure of Tetrasphaera-dominated polyphosphate accumulating organisms at different temperatures" . | SCIENCE OF THE TOTAL ENVIRONMENT 842 (2022) . |
| APA | Liu, Hongjun , Zeng, Wei , Meng, Qingan , Fan, Zhiwei , Peng, Yongzhen . Phosphorus removal performance, intracellular metabolites and clade-level community structure of Tetrasphaera-dominated polyphosphate accumulating organisms at different temperatures . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
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摘要 ：
Simultaneous partial nitrification, biological phosphorous removal and sludge fermentation in continuous-flow system faced many challenges in wastewater treatment plants (WWTPs). In this study, a novel process was developed to achieve partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system dominated by Tetrasphaera treating low carbon/nitrogen ratio (COD/N) real domestic wastewater. After 80 days of operation, no external carbon was added into the influent. Nitrite accumulation ratio (NAR) reached 99.4% and removal efficiency of PO43- -P was 100%. The effluent concentration of total inorganic nitrogen (TIN) was lower than 2 mg/L. Compared with traditional biological nitrogen and phosphorus removal processes, the daily sludge discharge in continuous-flow PNPRF decreased by 61.9%. DNA concentration of the sludge supernatant after anaerobic zone was 2.26-fold of that before anaerobic zone, and percentages of intact cells decreased by 6.9% and necrotic cells increased by 8.3%, indicating that cell lysis caused the reduction of sludge discharge. The negative effect of prolonged anaerobic phase on transcriptional response of nxrB gene was more significant than that of amoA and hao gene, which resulted in stable partial nitrification. The activities of ammonium-oxidizing bacteria (AOB) were higher than that of nitrite-oxidizing bacteria (NOB), and reverse transcriptional PCR (RT-PCR) showed that RNA expression of AOB was higher than that of NOB. Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation in continuous-flow system dominated by Tetrasphaera solved the problems of carbon source deficiency and large amount of sludge discharge in treating real domestic wastewater process.
关键词 ：
Transcriptional level Transcriptional level Partial nitrification Partial nitrification Enhanced biological phosphorus removal Enhanced biological phosphorus removal Real domestic wastewater Real domestic wastewater Continuous flow Continuous flow
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| GB/T 7714 | Fan, Zhiwei , Zeng, Wei , Meng, Qingan et al. Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system and mechanism analysis at transcriptional level [J]. | CHEMICAL ENGINEERING JOURNAL , 2022 , 428 . |
| MLA | Fan, Zhiwei et al. "Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system and mechanism analysis at transcriptional level" . | CHEMICAL ENGINEERING JOURNAL 428 (2022) . |
| APA | Fan, Zhiwei , Zeng, Wei , Meng, Qingan , Liu, Hong , Ma, Chenyang , Peng, Yongzhen . Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system and mechanism analysis at transcriptional level . | CHEMICAL ENGINEERING JOURNAL , 2022 , 428 . |
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摘要 ：
本研究通过共浸渍-热解法开发了一种铈改性水葫芦生物炭吸附剂(Ce-BC),用以去除实际废水中的磷酸盐,考察了Ce-BC投加量、废水pH值、反应时间及共存的竞争性离子对吸附过程的影响.结果表明,当Ce-BC投加量为0.4 g·L-1,初始磷酸盐溶液pH值介于3～10时,Ce-BC对磷酸盐的吸附性能最佳,最大吸附量达到35.00 mg·g-1. Ce-BC对磷酸盐的吸附过程符合准二级动力学模型,并能在1 h内达到98%的磷酸盐去除率,吸附速率快.此外,Ce-BC具有较高的抗阴离子干扰能力,且具有良好的再生性能,Ce-BC经过4次再生后仍能保持90%以上的初始吸附效率.场发射扫描电镜-能量色散光谱(...
关键词 ：
吸附再生 吸附再生 固定床 固定床 氧化铈 氧化铈 水葫芦生物炭 水葫芦生物炭 磷回收 磷回收
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| GB/T 7714 | 王光泽 , 曾薇 , 李帅帅 . 铈改性水葫芦生物炭对磷酸盐的吸附特性 [J]. | 环境科学 , 2021 , 42 (10) : 4815-4825 . |
| MLA | 王光泽 et al. "铈改性水葫芦生物炭对磷酸盐的吸附特性" . | 环境科学 42 . 10 (2021) : 4815-4825 . |
| APA | 王光泽 , 曾薇 , 李帅帅 . 铈改性水葫芦生物炭对磷酸盐的吸附特性 . | 环境科学 , 2021 , 42 (10) , 4815-4825 . |
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摘要 ：
Achieving enhanced biological phosphorus removal dominated by Tetrasphaera utilizing waste activated sludge (WAS) as carbon source could solve the problems of insufficient carbon source and excessive discharge of WAS in biological phosphorus removal. Up to now, the sludge reduction ability of Tetrasphaera remained largely unknown. Furthermore, the difference between traditional sludge fermentation and sludge fermentation dominated by Tetrasphaera was still unclear. In this study, two different sequencing batch reactors (SBRs) were operated. WAS fromSBR-parent was utilized as sole carbon source to enrich Tetrasphaera with the relative abundance of 91.9% in SBR-Tetrasphaera. PO43--P removal and sludge reduction could simultaneously be achieved. The effluent concentration of PO43 --P was 0, and the sludge reduction efficiency reached about 44.14% without pretreatment of sludge. Cell integrity detected by flow cytometry, the increase of DNA concentration in the sludge supernatant and decrease of particle size of activated sludge indicated that cell death and lysis occurred in sludge reduction dominated by Tetrasphaera. Stable structure of activated sludge was also damaged in this process, which led to the sludge reduction. By analyzing the excitation-emission matrix spectra of extracellular polymeric substances and the changes of carbohydrate and protein concentration, this study proved that slowly biodegradable organics (e.g., soluble microbial byproduct, tyrosine and tryptophan aromatic protein) could be better hydrolyzed and acidized to volatile fatty acids (VFAs) in sludge fermentation dominated by Tetrasphaera than traditional sludge fermentation, which provided carbon source for biological nutrients removal and saved operation cost in wastewater treatment. (C) 2021 Elsevier B.V. All rights reserved.
关键词 ：
Biological phosphorus removal Biological phosphorus removal Enriched culture Enriched culture Fermentation Fermentation Polyphosphate accumulating organisms Polyphosphate accumulating organisms
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| GB/T 7714 | Fan, Zhiwei , Zeng, Wei , Meng, Qingan et al. Achieving enhanced biological phosphorus removal utilizing waste activated sludge as sole carbon source and simultaneous sludge reduction in sequencing batch reactor [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 799 . |
| MLA | Fan, Zhiwei et al. "Achieving enhanced biological phosphorus removal utilizing waste activated sludge as sole carbon source and simultaneous sludge reduction in sequencing batch reactor" . | SCIENCE OF THE TOTAL ENVIRONMENT 799 (2021) . |
| APA | Fan, Zhiwei , Zeng, Wei , Meng, Qingan , Liu, Hong , Liu, Hongjun , Peng, Yongzhen . Achieving enhanced biological phosphorus removal utilizing waste activated sludge as sole carbon source and simultaneous sludge reduction in sequencing batch reactor . | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 799 . |
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摘要 ：
As a novel phosphorus recovery product, vivianite (Fe-3(PO4)(2)center dot 8H(2)O) has attracted much attention due to its enormous recycling potential and foreseeable economic value. Taking sponge iron as seed material, the effect of different reaction conditions on the recovery of phosphorus in waste activated sludge by vivianite crystallization was studied. Through single factor tests, the optimal conditions for vivianite formation were in the pH range of 5.5-6.0 with Fe/P molar ratio of 1.5. Scanning electron microscopy (SEM), powder X-ray diffraction (XRD), and energy dispersive spectroscopy (EDS) were used to analyze the components of the crystals. The results showed that the vivianite produced by sponge iron as the seed crystal were larger and thicker (300-700 mu m) than other seed (200-300 mu m) and without seed (50-100 mu m). Moreover, vivianite, which was synthesized with sponge iron as seed, was obviously magnetic and could be separated from the sludge by rubidium magnet. The Box-Behnken design of the response surface methodology was used to optimize the phosphorus-recovery process with sponge iron (maximum phosphorus recovery rate was 83.17%), and the interaction effect of parameters was also examined, pH had a significant effect on the formation of vivianite. In summary, this research verifies the feasibility of using sponge iron as the seed crystal to recover phosphorus in the form of vivianite from waste activated sludge, which is conducive to the subsequent separation and utilization of vivianite.
关键词 ：
Batch tests Batch tests Anaerobic fermentation Anaerobic fermentation Box-Behnken design Box-Behnken design Crystal characterization Crystal characterization Seed crystal Seed crystal Phosphate recovery Phosphate recovery
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| GB/T 7714 | Wu, Guoding , Zeng, Wei , Li, Shuaishuai et al. Phosphorus recovery from waste activated sludge by sponge iron seeded crystallization of vivianite and process optimization with response surface methodology [J]. | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH , 2021 , 28 (41) : 58375-58386 . |
| MLA | Wu, Guoding et al. "Phosphorus recovery from waste activated sludge by sponge iron seeded crystallization of vivianite and process optimization with response surface methodology" . | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 28 . 41 (2021) : 58375-58386 . |
| APA | Wu, Guoding , Zeng, Wei , Li, Shuaishuai , Jia, Ziyue , Peng, Yongzhen . Phosphorus recovery from waste activated sludge by sponge iron seeded crystallization of vivianite and process optimization with response surface methodology . | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH , 2021 , 28 (41) , 58375-58386 . |
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摘要 ：
Preparing adsorbents with hydrated phosphorophilic metal oxide modified porous materials is a promising method for recovering phosphorus from wastewater. In this study, three kinds of adsorbents were developed by loading iron (), zirconium () and lanthanum () on diatomite under the same preparation conditions (referred to as D-HFO, D-HZO, D-HLO, respectively). The property differences and phosphorus adsorption behavior differences of three adsorbents in actual wastewater were explored. Characterization results indicated that three metals were mainly supported on diatomite in the form of hydrated oxides with nanostructures. In batch experiment, the adsorption capacity of D-HFO, D-HZO was high under acidic conditions, while D-HLO tended to adsorb phosphorus in neutral or slightly alkaline environment. The maximum adsorption capacities of D-HFO, D-HZO, D-HLO to adsorb phosphorus from wastewater were 13.22, 20.25, 148.31 mg/g, respectively. Yet in fixed-bed column experiment, D-HLO particles agglomerated due to its high chemical energy, and the maximum treatment bed-volume (BV) of D-HFO, D-HZO, D-HLO were 120, 315, 210 BV, respectively. Langmuir-Langmuir fitting results showed that the chemical precipitation contribution rate of phosphorus adsorbed on D-HFO, D-HZO, D-HLO was 83.5%, 63.8%, 86.2%, respectively. Ligand exchange and surface complexation were the main recovery mechanisms in phosphorus adsorption process from the actual wastewater by three adsorbents. © 2021 Elsevier B.V.
关键词 ：
Adsorption Adsorption Alkalinity Alkalinity Chemicals removal (water treatment) Chemicals removal (water treatment) Hydration Hydration Metal recovery Metal recovery Metals Metals Phosphorus Phosphorus Porous materials Porous materials Precipitation (chemical) Precipitation (chemical) Secondary recovery Secondary recovery
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| GB/T 7714 | Li, Shuaishuai , Zeng, Wei , Ren, Ziyan et al. Performance difference of hydrated phosphorophilic metal oxides in modifying diatomite and recovering phosphorus from wastewater [J]. | Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2021 , 623 . |
| MLA | Li, Shuaishuai et al. "Performance difference of hydrated phosphorophilic metal oxides in modifying diatomite and recovering phosphorus from wastewater" . | Colloids and Surfaces A: Physicochemical and Engineering Aspects 623 (2021) . |
| APA | Li, Shuaishuai , Zeng, Wei , Ren, Ziyan , Jia, Ziyue , Wu, Guoding , Peng, Yongzhen . Performance difference of hydrated phosphorophilic metal oxides in modifying diatomite and recovering phosphorus from wastewater . | Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2021 , 623 . |
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摘要 ：
The dual isotope fractionation of nitrogen (N) and oxygen (O) is an effective way to track the transformation of NO3--N in biological denitrification process. The Sulfur autotrophic denitrification combined with the different concentrations of S2O32- was investigated using the dual isotope fractionation of nitrogen (N) and oxygen (O) to reveal the nitrogen removal mechanism of the activated sludge. Based on successful autotrophic denitrification incubation, the modified Logistic model responded to the short-term effects of S2O32- addition on NO3--N removal and SO42- generation. Under the S2O32- addition of 0.5, 1, 2 and 4 times of the incubation stage (49.29 mg/L-394.32 mg/L), the fractionation effect of N in NO3--N ((15)epsilon(NO3)) decreased from 8.74 +/- 1.81 parts per thousand to 2.08 +/- 0.06 parts per thousand, and the fractionation effect of O in NO3--N ((18)epsilon(NO3)) declined from 11.34 +/- 0.46 parts per thousand to 5.48 +/- 0.46 parts per thousand. The (15)epsilon(NO3)/(18)epsilon(NO3) was maintained at 0.46-0.94, indicating a negative correlation between addition amount and isotope effect, and the addition of high concentrations of S2O32- was not suitable for system stabilization. Moreover, the O-18-labeled H2O (delta O-18(H2O)) tests significantly proved the presence of O exchange between NO2--N/NO3--N and H2O (67%/97%) during the nitrogen removal process, while the reoxidation of NO2--N was explored in the autotrophic denitrification. The kinetic models coupled with isotope fractionation effectively revealed the nitrogen removal characteristics in the autotrophic denitrification systems, and verified the difference between the activated sludge-based wastewater treatment process and the natural ecosystem. (C) 2021 Elsevier B.V. All rights reserved.
关键词 ：
Dual isotopes fractionation Dual isotopes fractionation Reoxidation Reoxidation Short-term effects Short-term effects Sulfur autotrophic denitrification Sulfur autotrophic denitrification The modified Logistic model The modified Logistic model
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| GB/T 7714 | Liu, Hong , Zeng, Wei , Zhan, Mengjia et al. Nitrogen removal performance of sulfur autotrophic denitrification under different S2O32- additions using isotopic fractionation of nitrogen and oxygen [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 794 . |
| MLA | Liu, Hong et al. "Nitrogen removal performance of sulfur autotrophic denitrification under different S2O32- additions using isotopic fractionation of nitrogen and oxygen" . | SCIENCE OF THE TOTAL ENVIRONMENT 794 (2021) . |
| APA | Liu, Hong , Zeng, Wei , Zhan, Mengjia , Fan, Zhiwei , Li, Jianmin , Peng, Yongzhen . Nitrogen removal performance of sulfur autotrophic denitrification under different S2O32- additions using isotopic fractionation of nitrogen and oxygen . | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 794 . |
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